Anti-CORO2B antibody (ab151327)
Key features and details
- Rabbit polyclonal to CORO2B
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Overview
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Product name
Anti-CORO2B antibody -
Description
Rabbit polyclonal to CORO2B -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Human
Predicted to work with: Xenopus laevis, Xenopus tropicalis
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Immunogen
Recombinant fragment, corresponding to a region within amino acids 1-237 of Human CORO2B.
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Positive control
- NT2D1, IMR32, U87-MG, MCF-7 and NIH-3T3 cell lysates; H441 xenograft; MCF-7 cells.
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General notes
The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab151327 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB |
1/500 - 1/3000. Predicted molecular weight: 55 kDa.
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| IHC-P |
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0).
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| ICC/IF |
1/100 - 1/1000.
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| Notes |
|---|
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WB
1/500 - 1/3000. Predicted molecular weight: 55 kDa. |
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IHC-P
1/100 - 1/1000. Perform heat mediated antigen retrieval before commencing with IHC staining protocol using 10mM Citrate buffer (pH6.0) or Tris-EDTA buffer (pH8.0). |
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ICC/IF
1/100 - 1/1000. |
Target
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Function
May play a role in the reorganization of neuronal actin structure. -
Tissue specificity
Expressed predominantly in brain. -
Sequence similarities
Belongs to the WD repeat coronin family.
Contains 5 WD repeats. -
Cellular localization
Cytoplasm > cytoskeleton. - Information by UniProt
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Database links
- Entrez Gene: 10391 Human
- Entrez Gene: 235431 Mouse
- Entrez Gene: 444835 Xenopus laevis
- Entrez Gene: 549719 Xenopus tropicalis
- Omim: 605002 Human
- SwissProt: Q9UQ03 Human
- SwissProt: Q8BH44 Mouse
- SwissProt: Q6DJD8 Xenopus laevis
see all -
Alternative names
- Actin binding protein antibody
- Clipin-C antibody
- CLIPINC antibody
see all
Images
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Western blot - Anti-CORO2B antibody (ab151327)Anti-CORO2B antibody (ab151327) at 1/1000 dilution + U87-MG whole cell lysate at 30 µg
Predicted band size: 55 kDa
7.5% SDS PAGE -
Western blot - Anti-CORO2B antibody (ab151327)Anti-CORO2B antibody (ab151327) at 1/1000 dilution + NIH-3T3 whole cell lysate at 30 µg
Predicted band size: 55 kDa
10% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-CORO2B antibody (ab151327)Immunohistochemical analysis of paraffin-embedded H441 xenograft labeling CORO2B with ab151327 at 1/100 dilution. -
Immunocytochemistry/ Immunofluorescence - Anti-CORO2B antibody (ab151327)Immunofluorescent analysis of methanol-fixed MCF-7 cells labeling CORO2B with ab151327 at 1/200 dilution. Right panel co-stained with Hoechst 33342.
Protocols
Datasheets and documents
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SDS download
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Datasheet download
References (0)
ab151327 has not yet been referenced specifically in any publications.