Overview

  • Product name
    Corticosterone ELISA kit
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 5.3%
    Inter-assay
    Sample n Mean SD CV%
    Overall 10.6%
  • Sample type
    Cell culture supernatant, Saliva, Milk, Urine, Serum, Plasma
  • Assay type
    Competitive
  • Sensitivity
    = 0.28 ng/ml
  • Range
    2 ng/ml - 22 ng/ml
  • Recovery

    = 101 %

  • Assay time
    3h 00m
  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Mouse, Rat, Human, Baboon
  • Product overview

    Corticosterone in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit (ab108821) is designed for the quantitative measurement of Corticosterone levels in plasma, serum, urine, milk, saliva and cell culture supernatant.


    A Corticosterone specific antibody has been precoated onto 96-well plates and blocked. Standards or test samples are added to the wells and subsequently biotinylated Corticosterone is added and then followed by washing with wash buffer. Avidin-Biotin-Peroxidase Complex is added and unbound conjugates are washed away with wash buffer. TMB is then used to visualize HRP enzymatic reaction. TMB is catalyzed by HRP to produce a blue color product that changes into yellow after adding acidic stop solution. The density of yellow coloration is inversely proportional to the amount of Corticosterone captured in plate.

    Concentration of the kit components are lot-specific and the end user should always refer to the vial label

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at -20°C. Please refer to protocols.
  • Components 1 x 96 tests
    100X Streptavidin-Peroxidase Conjugate 1 x 80µl
    10X Diluent M Concentrate 1 x 20ml
    20X Wash Buffer Concentrate 1 x 30ml
    2X Biotinylated Corticosterone Protein (Lyophilized) 1 vial
    Chromogen Substrate 1 x 8ml
    Corticosterone Microplate (12 x 8 well strips) 1 unit
    Corticosterone Standard 1 vial
    Sealing Tapes 3 units
    Stop Solution 1 x 12ml
  • Research areas
  • Relevance
    Corticosterone is the adrenal steroid, the major glucocorticoid. Glucocorticoid hormones are also known as corticosteroid hormones and are synthesized mainly in the adrenal cortex; however, more recently the enzymes involved in their synthesis have been found in a variety of cells and tissues, including the heart. The effects of these hormones are mediated via both cytoplasmic mineralocorticoid receptors (MRs) and glucocorticoid receptors (GRs), which act as ligand-inducible transcription factor. Corticosterone has profound effect on the structure and function of the hippocampus. Brain corticosterone action through the glucocorticoid receptor may involve memory storage. Emotional stress might cause increases in plasma corticosterone.

Applications

Our Abpromise guarantee covers the use of ab108821 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Competitive ELISA with ab108821.
  • Corticosterone measured in biological fluids showing quantity (ng) per mL of tested sample

Protocols

References

This product has been referenced in:
  • Dumaine JE & Ashley NT Acute sleep fragmentation does not alter pro-inflammatory cytokine gene expression in brain or peripheral tissues of leptin-deficient mice. PeerJ 6:e4423 (2018). Read more (PubMed: 29479505) »
  • Kinyua AW  et al. Insulin Regulates Adrenal Steroidogenesis by Stabilizing SF-1 Activity. Sci Rep 8:5025 (2018). Read more (PubMed: 29567944) »
See all 33 Publications for this product

Customer reviews and Q&As

1-10 of 20 Abreviews or Q&A

Answer

Unfortunately the components of this buffer are considered proprietary. And while this buffer has not been tested for use with protein assays, its likley that some of the components would cause erroneous results in a protein assay.

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Answer



It is always good idea to run creatinine assay as a normalization factor for the urine sample since the urine concentration is varied significantly. Some of our customers do use a creatinine assay but others don't so it is really up to you.

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J’ai effectué une extraction du corticostérone de fèces provenant de babouins Papio Anubis selon le protocole décrit dans l’article suivant « Monkeys in the Middle : Parasite Transmission through the Social Network of a Wild Primate » , Andrew J.J. Maclntosh,2012.
(J’ai cuis les fèces dans un four à 60° pendant 3h pour 1 g de fèces).
Ensuite j’ai utilisé le kit ab108821 de abcam pour doser la corticostérone.
J’ai testé différentes dilutions et il en sort qu’on peut utiliser l’échantillon non dilué.
J’ai obtenu des résultats qui correspondent au moyenne à une concentration de 4.52 ng/ml.
Par contre au niveau des contrôles j’ai obtenu des résultats que je ne comprends pas :
-Dans le premier contrôle je mets tout saut l’Ag et j’obtiens un très forte Abs, ce qui correspond à une très faible concentration – c’est normal.
-Dans le 2ème contrôle je mets tout sauf le 2ème AcI (l’Ag utilisé est celui correspondant au point 1 de la courbe standard [100ng/ml]) et là j’obtient une Abs proche d’une Abs correspondant à la concentration [100ng/ml]).
-Dans un 3ème contrôle je mets tout sauf l’AcII (l’Ag utilisé est celui correspondant au point 1 de la courbe standard [100ng/ml]) et là j’obtiens une Abs très faible correspondant à une concentration très forte.
-Dans un 4ème contrôle je mets uniquement du diluant et là j’obtiens une Abs très faible correspondant à une concentration très forte comme dans le contrôle précédent.

