Product nameAnti-Coronin-7 antibody
See all Coronin-7 primary antibodies
DescriptionRabbit polyclonal to Coronin-7
Tested applicationsSuitable for: WB, IPmore details
Species reactivityReacts with: Human
Synthetic peptide within Human Coronin-7 aa 400-450. The exact sequence is proprietary.
Database link: P57737
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: Coronin-7 IP in HeLa whole cell lysate.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferPreservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA
Concentration information loading...
PurityImmunogen affinity purified
Purification notesab241291 was affinity purified using an epitope specific to Coronin-7 immobilized on solid support
Our Abpromise guarantee covers the use of ab241291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IP||Use at an assay dependent concentration.
20 µl/mg lysate
FunctionMay play a role in the maintenance of the Golgi apparatus morphology and in the protein export from the Golgi.
Sequence similaritiesBelongs to the WD repeat coronin family.
Contains 8 WD repeats.
modificationsThe membrane-associated form is phosphorylated on tyrosine residues.
Cellular localizationCytoplasm > cytosol. Golgi apparatus membrane. Cytoplasmic vesicle. Predominantly cytosolic. Detected on vesicle-like cytoplasmic structures and on the cis-Golgi. Not associated with actin filaments.
- Information by UniProt
- 0610011B16Rik antibody
- 70 kDa WD repeat tumor rejection antigen homolog antibody
- CORO 7 antibody
All lanes : Anti-Coronin-7 antibody (ab241291) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 seconds
Prepared using NETN lysis buffer.
Coronin-7 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241291 used for IP at 20 µl per reaction. For WB at a 1:100 dilution.
Lane 1: ab241291 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Chemiluminescence detection: 30 seconds.
ab241291 has not yet been referenced specifically in any publications.