Anti-Coronin 1a/TACO antibody (ab72212)
- Datasheet
- References
- Protocols
Overview
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Product name
Anti-Coronin 1a/TACO antibody
See all Coronin 1a/TACO primary antibodies -
Description
Rabbit polyclonal to Coronin 1a/TACO -
Host species
Rabbit -
Tested applications
Suitable for: WB, IP, IHC-Pmore details -
Species reactivity
Reacts with: Human
Predicted to work with: Horse, Cow, Dog, Pig, Chimpanzee, Rhesus monkey, Gorilla, Orangutan, Bat, Elephant
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Immunogen
Synthetic peptide corresponding to Human Coronin 1a/TACO.
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Positive control
- Jurkat whole cell lysate (ab7899)
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General notes
This product was previously labelled as Coronin 1a
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: 0.1% BSA, Tris buffered saline -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab72212 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/2000 - 1/10000. Detects a band of approximately 55 kDa (predicted molecular weight: 51 kDa). | |
| IP | Use at 2-5 µg/mg of lysate. | |
| IHC-P | 1/200 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes. -
Tissue specificity
Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut. -
Sequence similarities
Belongs to the WD repeat coronin family.
Contains 7 WD repeats. -
Cellular localization
Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle > phagosome membrane. In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification. - Information by UniProt
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Database links
- Entrez Gene: 11151 Human
- Omim: 605000 Human
- SwissProt: P31146 Human
- Unigene: 415067 Human
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Alternative names
- Actin binding protein antibody
- CLABP antibody
- Clipin A antibody
see all
Images
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Western blot - Anti-Coronin 1a/TACO antibody (ab72212)All lanes : Anti-Coronin 1a/TACO antibody (ab72212) at 0.04 µg/ml
Lane 1 : Jurkat whole cell lysate at 50 µg
Lane 2 : Jurkat whole cell lysate at 15 µg
Lane 3 : Jurkat whole cell lysate at 5 µg
Developed using the ECL technique.
Predicted band size: 51 kDa
Exposure time: 3 minutes -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody (ab72212)Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human stomach carcinoma tissue labelling Coronin 1a/TACO with ab72212 at 1/1000 (0.2µg/ml). Detection: DAB.
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Immunoprecipitation - Anti-Coronin 1a/TACO antibody (ab72212)Immunoprecipitation analysis of Jurkat whole cell lysate (1 mg for IP, 20% of IP loaded). ab72212 used for IP at 3 µg/mg lysate and for WB at 1 µg/ml. Lane 1 - ab72212 IP. Lane 2 - control IgG.
Datasheets and documents
References
ab72212 has not yet been referenced specifically in any publications.
