RecombinantRabMAb

Recombinant Anti-Coronin 1a/TACO antibody [EPR19467-81] - BSA and Azide free (ab243566)

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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-81] - BSA and Azide free (ab243566)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-81] - BSA and Azide free (ab243566)
  • Flow Cytometry - Anti-Coronin 1a/TACO antibody [EPR19467-81] - BSA and Azide free (ab243566)
  • Immunocytochemistry/ Immunofluorescence - Anti-Coronin 1a/TACO antibody [EPR19467-81] - BSA and Azide free (ab243566)
  • Anti-Coronin 1a/TACO antibody [EPR19467-81] - BSA and Azide free (ab243566)

Key features and details

  • Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
  • Rabbit monoclonal [EPR19467-81] to Coronin 1a/TACO - BSA and Azide free
  • Suitable for: IHC-P, WB, ICC/IF, Flow Cyt
  • Reacts with: Human

Overview

  • Product name

    Anti-Coronin 1a/TACO antibody [EPR19467-81] - BSA and Azide free
    See all Coronin 1a/TACO primary antibodies

  • Description

    Rabbit monoclonal [EPR19467-81] to Coronin 1a/TACO - BSA and Azide free

  • Host species

    Rabbit

  • Tested applications

    Suitable for: IHC-P, WB, ICC/IF, Flow Cytmore details

  • Species reactivity

    Reacts with: Human

  • Immunogen

    Recombinant fragment within Human Coronin 1a/TACO aa 350 to the C-terminus. The exact sequence is proprietary.
    Database link: P31146

  • Positive control

    • IHC-P: Human colon and spleen tissue. ICC/IF: Jurkat cells. Flow cyt: Jurkat cells.

  • General notes

    ab243566 is the carrier-free version of ab243090 This format is designed for use in antibody labeling, including fluorochromes, metal isotopes, oligonucleotides, enzymes.

     

    Our carrier-free formats are supplied in a buffer free of BSA, sodium azide and glycerol for higher conjugation efficiency.

    Use our conjugation kits  for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.

    Ab243566 is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm.

    Maxpar® is a trademark of Fluidigm Canada Inc.

    This product was previously labelled as Coronin 1a

    This product is a recombinant monoclonal antibody, which offers several advantages including:

    • - High batch-to-batch consistency and reproducibility
    • - Improved sensitivity and specificity
    • - Long-term security of supply
    • - Animal-free production
    For more information see here.

    Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

Applications

Our Abpromise guarantee covers the use of ab243566 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IHC-P Use at an assay dependent concentration. Perform heat mediated antigen retrieval with Tris/EDTA buffer pH 9.0 before commencing with IHC staining protocol.
WB Use at an assay dependent concentration. Detects a band of approximately 51, 57 kDa (predicted molecular weight: 51 kDa).
ICC/IF Use at an assay dependent concentration.
Flow Cyt Use at an assay dependent concentration.

Target

  • Function

    May be a crucial component of the cytoskeleton of highly motile cells, functioning both in the invagination of large pieces of plasma membrane, as well as in forming protrusions of the plasma membrane involved in cell locomotion. In mycobacteria-infected cells, its retention on the phagosomal membrane prevents fusion between phagosomes and lysosomes.

  • Tissue specificity

    Expressed in brain, thymus, spleen, bone marrow and lymph node. Low in lung and gut.

  • Sequence similarities

    Belongs to the WD repeat coronin family.
    Contains 7 WD repeats.

  • Cellular localization

    Cytoplasm > cytoskeleton. Cytoplasm > cell cortex. Cytoplasmic vesicle > phagosome membrane. In non-infected macrophages, associated with the cortical microtubule network. In mycobacteria-infected macrophages, becomes progressively relocalized and retained around the mycobacterial phagosomes. Retention on the phagosomal membrane is strictly dependent on mycobacterial viability and not due to impaired acidification.
  • Information by UniProt

