Anti-Cortisol antibody [XM210] (ab1949)

Submit a review Q&A (6)References (11)

Key features and details

  • Mouse monoclonal [XM210] to Cortisol
  • Suitable for: ELISA
  • Isotype: IgG2a

Overview

  • Product name

    Anti-Cortisol antibody [XM210]
    See all Cortisol primary antibodies

  • Description

    Mouse monoclonal [XM210] to Cortisol

  • Host species

    Mouse

  • Specificity

    Cortisol, Ka = 1.7 x 109 M-1. XM-210 has some cross-reactivity with cortisone (~0.6%)

  • Tested applications

    Suitable for: ELISAmore details

  • Immunogen

    Chemical/ Small Molecule corresponding to Cortisol conjugated to Bovine Serum Albumin (BSA).

  • General notes

    Concentration varies from lot to lot and can be provided on request.

Properties

  • Form

    Liquid

  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.

  • Storage buffer

    pH: 7.50
    Preservative: 0.05% Sodium azide
    Constituents: 0.121% Tris, 0.87% Sodium chloride
  • Concentration information loading...

  • Purity

    Protein A purified

  • Purification notes

    Purified by ion exchange chromatography and tested by electrophoresis.

  • Clonality

    Monoclonal

  • Clone number

    XM210

  • Myeloma

    Sp2/0

  • Isotype

    IgG2a

  • Research areas

Applications

The Abpromise guarantee

Our Abpromise guarantee covers the use of ab1949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA
Use at an assay dependent concentration.
Notes
ELISA
Use at an assay dependent concentration.

Target

  • Relevance

    Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.

  • Cellular localization

    Secreted

Protocols

To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.

Click here to view the general protocols

References (11)

Publishing research using ab1949? Please let us know so that we can cite the reference in this datasheet.

ab1949 has been referenced in 11 publications.

  • Shin J  et al. Smart Fatigue Phone: Real-time estimation of driver fatigue using smartphone-based cortisol detection. Biosens Bioelectron 136:106-111 (2019). PubMed: 31048212
  • Mes D  et al. Neurobiology of Wild and Hatchery-Reared Atlantic Salmon: How Nurture Drives Neuroplasticity. Front Behav Neurosci 12:210 (2018). PubMed: 30254575
  • Höglund E  et al. Dietary l-tryptophan leaves a lasting impression on the brain and the stress response. Br J Nutr 117:1351-1357 (2017). PubMed: 28625179
  • van den Bos R  et al. Further characterisation of differences between TL and AB zebrafish (Danio rerio): Gene expression, physiology and behaviour at day 5 of the larval stage. PLoS One 12:e0175420 (2017). RIA . PubMed: 28419104
  • Munje RD  et al. A new paradigm in sweat based wearable diagnostics biosensors using Room Temperature Ionic Liquids (RTILs). Sci Rep 7:1950 (2017). PubMed: 28512341
View all Publications for this product

Customer reviews and Q&As

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1-6 of 6 Abreviews or Q&A

Question

Are these antibodies cross reactive with dog cortisol and testosterone?

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Answer

To our knowledge, ab1949 nor ab6066 have not yet been tested in detecting canine cortisol and testosterone. However, as cortisol and testosterone are steroids, antibodies raised against them should detect them regardless of the species that they are produced in as their structure and composition should be the same.

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Question

Inquiry: Hi I require an anti-cortisol antibody which I can use to coat ELISA plates for both a diurnal cortisol assay and subsequently a cross reactant assay. The reaction will be detected by either OPD or TMB. Are any of your anti-cortisol antibodies suitable and which is the best

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Answer

Thank you for contacting us and your interest in our products.

We have two antibodies directed against cortisol which have been used in ELISA previously. These are the mouse monolonals ab1949 and ab116600. However these have not been used in a sandwich ELISA format or "diurnal cortisol assay and subsequently a cross reactant assay" as you have described and we would therefore not be able to guarantee that they would be suitable for this experiment. If you could describe in a bit more detail how you intend to use the antibody I may be able to comment further on whether these antibodies are likely to work in this setting.

