Overview

  • Product name
    Anti-Cortisol antibody [XM210]
    See all Cortisol primary antibodies
  • Description
    Mouse monoclonal [XM210] to Cortisol
  • Host species
    Mouse
  • Specificity
    Cortisol, Ka = 1.7 x 109 M-1. XM-210 has some cross-reactivity with cortisone (~0.6%)
  • Tested applications
    Suitable for: RIA, ELISAmore details
  • Immunogen

    Chemical/ Small Molecule corresponding to Cortisol conjugated to Bovine Serum Albumin (BSA).

  • General notes
    Concentration varies from lot to lot and can be provided on request.

Properties

  • Form
    Liquid
  • Storage instructions
    Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
  • Storage buffer
    Preservative: 0.05% Sodium Azide
    Constituents: 0.15M Sodium Chloride, 10mM Tris. pH 7.5
  • Concentration information loading...
  • Purity
    Protein A purified
  • Purification notes
    Purified by ion exchange chromatography and tested by elctrophoresis.
  • Clonality
    Monoclonal
  • Clone number
    XM210
  • Myeloma
    Sp2/0
  • Isotype
    IgG2a
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab1949 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
RIA Use at an assay dependent concentration. PubMed: 23077165
ELISA Use at an assay dependent concentration.

Target

  • Relevance
    Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism.
  • Cellular localization
    Secreted

References

This product has been referenced in:
  • van den Bos R  et al. Further characterisation of differences between TL and AB zebrafish (Danio rerio): Gene expression, physiology and behaviour at day 5 of the larval stage. PLoS One 12:e0175420 (2017). RIA . Read more (PubMed: 28419104) »
  • Munje RD  et al. A new paradigm in sweat based wearable diagnostics biosensors using Room Temperature Ionic Liquids (RTILs). Sci Rep 7:1950 (2017). Read more (PubMed: 28512341) »

See all 7 Publications for this product

Publishing research using ab1949? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

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6 reviews or Q&A

10
Votes: Highest First

Are these antibodies cross reactive with dog cortisol and testosterone?

To our knowledge, ab1949 nor ab6066 have not yet been tested in detecting canine cortisol and testosterone. However, as cortisol and testosterone are steroids, antibodies raised against them should detect them regardless of the species that they are pr...

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Inquiry: Hi I require an anti-cortisol antibody which I can use to coat ELISA plates for both a diurnal cortisol assay and subsequently a cross reactant assay. The reaction will be detected by either OPD or TMB. Are any of your anti-cortisol antibodies...

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Thank you for contacting us and your interest in our products.

We have two antibodies directed against cortisol which have been used in ELISA previously. These are the mouse monolonals ab1949 and ab116600. However these have not been used in a...

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Hi,

Thank you for your email.

Sorry our customer and I forgot to mentioned ELISA as the application.

Let me know if you need any further info.

Kind regards,

Thank you for confirming that information.

The antibodies you have suggested for DHEA (ab54202, ab35062 and ab123987) should be fine for what the customer has requested.

The same is true of the anti-DHEA-S (ab31668). The anti-Cortis...

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Our customer is looking forcortisol antibody and I have attached reference.

I searched one ofcortisol antibody from abcam website.
https://www.abcam.com/Cortisol-antibody-XM210-ab1949.html

She is going to follow this test so ...

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Thank you for contacting us.

To be honest we do not have an antibody that we have tested with this kind of novel assay, and therefore do not know if it would work nor can we guarantee it.

Although ab1949 may work, I may recommend ab...

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Email: yvonne.couch@pharm.ox.ac.uk I would like to run a cortisol/hydrocortisone ELISA on mouse serum, does this product react with mouse serum?

Thanks for your inquiry. Yes ab1949 should work in mouse serum. However, there will likely be an issue with non-specific binding of your secondary antibody, which will be an anti-mouse IgG, with the IgG in the mouse serum. Of course, this is assu...

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I think that I asked the wrong question. At what position is BSA attached to the immunogen? Thank you

Thank you for your enquiry. The immunogen is Cortisol-3(CMO)-BSA. Please do not hesitate to contact us if you need anything further.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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