Anti-Coronin-7 antibody (ab241291)
- Datasheet
- References
- Protocols
Overview
-
Product name
Anti-Coronin-7 antibody
See all Coronin-7 primary antibodies -
Description
Rabbit polyclonal to Coronin-7 -
Host species
Rabbit -
Tested applications
Suitable for: WB, IPmore details -
Species reactivity
Reacts with: Human -
Immunogen
Synthetic peptide within Human Coronin-7 aa 400-450. The exact sequence is proprietary.
Database link: P57737 -
Positive control
- WB: HeLa, HEK-293T and Jurkat whole cell lysate. IP: Coronin-7 IP in HeLa whole cell lysate.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle. -
Storage buffer
Preservative: 0.09% Sodium azide
Constituents: Tris buffered saline, 0.1% BSA -
Concentration information loading... -
Purity
Immunogen affinity purified -
Purification notes
ab241291 was affinity purified using an epitope specific to Coronin-7 immobilized on solid support -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
-
Compatible Secondaries
-
Isotype control
-
Positive Controls
Applications
Our Abpromise guarantee covers the use of ab241291 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/1000. | |
| IP | Use at an assay dependent concentration. 20 µl/mg lysate |
Target
-
Function
May play a role in the maintenance of the Golgi apparatus morphology and in the protein export from the Golgi. -
Sequence similarities
Belongs to the WD repeat coronin family.
Contains 8 WD repeats. -
Post-translational
modificationsThe membrane-associated form is phosphorylated on tyrosine residues. -
Cellular localization
Cytoplasm > cytosol. Golgi apparatus membrane. Cytoplasmic vesicle. Predominantly cytosolic. Detected on vesicle-like cytoplasmic structures and on the cis-Golgi. Not associated with actin filaments. - Information by UniProt
-
Database links
- Entrez Gene: 79585 Human
- Omim: 611668 Human
- SwissProt: P57737 Human
- Unigene: 437957 Human
-
Alternative names
- 0610011B16Rik antibody
- 70 kDa WD repeat tumor rejection antigen homolog antibody
- CORO 7 antibody
see all
Images
-
Western blot - Anti-Coronin-7 antibody (ab241291)All lanes : Anti-Coronin-7 antibody (ab241291) at 1/1000 dilution
Lane 1 : HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate
Lane 2 : HEK-293T (Human epithelial cell line from embryonic kidney transformed with large T antigen) whole cell lysate
Lane 3 : Jurkat (Human T cell leukemia cell line from peripheral blood) whole cell lysate
Lysates/proteins at 50 µg per lane.
Exposure time: 30 secondsPrepared using NETN lysis buffer.
-
Immunoprecipitation - Anti-Coronin-7 antibody (ab241291)Coronin-7 was immunoprecipitated from HeLa (Human epithelial cell line from cervix adenocarcinoma) whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) prepared using NETN lysis buffer.
ab241291 used for IP at 20 µl per reaction. For WB at a 1:100 dilution.
Lane 1: ab241291 IP in HeLa whole cell lysate.
Lane 2: Control IgG.
Chemiluminescence detection: 30 seconds.
Datasheets and documents
References
ab241291 has not yet been referenced specifically in any publications.