  • Database links

    • Alternative names

      • Actin binding protein antibody
      • CLABP antibody
      • Clipin A antibody
      • Clipin-A antibody
      • CLIPINA antibody
      • COR1A_HUMAN antibody
      • CORO1A antibody
      • Coronin 1 antibody
      • Coronin actin binding protein 1A antibody
      • Coronin like protein A antibody
      • Coronin like protein p57 antibody
      • Coronin-1A antibody
      • Coronin-like protein A antibody
      • Coronin-like protein p57 antibody
      • FLJ41407 antibody
      • HCORO1 antibody
      • MGC117380 antibody
      • OTTHUMP00000163017 antibody
      • p57 antibody
      • TACO antibody
      • Tryptophan aspartate containing coat protein antibody
      • Tryptophan aspartate-containing coat protein antibody
      see all

    Images

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-81] - BSA and Azide free (ab243566)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-81] - BSA and Azide free (ab243566)

      Immunohistochemical analysis of paraffin-embedded human spleen tissue labeling Coronin 1a/TACO with ab243090 at 1/1000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human spleen (PMID: 17341475) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody isGoat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243090).

    • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-81] - BSA and Azide free (ab243566)
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-Coronin 1a/TACO antibody [EPR19467-81] - BSA and Azide free (ab243566)

      Immunohistochemical analysis of paraffin-embedded human colon tissue labeling Coronin 1a/TACO with ab243090 at 1/2000 dilution, followed by a Goat Anti-Rabbit IgG H&L (HRP) ready to use. Positive staining on human colon (PMID: 17341475) is observed. Counter stained with hematoxylin.

      Secondary antibody only control: Used PBS instead of primary antibody, secondary antibody isGoat Anti-Rabbit IgG H&L (HRP) ready to use.

      Heat mediated antigen retrieval using ab93684 (Tris/EDTA buffer, pH 9.0).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243090).

    • Flow Cytometry - Anti-Coronin 1a/TACO antibody [EPR19467-81] - BSA and Azide free (ab243566)
      Flow Cytometry - Anti-Coronin 1a/TACO antibody [EPR19467-81] - BSA and Azide free (ab243566)

      Flow cytometric analysis of 4% paraformaldehyde-fixed, 90% methanol-permeabilized HEK-293 (human epithelial cell line from embryonic kidney) (Left panel) and Jurkat (human epithelial cell line from embryonic kidney) (Right panel) cells labeling Coronin 1a/TACO with ab243090 at 1/600 dilution (red) compared with a Rabbit IgG, monoclonal [EPR25A] - Isotype Control (ab172730) (black) and an unlabelled control (cells without incubation with primary antibody and secondary antibody) (blue). Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077), at 1/2000 dilution was used as the secondary antibody.

       

      Negative control: HEK-293 (PMID:17341475).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243090).

    • Immunocytochemistry/ Immunofluorescence - Anti-Coronin 1a/TACO antibody [EPR19467-81] - BSA and Azide free (ab243566)
      Immunocytochemistry/ Immunofluorescence - Anti-Coronin 1a/TACO antibody [EPR19467-81] - BSA and Azide free (ab243566)

      Immunofluorescent analysis of 4% paraformaldehyde-fixed, 0.1% Triton X-100 permeabilized Jurkat (human T cell leukemia cell line from peripheral blood) cells labeling Coronin 1a/TACO with ab243090 at 1/100 dilution, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution (green). Confocal image showing cytoplasmic and membranous staining in Jurkat cell line is observed. The nuclear counter stain is DAPI (blue). Tubulin is detected with Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor® 594) (ab195889) at 1/200 dilution.

      Control: Used PBS instead of primary antibody, followed by Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077) secondary antibody at 1/1000 dilution.

      Negative control: HEK-293 (PMID:17341475).

      This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab243090).

    • Anti-Coronin 1a/TACO antibody [EPR19467-81] - BSA and Azide free (ab243566)
      Anti-Coronin 1a/TACO antibody [EPR19467-81] - BSA and Azide free (ab243566)

    Protocols

    To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

    Click here to view the general protocols

    References (0)

    Publishing research using ab243566? Please let us know so that we can cite the reference in this datasheet.

    ab243566 has not yet been referenced specifically in any publications.

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