Key features and details
- Rabbit polyclonal to CORO1B
- Suitable for: WB, ICC/IF
- Reacts with: Mouse, Rabbit, Human
- Isotype: IgG
Product nameAnti-CORO1B antibody
See all CORO1B primary antibodies
DescriptionRabbit polyclonal to CORO1B
Tested applicationsSuitable for: WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Rabbit, Human
Predicted to work with: Rat
Synthetic peptide, coupled to carrier protein, corresponding to amino acids at the C terminus of Human CORO1B.
- Human A431 and HeLa cell lysates; Mouse brain extracts and Rabbit spleen fibroblasts.
General notesDo not aliquot.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
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In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at -20°C. Stable for 12 months at -20°C.
Storage bufferPreservative: 0.05% Sodium azide
Constituents: 49% PBS, 50% Glycerol, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab119714 was affinity purified using CORO1B (C-terminus) peptide (without carrier).
Our Abpromise guarantee covers the use of ab119714 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/1000. Predicted molecular weight: 54 kDa.|
FunctionRegulates leading edge dynamics and cell motility in fibroblasts. May be involved in cytokinesis and signal transduction.
Sequence similaritiesBelongs to the WD repeat coronin family.
Contains 5 WD repeats.
modificationsPhosphorylation by PKC on Ser-2 regulates the interaction with the Arp2/3 complex and cell motility in fibroblasts. Phosphorylation does not seem to affect subcellular location.
Cellular localizationCytoplasm > cytoskeleton. Localized to the leading edge in fibroblasts, as well as weakly along actin stress fibers.
- Information by UniProt
- COR1B_HUMAN antibody
- CORO 1B antibody
- CORO1B antibody
Lanes 1-2 : Anti-CORO1B antibody (ab119714) at 1/1000 dilution
Lanes 3-4 : Anti-CORO1B (phospho S2) antibody (ab119705) at 1/1000 dilution
Lanes 1 & 3 : Human A431 cells treated with
Calyculin A (100 nM) for 30 min
Lanes 2 & 4 : Human A431 cells treated with
Calyculin A (100 nM) for 30 min before
treatment with lambda phosphatase
Predicted band size: 54 kDa
ab119714 at 1/100 dilution staining CORO1B in Rabbit spleen fibroblasts treated with Calyculin A in the absence (left) or presence (right) of blocking peptide. Detection used an appropriate secondary antibody conjugated to Cy3.
ab119714 has not yet been referenced specifically in any publications.