Alternatively we have ELISA kits designed to detect cortisol in human serum or plasma (ab108665), urine (ab108674) or saliva (ab154996) which may be of interest to you. These are based on a competitive immunoenzymatic colorimetric method intended for the quantitative determination of Cortisol.

I hope this information has been helpful. If I can be of any further assistance, please do not hesitate to let me know.

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Question

Hi,

Thank you for your email.

Sorry our customer and I forgot to mentioned ELISA as the application.

Let me know if you need any further info.

Kind regards,

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Answer

Thank you for confirming that information.

The antibodies you have suggested for DHEA (ab54202, ab35062 and ab123987) should be fine for what the customer has requested.

The same is true of the anti-DHEA-S (ab31668). The anti-Cortisol antibodies ab1949 and ab116600 can also be suggested. The anti-Androstendione you have suggested should also be suitable (ab34944).

We do also have an antibody raised to target the hydroxy-Pregnenolone (ab34916). I am not sure if this is what the customer is after but it may be worth them having a look.

The secondary antibody they need would depend on which antibodies they choose to buy in the end. We ahve a very good search engine which you can use to find suitable antibodies:

https://www.abcam.com/index.html?pageconfig=productmap&cl=918

All you need to do is put in the isotype of the primary antibody and the species they are raised in (available on the datasheet of the primary antibody), specify that it is to be used in ELISA and that it is an HRP conjugate you want.

If you want further help with this, please let me know which antibodies the customer decides to try.

I hope this information has been of help. If I can be of any further assistance, please do not hesitate to contact me again.

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Question

Our customer is looking forcortisol antibody and I have attached reference.

I searched one ofcortisol antibody from abcam website.
https://www.abcam.com/Cortisol-antibody-XM210-ab1949.html

She is going to follow this test so I also searched Cortisol 3(CMO).

Please let me know if ab1949 is okay for this experiment.
Best regards

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Answer

Thank you for contacting us.

To be honest we do not have an antibody that we have tested with this kind of novel assay, and therefore do not know if it would work nor can we guarantee it.

Although ab1949 may work, I may recommend ab1952: It is also a mouse monoclonal, is guaranteed to work in competitive binding assays (which might be more related to the intended experimental approach) and crucially detects also free cortisol. Furthermore, there is no cross-reactivity with cortisone, if this is of importance to the customer. (ab1951 is similar, but it cross-reacts with cortisone).

https://www.abcam.com/Cortisol-antibody-CORT-2-ab1952.html (or use the following: https://www.abcam.com/Cortisol-antibody-CORT-2-ab1952.html).

https://www.abcam.com/Cortisol-antibody-CORT-1-ab1951.html (or use the following: https://www.abcam.com/Cortisol-antibody-CORT-1-ab1951.html).

If the customer considers testing this antibody in this application though, we would be very grateful to receive some feedback (probably once the results are published).

Also, we unfortunately do not have Cortisol-3-CMO-NHS ester or something similar available in our catalogue at the moment.

I am sorry that I cannot be of more assistance on this occasion, but I hope this information is nevertheless helpful to you. Please do not hesitate to contact us if you need any more advice or information.

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Question

Email: yvonne.couch@pharm.ox.ac.uk I would like to run a cortisol/hydrocortisone ELISA on mouse serum, does this product react with mouse serum?

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Answer

Thanks for your inquiry. Yes ab1949 should work in mouse serum. However, there will likely be an issue with non-specific binding of your secondary antibody, which will be an anti-mouse IgG, with the IgG in the mouse serum. Of course, this is assuming you are not doing a direct ELISA, which is probably the best way to test your sample to eliminate the secondary antibody issues. I hope this answers your question. Please feel free to contact me if you require further assistance.

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Question

I think that I asked the wrong question. At what position is BSA attached to the immunogen? Thank you

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Answer

Thank you for your enquiry. The immunogen is Cortisol-3(CMO)-BSA. Please do not hesitate to contact us if you need anything further.

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