Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EP1922Y] to Cortactin (PE)
- Suitable for: Flow Cyt
- Conjugation: PE. Ex: 488nm, Em: 575nm
Product nameAnti-Cortactin antibody [EP1922Y] (PE)
See all Cortactin primary antibodies
DescriptionRabbit monoclonal [EP1922Y] to Cortactin (PE)
ConjugationPE. Ex: 488nm, Em: 575nm
Tested applicationsSuitable for: Flow Cytmore details
Predicted to work with: Mouse, Rat, Human
Synthetic peptide within Human Cortactin aa 1-100 (N terminal). The exact sequence is proprietary.
Database link: Q14247
- Flow Cyt: HeLa cells.
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at +4°C. Do Not Freeze. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 1% BSA, PBS
Concentration information loading...
PurityProtein A purified
- Anti-Cortactin antibody [EP1922Y] (Alexa Fluor® 488) (ab202648)
- Anti-Cortactin antibody [EP1922Y] (Alexa Fluor® 647) (ab202650)
- Anti-Cortactin antibody [EP1922Y] (Alexa Fluor® 555) (ab215298)
- Anti-Cortactin antibody [EP1922Y] (Alexa Fluor® 568) (ab215299)
- Anti-Cortactin antibody [EP1922Y] (Alexa Fluor® 594) (ab215300)
- Anti-Cortactin antibody [EP1922Y] - BSA and Azide free (ab223125)
- Anti-Cortactin antibody [EP1922Y] (ab81208)
Our Abpromise guarantee covers the use of ab209758 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
FunctionContributes to the organization of the actin cytoskeleton and cell structure. Plays a role in the regulation of cell migration. Plays a role in the invasiveness of cancer cells, and the formation of metastases.
Sequence similaritiesContains 7 cortactin repeats.
Contains 1 SH3 domain.
DomainThe SH3 motif may mediate binding to the cytoskeleton.
modificationsTyrosine phosphorylation in transformed cells may contribute to cellular growth regulation and transformation.
Cellular localizationCytoplasm > cytoskeleton. Cell projection > lamellipodium. Cell projection > ruffle. Associated with membrane ruffles and lamellipodia.
- Information by UniProt
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Overlay histogram showing HeLa cells stained with ab209758 (red line). The cells were fixed with 4% formaldehyde (10 min) and then permeabilized with 0.1% PBS-Triton X-100 for 15 min. The cells were then incubated in 1x PBS / 10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (ab209758, 1/5000 dilution) for 30 min at 22°C. Isotype control antibody (black line) was rabbit IgG (monoclonal) Phycoerythrin (ab209478) used at the same concentration and conditions as the primary antibody. Unlabelled sample (blue line) was also used as a control. Acquisition of >5,000 events were collected using a 50mW Yellow/Green laser (561nm) and 586/15 bandpass filter. This antibody gave a positive signal in HeLa cells fixed with 80% methanol (5 min)/permeabilized with 0.1% PBS-Triton X-100 for 15 min used under the same conditions.
ab209758 has not yet been referenced specifically in any publications.