Overview

  • Product name
    Anti-Corticotropin Releasing Factor antibody
    See all Corticotropin Releasing Factor primary antibodies
  • Description
    Rabbit polyclonal to Corticotropin Releasing Factor
  • Host species
    Rabbit
  • Tested applications
    Suitable for: RIA, IHC-Pmore details
  • Species reactivity
    Reacts with: Rat, Human
  • Immunogen

    Synthetic peptide within Rat Corticotropin Releasing Factor aa 1-41. The exact sequence is proprietary.
    Database link: P01143

  • Positive control
    • IHC-P: Rat brain tissue; Human placenta tissue.

Properties

Applications

Our Abpromise guarantee covers the use of ab8901 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA 1/50000.
IHC-P Use at an assay dependent concentration.

Target

  • Relevance
    CRF precursor is a 41-amino acid peptide derived from a 196-amino acid preprohormone. CRF is a neuroendocrine hormone secreted by the paraventricular nucleus (PVN) of the hypothalamus and is a key player in the hypothalamic pituitary adrenal axis. It is also synthesized in peripheral tissues, such as T lymphocytes and is highly expressed in the placenta. Neuronal CRF is important in a variety of diverse roles including, memory, depression, stress, kidney function, parturition, metabolism and feeding (to mention a few).
  • Cellular localization
    Cytoplasmic and Secreted
  • Database links
  • Alternative names
    • Corticoliberin antibody
    • Corticotropin releasing factor antibody
    • Corticotropin releasing hormone antibody
    • CRF antibody
    • Crh antibody
    • CRH1 antibody
    see all

Images

  • Ab8901 staining Corticotropin releasing factor in Human placenta tissue sections by Immunohistochemistry (IHC-P - paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 2% BSA for 10 minutes at 21°C; antigen retrieval was by heat mediation in a citrate buffer. Samples were incubated with primary antibody (1/4000 in blocking buffer) for 16 hours at 21°C. A diluted Biotin-conjugated Goat anti-rabbit IgG polyclonal (1/300) was used as the secondary antibody.

    See Abreview

  • Immunohistochemical analysis of rat brain tissue, staining Corticotropin Releasing Factor with ab8901.

    Tissue was fixed with paraformaldehyde, permeabilized with 0.01% Triton X-100 and blocked with 10% serum for 1 hour at 25°C; antigen retrieval was by heat mediation in a citrate buffer (pH 6). Samples were incubated with primary antibody (1/100 in diluent) for 24 hours at 4°C. An AlexaFluor®594-conjugated goat anti-rabbit polyclonal IgG (1/500) was used as the secondary antibody. The figure on the left is a secondary only control.

    See Abreview

  • Radioimmunoassay using ab8901
    - Day 1: Add Recombinant Protein A/G (10ug/ml) to a 96 wells plate; parafilm wrap; incubate overnight at 4°C.
    - Day 2: Aspirate and wash the plate to remove loosely bound Protein A/G; add antibody solution (dilution 1:50000); parafilm wrap; incubate 24h at 4°C.
    - Day 3: Aspirate and wash the plate to remove loosely bound antibody; add CRF standard (0, 0.03, 0.1, 0.3, 1, 3, 10, 30 ng/ml); parafilm wrap; incubate 24h at 4°C.
    - Day 4: DO NOT ASPIRATE; add 125I-CRF; incubate 24h at 4°C.
    - Day 5: Aspirate and wash the plate; break the plate and collect the wells in scintillation tubes; add 2ml of scintillation liquid; count in beta counter (open the 3H window to at least 30-40 keV), at least 2min/tube.

References

This product has been referenced in:
  • Trifunovic S  et al. Histological and morphofunctional parameters of the hypothalamic-pituitary-adrenal system are sensitive to daidzein treatment in the adult rat. Acta Histochem 120:129-135 (2018). IHC-P . Read more (PubMed: 29338944) »
  • Zhu J  et al. Electroacupuncture Alleviates Surgical Trauma-Induced Hypothalamus Pituitary Adrenal Axis Hyperactivity Via microRNA-142. Front Mol Neurosci 10:308 (2017). Read more (PubMed: 29021740) »
See all 10 Publications for this product

Customer reviews and Q&As

1-10 of 14 Abreviews or Q&A

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Human Tissue sections (Placenta)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citric acid
Permeabilization
No
Specification
Placenta
Blocking step
BSA as blocking agent for 10 minute(s) · Concentration: 2% · Temperature: 21°C
Fixative
Formaldehyde

