Key features and details
- Rabbit polyclonal to Cortactin
- Suitable for: IHC-P, WB
- Reacts with: Mouse
- Isotype: IgG
Product nameAnti-Cortactin antibody
See all Cortactin primary antibodies
DescriptionRabbit polyclonal to Cortactin
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse
Predicted to work with: Human
Recombinant fragment (His-T7-tag) corresponding to Mouse Cortactin aa 1-509. N-terminal tags. Expressed in E. coli.
MWKASAGHAVSITQDDGGADDWETDPDFVNDVSEKEQRWGAKTVQGSGHQ EHINIHKLRENVFQEHQTLKEKELETGPKASHGYGGKFGVEQDRMDRSAV GHEYQSKLSKHCSQVDSVRGFGGKFGVQMDRVDQSAVGFEYQGKTEKHAS QKDYSSGFGGKYGVQADRVDKSAVGFDYQGKTEKHESQKDYSKGFGGKYG IDKDKVDKSAVGFEYQGKTEKHESQKDYVKGFGGKFGVQTDRQDKCALGW DHQEKLQLHESQKDYKTGFGGKFGVQSERQDSSAVGFDYKERLAKHESQQ DYAKGFGGKYGVQKDRMDKNASTFEEVVQVPSAYQKTVPIEAVTSKTSNI RANFENLAKEREQEDRRKAEAERAQRMAKERQEQEEARRKLEEQARAKKQ TPPASPSPQPIEDRPPSSPIYEDAAPFKAEPSYRGSEPEPEYSIEAAGIP EAGSQQGLTYTSEPVYETTEAPGHYQAEDDTYDGYESDLGITAIALYDYQ AAGDDEISF
Database link: Q60598
- IHC-P: Mouse uterus and liver tissues. WB: Recombinant mouse Cortactin protein. Mouse brain, testis, pancreas and parotid gland tissue lysate.
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Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol
Concentration information loading...
PurityProtein A purified
Purification notesAntigen-specific affinity chromatography followed by Protein A affinity chromatography.
Alkaline Phosphatase detection kit
Our Abpromise guarantee covers the use of ab231620 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||Use a concentration of 5 - 20 µg/ml.|
|WB||Use a concentration of 0.5 - 2 µg/ml.|
FunctionContributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
Sequence similaritiesContains 7 cortactin repeats.
Contains 1 SH3 domain.
DomainThe SH3 motif may mediate binding to the cytoskeleton.
modificationsTyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
Cellular localizationCytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia.
- Information by UniProt
- Amplaxin antibody
- CTTN antibody
- EMS 1 antibody
Formalin-fixed, paraffin-embedded mouse uterus tissue stained for Cortactin with ab231620 at 20 µg/ml in immunohistochemical analysis. DAB staining.
All lanes : Anti-Cortactin antibody (ab231620) at 1 µg/ml
Lane 1 : Mouse testis tissue lysate
Lane 2 : Mouse brain tissue lysate
Lane 3 : Mouse pancreas tissue lysate
Lane 4 : Mouse parotid gland tissue lysate
All lanes : HRP-linked Guinea pig anti-Rabbit at 1/2000 dilution
Formalin-fixed, paraffin-embedded mouse liver tissue stained for Cortactin with ab231620 at 30 µg/ml in immunohistochemical analysis. DAB staining.
Anti-Cortactin antibody (ab231620) at 2 µg/ml + Recombinant mouse Cortactin protein
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab231620 has not yet been referenced specifically in any publications.