Overview

  • Product name
    Anti-Cortisol antibody [CORT-2]
    See all Cortisol primary antibodies
  • Description
    Mouse monoclonal [CORT-2] to Cortisol
  • Host species
    Mouse
  • Specificity
    Cortisol-BSA conjugate and free cortisol. There is no cross-reactivity with BSA.
  • Tested applications
    Suitable for: competitive binding assaysmore details
  • Species reactivity
    Reacts with: Human
  • Immunogen

    Recombinant full length protein (Human) conjugated to BSA.

  • General notes
    Concentration varies from lot to lot and can be provided on request.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.1% Sodium Azide
    Constituents: PBS, pH 7.4
  • Concentration information loading...
  • Purity
    Protein G purified
  • Purification notes
    Purity tested by elcetrophoresis.
  • Clonality
    Monoclonal
  • Clone number
    CORT-2
  • Myeloma
    Sp2/0
  • Isotype
    IgG3
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1952 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
competitive binding assays Use at an assay dependent dilution.

Target

  • Relevance
    Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.
  • Cellular localization
    Secreted

Images

  • Cross-reactivity, % 50% displacement of cortisol-3-125-Iodine.

References

This product has been referenced in:
  • Chen SR  et al. Disruption of genital ridge development causes aberrant primordial germ cell proliferation but does not affect their directional migration. BMC Biol 11:22 (2013). IHC . Read more (PubMed: 23497137) »
  • Qian X  et al. Palladin is a regulator of actin filament bundles at the ectoplasmic specialization in adult rat testes. Endocrinology 154:1907-20 (2013). Read more (PubMed: 23546604) »
See all 3 Publications for this product

Customer reviews and Q&As

1-2 of 2 Abreviews or Q&A

Answer

Thank you for contacting us.

To be honest we do not have an antibody that we have tested with this kind of novel assay, and therefore do not know if it would work nor can we guarantee it.

Although ab1949 may work, I may recommend ab1952: It is also a mouse monoclonal, is guaranteed to work in competitive binding assays (which might be more related to the intended experimental approach) and crucially detects also free cortisol. Furthermore, there is no cross-reactivity with cortisone, if this is of importance to the customer. (ab1951 is similar, but it cross-reacts with cortisone).

https://www.abcam.com/index.html?datasheet=1952 (or use the following: https://www.abcam.com/index.html?datasheet=1952).

https://www.abcam.com/index.html?datasheet=1951 (or use the following: https://www.abcam.com/index.html?datasheet=1951).

If the customer considers testing this antibody in this application though, we would be very grateful to receive some feedback (probably once the results are published).

Also, we unfortunately do not have Cortisol-3-CMO-NHS ester or something similar available in our catalogue at the moment.

I am sorry that I cannot be of more assistance on this occasion, but I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Answer

Cross-reaction of cortisol antibodies with cortisone is the following: ab1949 - 0.6 %, ab1951 - 35 % and ab1952 - 0 %.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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