Anti-Coronin 1a/TACO antibody (ab72212)
Key features and details
- Rabbit polyclonal to Coronin 1a/TACO
- Suitable for: WB, IP, IHC-P
- Reacts with: Human
- Isotype: IgG
Overview
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Product name
Anti-Coronin 1a/TACO antibody
See all Coronin 1a/TACO primary antibodies -
Description
Rabbit polyclonal to Coronin 1a/TACO -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
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Immunogen
Synthetic peptide corresponding to Human Coronin 1a/TACO.
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Positive control
- Jurkat whole cell lysate (ab7899)
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General notes
This product was previously labelled as Coronin 1a
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 6.8
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab72212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. -
Tissue specificity
Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut. -
Sequence similarities
Belongs to the WD repeat coronin family.
Contains 7 WD repeats. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle > phagosome membrane. In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification. - Information by UniProt
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Database links
- Entrez Gene: 11151 Human
- Omim: 605000 Human
- SwissProt: P31146 Human
- Unigene: 415067 Human
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Alternative names
- Actin binding protein antibody
- CLABP antibody
- Clipin A antibody
see all
Images
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Western blot - Anti-Coronin 1a/TACO antibody (ab72212)All lanes : Anti-Coronin 1a/TACO antibody (ab72212) at 0.04 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody (ab72212)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling Coronin 1a/TACO with ab72212 at 1/1000 (0.2µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-Coronin 1a/TACO antibody (ab72212)Immunoprecipitation analysis of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded). ab72212 used for IP at 3 µg/mg lysate and for WB at 1 µg/ml. Lane 1 - ab72212 IP. Lane 2 - control IgG.
Protocols
Datasheets and documents
References (1)
ab72212 has been referenced in 1 publication.
- Riley DRJ et al. Coronin 1 Is Required for Integrin ß2 Translocation in Platelets. Int J Mol Sci 21:N/A (2020). PubMed: 31948107
