Key features and details
- Goat polyclonal to Coronin 3
- Suitable for: ICC/IF, WB
- Reacts with: Mouse, Human
- Isotype: IgG
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: Tris buffered saline, 0.5% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesPurified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Our Abpromise guarantee covers the use of ab15719 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use at an assay dependent concentration. PubMed: 20362548|
|WB||Use a concentration of 0.2 - 1 µg/ml. Detects a band of approximately 55 kDa (predicted molecular weight: 55 kDa).
A 1 hour primary incubation is recommended for this product.
FunctionMay be involved in cytokinesis, motility, and signal transduction.
Sequence similaritiesBelongs to the WD repeat coronin family.
Contains 4 WD repeats.
- Information by UniProt
- COR1C_HUMAN antibody
- CORO 1C antibody
- Coro1c antibody
Ab15719 at 0.2
µg/ml staining Coronin 3 in human lung lysate by Western blot (ECL)
Anti-Coronin 3 antibody (ab15719) at 1/1000 dilution + Mouse 3T3 fibroblast whole cell lysate (25ug)
HRP conjugated bovine anti-goat IgG
Predicted band size: 55 kDa
Observed band size: 55 kDa
This blot shows significant non-specific background staining. Increasing the length of the blocking step and including a blocking agent in the primary antibody solution may reduce this.
ab15719 has been referenced in 2 publications.
- Kimura T et al. Glucose-induced translocation of coronin 3 regulates the retrograde transport of the secretory membrane in the pancreatic beta-cells. Biochem Biophys Res Commun 395:318-23 (2010). ICC/IF ; Mouse . PubMed: 20362548
- Kimura T et al. The GDP-dependent Rab27a effector coronin 3 controls endocytosis of secretory membrane in insulin-secreting cell lines. J Cell Sci 121:3092-8 (2008). PubMed: 18768935