Mr. Carl Hobbs

Verified customer

Submitted Mar 27 2017

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Non-reduced Denaturing
Sample
Mouse Tissue lysate - whole (Hypothalamus)
Specification
Hypothalamus
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 25°C

Mr. Jean-Christophe Helbling

Verified customer

Submitted Sep 25 2013

Abcam guarantees this product to work in the species/application used in this Abreview.
Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Brain)
Specification
Brain
Fixative
Paraformaldehyde
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: Citrate
Permeabilization
Yes - 0.01% Triton-X
Blocking step
Serum as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 10% · Temperature: 25°C

Abcam user community

Verified customer

Submitted Jan 04 2013

Answer

Thank you for sending us your customer's questionnaire and for contacting us. I am sorry to hear this antibody is not providing satisfactory results.

The details provided will enable us to investigate this case and will provide us with vital information for monitoring product quality. It is regrettable the results have not been successful.

Having reviewed the protocol details, I believe this product should have given satisfactory results.
I apologize for the inconvenience and am pleased to offer you a free of charge replacement, credit note, or refund in compensation. It seems as though your customer would prefer a replacement antibody, so please let me know the catalogue number and I will arrange that for you.

Thank you for your cooperation. I look forward to hearing from you with details of how you would like to proceed.

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Question
Answer

Thank you for contacting us.


The link to our conjugation kits and purifications kits is either https://www.abcam.com/EasyLink or
https://www.abcam.com/index.html?pageconfig=resource&rid=13148&source=pagetrap&viapagetrap=easylink

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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Answer

Thank you for your reply. I have published your Abreview on our online datasheet and awarded Abpoints to your user account. You can view and redeem your Abpoints by logging in to your account on our website.

Additionally, since you have submitted an image of an application that did not previously have an image, the discount code *** will allow you to receive a free primary antibody on your next purchase from us before September 2nd, 2012. This code can be used by anyone in your institution, and the order can be placed by phone, fax, email, or online.

I hope this helps, please let me know if you need any other information or assistance.

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Question
Answer

Thank you for contacting Abcam regarding ab8901.


The predicted molecular weight of this protein is 21kDa. In WB you should detect a band at approximately this size.


I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

Read More

Answer

I am very pleased to hear you would like to use ab8901 in RIA and send us your data and an image of your results.

This code will give you 1 freeprimary antibody before the expiration date. To redeem this offer, please submit an Abreview with the image for RIA and include this code in the Additional Comments section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please read the following:

Typically, researchers can submit a product review via an online submission form. At this time we do not have an Abreview form available for submitting reviews on this product. As an alternative, please email your review of ab8901 to me.

In the review, please include:
1) A brief description of your experimental procedures
2) An attached JPEG or PNG formatted image illustrating your data
2) Your feelings on the results
3) If you would like your name to be shown with your review or if you prefer to be anonymous

Regarding your image, please note any descriptive details that will help other researchers interpret your results.

You will be awarded the same number of points as if you had submitted the review online using a standard Abreview form. You will receive 120 points for text with an additional 100 points for including an image. These points can be used for discounts on future purchases or Amazon gift cards.

The code will be active once the Abreview with the image has been submitted and can be redeemed in one of the following ways:
1) Call to place your order and mention the code to our customer service department;
2) Include the code in your fax order;
3) Place your order on the web and enter the promotional code.

If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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Answer

Thank you for your reply.

I do apologize as I have been unable to obtain any more specific information regarding the dilution of this antibody to be used in RIA.

Due to this lack of specific details, I would like to offer you a testing discount, whereby you would purchase the antibody and test it in RIA and then submit your protocol and data to me which would be published on our website as an Abreview. Once that review has been received, you would be eligible to receive a free primary antibody on a future order.


Plesae let me know if this would be of interest to you and I will issue you a discount code. I do apologize for any inconvenience caused. I look forward to your reply so that I may assist you further.

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Answer

Thank you for contacting Abcam regarding ab8901.


I have confirmed with the laboratory that RIA was not tested in house, but based on the following references:

1. Widmaier EP, Lim AT, Vale W. (1989) Secretion of corticotropin-releasing factor from cultured rat hypothalamic cells: effects of catecholamines. Endocrinol 124:583-590.

2. Vale W, Vaughan J, Yamamoto G, Bruhn T, Douglas C, Dalton D, Rivier C, Rivier J. (1983) Assay of corticotopin releasing factor. Meth Enzymol 103:565-577.

I hope this information is helpful. Please do not hesitate to contact us if you have any additional questions.

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1-10 of 14 Abreviews or Q&A

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