Overview

  • Product name

    Anti-Coronin 1a/TACO antibody - C-terminal
    See all Coronin 1a/TACO primary antibodies
  • Description

    Rabbit polyclonal to Coronin 1a/TACO - C-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Cow
  • Immunogen

    Synthetic peptide within Human Coronin 1a/TACO (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
    Database link: P31146

  • Positive control

    • WB: U87-MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. ICC/IF: HepG2 cells. IHC-P: Human gastric cancer; mouse and rat brain tissue. Mouse duodenum and rat intestine tissue. IHC-Fr: Mouse cerebellum tissue.
  • General notes

     This product was previously labelled as Coronin 1a

     

Properties

Applications

Our Abpromise guarantee covers the use of ab228635 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 51 kDa.
IHC-P 1/100 - 1/1000.
IHC-Fr 1/100 - 1/1000.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes.
  • Tissue specificity

    Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut.
  • Sequence similarities

    Belongs to the WD repeat coronin family.
    Contains 7 WD repeats.
  • Cellular localization

    Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle > phagosome membrane. In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification.
  • Information by UniProt
  • Database links

  • Alternative names

    • Actin binding protein antibody
    • CLABP antibody
    • Clipin A antibody
    • Clipin-A antibody
    • CLIPINA antibody
    • COR1A_HUMAN antibody
    • CORO1A antibody
    • Coronin 1 antibody
    • Coronin actin binding protein 1A antibody
    • Coronin like protein A antibody
    • Coronin like protein p57 antibody
    • Coronin-1A antibody
    • Coronin-like protein A antibody
    • Coronin-like protein p57 antibody
    • FLJ41407 antibody
    • HCORO1 antibody
    • MGC117380 antibody
    • OTTHUMP00000163017 antibody
    • p57 antibody
    • TACO antibody
    • Tryptophan aspartate containing coat protein antibody
    • Tryptophan aspartate-containing coat protein antibody
    see all

Images

  • All lanes : Anti-Coronin 1a/TACO antibody - C-terminal (ab228635) at 1/1000 dilution

    Lane 1 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell extract
    Lane 2 : SK-N-SH (Human neuroblastoma cell line) whole cell extract
    Lane 3 : IMR32 (human neuroblast cell line) whole cell extract
    Lane 4 : SK-N-AS (Human brain neuroblastoma cell line) whole cell extracts

    Lysates/proteins at 30 µg per lane.

    Developed using the ECL technique.

    Predicted band size: 51 kDa



    10% SDS-PAGE gel.

  • Frozen-sectioned adult mouse cerebellum stained for Coronin 1A/TACO Protein (green) using ab228635 at 1/500 in immunohistochemical analysis.

    NF-H (Neurofilament Heavy) cells stained for NF-H (red) using NF-H antibody at 1/500 dilution.

    Counterstain: Fluoroshield with DAPI (blue).

     

  • Paraffin-embedded rat intestine tissue stained for Coronin 1A/TACO protein using ab228635 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse duodenum tissue stained for Coronin 1A/TACO using ab228635 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded rat brain tissue stained for Coronin 1A/TACO using ab228635 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded mouse brain tissue stained for Coronin 1A/TACO using ab228635 at 1/500 dilution in immunohistochemical analysis.

  • Paraffin-embedded human gastric cancer tissue stained for Coronin 1A/TACO using ab228635 at 1/100 dilution in immunohistochemical analysis.

  • Methanol-fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for Coronin 1A/TACO (green) using ab228635 at 1/500 dilution in ICC/IF.

    Nuclear counterstain: Hoechst 33342 (blue).

References

ab228635 has not yet been referenced specifically in any publications.

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