Product nameAnti-CoREST3 / RCOR3 antibody
DescriptionRabbit polyclonal to CoREST3 / RCOR3
Tested applicationsSuitable for: WB, IP, IHC-P, ICC, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant
Synthetic peptide corresponding to a region between residue 445 and 495 of human CoREST3/ RCOR3 (NP_060724.1), (and between residues 503-553 NP_001129695.1.).
- HeLa, 293T and NIH3T3 whole cell lysates.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab76921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/2000 - 1/10000. Predicted molecular weight: 66 kDa.|
|IP||Use at 2-5 µg/mg of lysate.|
|IHC-P||1/100 - 1/500.|
|ICC||1/50 - 1/250.
Formaldehyde fixation is recommended. Permeabilization with Triton-X 100 is recommended for formaldehyde-fixed cells.
|ICC/IF||1/50 - 1/250.|
RelevanceThe CoREST3 gene encodes a functional corepressor required for regulation of neural specific gene expression. REST (RE1 silencing transcription factor 1) can mediate extraneuronal restriction by imposing either active repression via histone deacetylase recruitment or long term gene silencing using a distinct functional complex. Silencing of neuronal specific genes requires the recruitment of an associated corepressor, CoREST, that serves as a functional molecular beacon for the recruitment of molecular machinery that imposes silencing across a chromosomal interval, including transcriptional units that do not themselves contain REST response elements.
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Immunocytochemistry/Immunofluorescence analysis of NBF-fixed HeLa cells labelling CoREST3 / RCOR3 with ab76921 at 1/80 (2.5µg/ml). A DyLight® 488-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.
All lanes : Anti-CoREST3 / RCOR3 antibody (ab76921) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 66 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 1 second
Detection of CoREST3 / RCOR3 by Western Blot of Immunprecipitate.
ab76921 at 1µg/ml staining CoREST3 / RCOR3 in HeLa whole cell lysate immunoprecipitated using ab76921 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
This product has been referenced in:
- Sáez JE et al. Decreased Expression of CoREST1 and CoREST2 Together with LSD1 and HDAC1/2 during Neuronal Differentiation. PLoS One 10:e0131760 (2015). Read more (PubMed: 26111147) »
- Barrios ÁP et al. Differential properties of transcriptional complexes formed by the CoREST family. Mol Cell Biol 34:2760-70 (2014). Read more (PubMed: 24820421) »