Key features and details
- Rabbit polyclonal to Cortactin
- Suitable for: IHC-P, WB
- Reacts with: Human
- Isotype: IgG
Product nameAnti-Cortactin antibody
See all Cortactin primary antibodies
DescriptionRabbit polyclonal to Cortactin
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide within Human Cortactin aa 388-437. The exact sequence is proprietary.
Database link: Q14247
- IHC-P: Human kidney tissue. WB: HeLa cells.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 0.87% Sodium chloride, 50% Glycerol
(PBS without Mg2+ and Ca2+).
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab230910 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/33 - 1/50.|
|WB||1/500 - 1/1000. Predicted molecular weight: 62 kDa.|
FunctionContributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
Sequence similaritiesContains 7 cortactin repeats.
Contains 1 SH3 domain.
DomainThe SH3 motif may mediate binding to the cytoskeleton.
modificationsTyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
Cellular localizationCytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia.
- Information by UniProt
- Amplaxin antibody
- CTTN antibody
- EMS 1 antibody
Formalin-fixed, paraffin-embedded human kidney tissue stained for Cortactin using ab230910 at 1/33 dilution in immunohistochemical analysis.
All lanes : Anti-Cortactin antibody (ab230910) at 1/500 dilution
Lane 1 : HeLa (human epithelial cell line from cervix adenocarcinoma) treated with H2O2, whole cell extract
Lane 2 : HeLa treated with H2O2, whole cell extract with immunizing peptide
Predicted band size: 62 kDa
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab230910 has not yet been referenced specifically in any publications.