Overview

  • Product name

    Anti-CoREST2 / RCOR2 antibody
  • Description

    Rabbit polyclonal to CoREST2 / RCOR2
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide conjugated to KLH derived from within residues 200 - 300 of Human CoREST2/ RCOR2.

    Read Abcam's proprietary immunogen policy (Peptide available as ab37307.)

  • Positive control

    • IHC-P: FFPE human testis tissue secions.

Properties

Applications

Our Abpromise guarantee covers the use of ab37113 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 1 µg/ml. Detects a band of approximately 45 kDa (predicted molecular weight: 58 kDa).
IHC-P Use a concentration of 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.

Target

Images

  • All lanes : Anti-CoREST2 / RCOR2 antibody (ab37113) at 1 µg/ml

    Lane 1 : MCF7 (Human breast adenocarcinoma cell line) Whole Cell Lysate
    Lane 2 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate

    Lysates/proteins at 25 µg per lane.

    Secondary
    All lanes : Goat Anti-Rabbit IgG H&L (HRP) at 1/50000 dilution

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 60 kDa
    why is the actual band size different from the predicted?


    Exposure time: 4 minutes


    This blot was produced using a 4-12% Bis-tris gel under the MOPS buffer system. The gel was run at 200V for 50 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab37113 overnight at 4°C. Antibody binding was detected using an anti-rabbit HRP secondary antibody, and visualised using ECL development solution ab133406.

     

  • IHC image of CoREST/RCOR2 staining in human testis formalin fixed paraffin embedded tissue section, performed on a Leica Bond system using the standard protocol F. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20 mins. The section was then incubated with ab37113, 1µg/ml, for 15 mins at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.

    For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.

  • Anti-CoREST2 / RCOR2 antibody (ab37113) at 1/1000 dilution + MCF7 cells

    Secondary
    HRP conjugated donkey anti-rabbit at 1/2000 dilution.

    Developed using the ECL technique.

    Performed under reducing conditions.

    Predicted band size: 58 kDa
    Observed band size: 45 kDa why is the actual band size different from the predicted?

    See Abreview

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Application
Western blot
Sample
Mouse Cell lysate - whole cell (mouse neural stem cell)
Gel Running Conditions
Reduced Denaturing (4-12%)
Loading amount
40 µg
Specification
mouse neural stem cell
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 20°C

Dr. Robert Kupp

Verified customer

Submitted Jun 27 2018

Application
Western blot
Sample
Mouse Cell lysate - whole cell (HEK293T overexpressing Flag-tagged mouse RCOR2 and)
Gel Running Conditions
Reduced Denaturing (12% SDS-PAGE)
Loading amount
60 µg
Specification
HEK293T overexpressing Flag-tagged mouse RCOR2 and
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 22°C

Abcam user community

Verified customer

Submitted Nov 23 2015

Application
Immunoprecipitation
Sample
Mouse Cell lysate - whole cell (HEK293T overexpressing Flag-tagged mouse RCOR2)
Total protein in input
50 µg
Immuno-precipitation step
Other - Prot G Dynabeads
Specification
HEK293T overexpressing Flag-tagged mouse RCOR2

Abcam user community

Verified customer

Submitted Nov 23 2015

Application
Western blot
Sample
Human Cell lysate - whole cell (MCF7)
Loading amount
100000 cells
Specification
MCF7
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 24°C

Abcam user community

Verified customer

Submitted Jul 02 2012

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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