Corticosterone was extracted from feces of Papio Anubis baboons according to the protocol described in the following article : « Monkeys in the Middle : Parasite Transmission through the Social Network of a Wild Primate » , Andrew J.J. Maclntosh,2012.
(I cook feces in an oven at 60 ° for 3 h per 1 g of feces).
Then I used the kit ab108821 from Abcam to quantify the corticosterone.
I tested different dilutions and found out undiluted sample can be used.
I determined an average concentration of 4.52 ng/ml.
However in the controls I obtained results I do not understand:
- In the first test I put all jump Ag and I get a very strong Abs, which corresponds to a very low concentration - this is normal.
- In the second control I put everything except the second ACI (Ag used is the one corresponding to the point of the standard curve [100ng/ml]) and then I get a close Abs Abs corresponding to a concentration [100ng/ml]).
- In a third control I put everything except the ACII (Ag used is the one corresponding to the point of the standard curve [100ng/ml]) and then I get a very low Abs corresponding to a very high concentration.
- In a fourth-control I put only the diluent and then I get a very low Abs corresponding to a high concentration as in the previous test.

Abcam user community

Verified customer

Submitted Jun 18 2013

Question
Answer

Yes. This kit is a competitive ELISA assay. Corticostone is competing with the biotinylated-corticosterone. The higher the concentration of corticosterone in sample, the lower OD.

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Answer

Thank you for contacting us.

According to the protocol on our datasheet, this kit contains sealing tapes: Each kit contains 3 pre-cut, pressure-sensitive sealing tapes that can be cut to fit the format of the individual assay.

Regarding the storage, the protocol states the following: "ab108821 may be stored until the expiration date at 2 to 8°C or -20°C from the date of shipment. Store SP Conjugate at -20°C upon arrival up to the expiration date. Store Microplate, Diluent Concentrate (10x), Wash Buffer, Stop Solution, and Chromogen Substrate at 2-8°C. Diluent (1x) may be stored for up to 1 month at 2-8°C. Store Standard and Biotinylated Protein at 2-8°C before reconstituting with Diluent and at -20°C after reconstituting with
Diluent. Opened unused microplate wells may be returned to the foil pouch with the desiccant packs. Reseal along zip-seal. May be stored for up to 1 month in a vacuum desiccator.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question
Answer

Thank you for contacting us. Since Corticosterone is stable molecule, we think it should work. But there is no guarantee since we have not tested this condition.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

La source du kit m'a informé qu'ils n'avaient malheureusement jamais testé ce genre de kits sur des selles. Leur recommandation est d'essayer une série de dilutions au 1:2, 1:4, 1:8, etc.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

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Answer

Code de remise : ****
Date d’expiration : jj mm aaaa

Je suis ravi que vous souhaitiez participer à notre offre et tester ab108821 sur selles de singe.
Le code de réduction correspondant est ****. Ce code de réduction est utilisable sous 120 jours et pour l’achat d’un autre produit abcam. Inutile de mentionner ce code sur votre première commande.

Pour activer ce code, vous devez dans un premier temps soumettre une Abreview avec vos résultats chez le singe et noter ce code dans la section « Additional notes ». Ainsi activé, ce code vous donnera une réduction sur votre prochaine commande de la valeur du ab108821. Veuillez contacter notre service clientèle en vous munissant des informations suivantes : code de réduction, numéro de commande de ab108821.

Je vais demander au laboratoire si ils ont des recommandations pour la préparation des échantillons. Je vous contacterai de nouveau quand j'aurai reçu une réponse. Merci pour votre patience.

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Answer

Thank you for contacting us.

Unfortunately, this kit has not been tested for cross-reactivity with 11-DHC, so we do not have any information about whether or not it would cross-react. I am sorry I could not be more helpful, please let me know if you have any additional questions.

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Answer

Thank you for contacting us.

In order to determine the sample concentration values use the standard curve equation. Substitute the values for the absorbances obtained in the equation and multiply the value by the dilution factor.

Check the values are within the normal range for your sample type.

I hope this helps. Please do not hesitate to contact us for further assistance.

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1-10 of 20 Abreviews or Q&A

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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