Anti-CoREST3 / RCOR3 antibody (ab76921)
Key features and details
- Rabbit polyclonal to CoREST3 / RCOR3
- Suitable for: WB, IP, IHC-P, ICC
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CoREST3 / RCOR3 antibody -
Description
Rabbit polyclonal to CoREST3 / RCOR3 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-P, ICCmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Horse, Guinea pig, Cow, Dog, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Elephant -
Immunogen
Synthetic peptide corresponding to Human CoREST3/ RCOR3. Synthetic peptide corresponding to a region between residue 445 and 495 of human CoREST3/ RCOR3 (NP_060724.1), (and between residues 503-553 NP_001129695.1.).
Database link: Q9P2K3 -
General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading...
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Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab76921 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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WB |
1/2000 - 1/10000. Predicted molecular weight: 66 kDa.
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IP |
Use at 2-5 µg/mg of lysate.
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IHC-P |
1/200.
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ICC |
1/50 - 1/250.
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Notes |
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WB
1/2000 - 1/10000. Predicted molecular weight: 66 kDa. |
IP
Use at 2-5 µg/mg of lysate. |
IHC-P
1/200. |
ICC
1/50 - 1/250. |
Target
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Relevance
The CoREST3 gene encodes a functional corepressor required for regulation of neural specific gene expression. REST (RE1 silencing transcription factor 1) can mediate extraneuronal restriction by imposing either active repression via histone deacetylase recruitment or long term gene silencing using a distinct functional complex. Silencing of neuronal specific genes requires the recruitment of an associated corepressor, CoREST, that serves as a functional molecular beacon for the recruitment of molecular machinery that imposes silencing across a chromosomal interval, including transcriptional units that do not themselves contain REST response elements. -
Cellular localization
Nuclear -
Database links
- Entrez Gene: 55758 Human
- Entrez Gene: 214742 Mouse
- SwissProt: Q9P2K3 Human
- SwissProt: Q6PGA0 Mouse
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Alternative names
- 4921514E24Rik antibody
- C730034D20Rik antibody
- E130101E15Rik antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human testicular seminoma tissue labelling CoREST3 / RCOR3 with ab76921 at 1/200 dilution.
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Immunocytochemistry/Immunofluorescence analysis of NBF-fixed HeLa cells labelling CoREST3 / RCOR3 with ab76921 at 1/80 (2.5µg/ml). A DyLight® 488-conjugated goat anti-rabbit IgG (H+L) (1/100) was used as the secondary antibody.
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All lanes : Anti-CoREST3 / RCOR3 antibody (ab76921) at 0.04 µg/ml
Lane 1 : HeLa whole cell lysate at 50 µg
Lane 2 : HeLa whole cell lysate at 15 µg
Lane 3 : HeLa whole cell lysate at 5 µg
Lane 4 : 293T whole cell lysate at 50 µg
Lane 5 : NIH3T3 whole cell lysate at 50 µg
Predicted band size: 66 kDa
Observed band size: 70 kDa why is the actual band size different from the predicted?
Exposure time: 1 second -
Detection of CoREST3 / RCOR3 by Western Blot of Immunprecipitate.
ab76921 at 1µg/ml staining CoREST3 / RCOR3 in HeLa whole cell lysate immunoprecipitated using ab76921 at 3µg/mg lysate (1 mg/IP; 20% of IP loaded/lane).
Detection: Chemiluminescence with exposure time of 10 seconds.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (3)
ab76921 has been referenced in 3 publications.
- Sáez JE et al. PIAS? controls stability and facilitates SUMO-2 conjugation to CoREST family of transcriptional co-repressors. Biochem J 475:1441-1454 (2018). PubMed: 29555846
- Sáez JE et al. Decreased Expression of CoREST1 and CoREST2 Together with LSD1 and HDAC1/2 during Neuronal Differentiation. PLoS One 10:e0131760 (2015). PubMed: 26111147
- Barrios ÁP et al. Differential properties of transcriptional complexes formed by the CoREST family. Mol Cell Biol 34:2760-70 (2014). PubMed: 24820421