Overview

  • Product name

  • Description

    Rabbit polyclonal to CORO2B
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Human
    Predicted to work with: Xenopus laevis, Xenopus tropicalis
  • Immunogen

    Recombinant fragment, corresponding to a region within amino acids 1-237 of Human CORO2B.

  • Positive control

    • NT2D1, IMR32, U87-MG, MCF-7 and NIH-3T3 cell lysates; H441 xenograft; MCF-7 cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab151327 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 55 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
ICC/IF 1/100 - 1/1000.

Target

Images

  • Anti-CORO2B antibody (ab151327) at 1/1000 dilution + U87-MG whole cell lysate at 30 µg

    Predicted band size: 55 kDa



    7.5% SDS PAGE
  • Anti-CORO2B antibody (ab151327) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg

    Predicted band size: 55 kDa



    10% SDS PAGE
  • Immunohistochemical analysis of paraffin-embedded H441 xenograft labeling CORO2B with ab151327 at 1/100 dilution.
  • Immunofluorescent analysis of methanol-fixed MCF-7 cells labeling CORO2B with ab151327 at 1/200 dilution. Right panel co-stained with Hoechst 33342.

References

ab151327 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

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