Key features and details
- Rabbit polyclonal to Coronin 1a/TACO - C-terminal
- Suitable for: WB, IHC-P, IHC-Fr, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Product nameAnti-Coronin 1a/TACO antibody - C-terminal
See all Coronin 1a/TACO primary antibodies
DescriptionRabbit polyclonal to Coronin 1a/TACO - C-terminal
Tested applicationsSuitable for: WB, IHC-P, IHC-Fr, ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Predicted to work with: Cow
Synthetic peptide within Human Coronin 1a/TACO (C terminal). The exact sequence is proprietary. Conjugated to a carrier protein.
Database link: P31146
- WB: U87-MG, SK-N-SH, IMR32 and SK-N-AS whole cell extracts. ICC/IF: HepG2 cells. IHC-P: Human gastric cancer; mouse and rat brain tissue. Mouse duodenum and rat intestine tissue. IHC-Fr: Mouse cerebellum tissue.
This product was previously labelled as Coronin 1a
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.00
Preservative: 0.01% Thimerosal (merthiolate)
Constituents: 1.21% Tris, 0.75% Glycine, 10% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab228635 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|WB||1/500 - 1/3000. Predicted molecular weight: 51 kDa.|
|IHC-P||1/100 - 1/1000.|
|IHC-Fr||1/100 - 1/1000.|
|ICC/IF||1/100 - 1/1000.|
FunctionMay be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes.
Tissue specificityExpressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut.
Sequence similaritiesBelongs to the WD repeat coronin family.
Contains 7 WD repeats.
Cellular localizationCytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle > phagosome membrane. In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification.
- Information by UniProt
- Actin binding protein antibody
- CLABP antibody
- Clipin A antibody
All lanes : Anti-Coronin 1a/TACO antibody - C-terminal (ab228635) at 1/1000 dilution
Lane 1 : U87-MG (Human glioblastoma-astrocytoma epithelial cell line) whole cell extract
Lane 2 : SK-N-SH (Human neuroblastoma cell line) whole cell extract
Lane 3 : IMR32 (human neuroblast cell line) whole cell extract
Lane 4 : SK-N-AS (Human brain neuroblastoma cell line) whole cell extracts
Lysates/proteins at 30 µg per lane.
Developed using the ECL technique.
Predicted band size: 51 kDa
10% SDS-PAGE gel.
Frozen-sectioned adult mouse cerebellum stained for Coronin 1A/TACO Protein (green) using ab228635 at 1/500 in immunohistochemical analysis.
NF-H (Neurofilament Heavy) cells stained for NF-H (red) using NF-H antibody at 1/500 dilution.
Counterstain: Fluoroshield with DAPI (blue).
Paraffin-embedded rat intestine tissue stained for Coronin 1A/TACO protein using ab228635 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded mouse duodenum tissue stained for Coronin 1A/TACO using ab228635 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded rat brain tissue stained for Coronin 1A/TACO using ab228635 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded mouse brain tissue stained for Coronin 1A/TACO using ab228635 at 1/500 dilution in immunohistochemical analysis.
Paraffin-embedded human gastric cancer tissue stained for Coronin 1A/TACO using ab228635 at 1/100 dilution in immunohistochemical analysis.
Methanol-fixed HepG2 (Human liver hepatocellular carcinoma cell line) cells stained for Coronin 1A/TACO (green) using ab228635 at 1/500 dilution in ICC/IF.
Nuclear counterstain: Hoechst 33342 (blue).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab228635 has not yet been referenced specifically in any publications.