Overview

  • Product name

    Anti-Cortisol antibody [F4P1A3]
    See all Cortisol primary antibodies
  • Description

    Mouse monoclonal [F4P1A3] to Cortisol
  • Host species

    Mouse
  • Tested applications

    Suitable for: IP, ELISA, RIAmore details
  • Immunogen

    Cortisol-3-CMO conjugated to BSA.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at 4°C (up to 6 months). Store at -20°C long term.
  • Storage buffer

    Preservative: 0.05% Sodium azide
    Constituents: 0.019% Potassium chloride, 0.79% Sodium chloride, 0.14% Potassium phosphate, 0.1% BSA, 50% Glycerol
  • Concentration information loading...
  • Purity

    Protein A purified
  • Clonality

    Monoclonal
  • Clone number

    F4P1A3
  • Isotype

    IgG2b
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab116600 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
IP Use at an assay dependent dilution.
ELISA Use at an assay dependent dilution.
RIA Use at an assay dependent dilution.

Target

  • Relevance

    Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.
  • Cellular localization

    Secreted

References

ab116600 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

Answer

Thank you for contacting us and your interest in our products.

We have two antibodies directed against cortisol which have been used in ELISA previously. These are the mouse monolonals ab1949 and ab116600. However these have not been used in a sandwich ELISA format or "diurnal cortisol assay and subsequently a cross reactant assay" as you have described and we would therefore not be able to guarantee that they would be suitable for this experiment. If you could describe in a bit more detail how you intend to use the antibody I may be able to comment further on whether these antibodies are likely to work in this setting.

Alternatively we have ELISA kits designed to detect cortisol in human serum or plasma (ab108665), urine (ab108674) or saliva (ab154996) which may be of interest to you. These are based on a competitive immunoenzymatic colorimetric method intended for the quantitative determination of Cortisol.

I hope this information has been helpful. If I can be of any further assistance, please do not hesitate to let me know.

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