Anti-Cortisol antibody [XM210] (ab1949)
Key features and details
- Mouse monoclonal [XM210] to Cortisol
- Suitable for: ELISA
- Isotype: IgG2a
Overview
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Product name
Anti-Cortisol antibody [XM210]
See all Cortisol primary antibodies -
Description
Mouse monoclonal [XM210] to Cortisol -
Host species
Mouse -
Specificity
Cortisol, Ka = 1.7 x 109 M-1. XM-210 has some cross-reactivity with cortisone (~0.6%)
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Tested applications
Suitable for: ELISAmore details -
Immunogen
Chemical/ Small Molecule corresponding to Cortisol conjugated to Bovine Serum Albumin (BSA).
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General notes
Concentration varies from lot to lot and can be provided on request.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.50
Preservative: 0.05% Sodium azide
Constituents: 0.121% Tris, 0.87% Sodium chloride -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified by ion exchange chromatography and tested by electrophoresis. -
Clonality
Monoclonal -
Clone number
XM210 -
Myeloma
Sp2/0 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism. -
Cellular localization
Secreted
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
References (11)
ab1949 has been referenced in 11 publications.
- Shin J et al. Smart Fatigue Phone: Real-time estimation of driver fatigue using smartphone-based cortisol detection. Biosens Bioelectron 136:106-111 (2019). PubMed: 31048212
- Mes D et al. Neurobiology of Wild and Hatchery-Reared Atlantic Salmon: How Nurture Drives Neuroplasticity. Front Behav Neurosci 12:210 (2018). PubMed: 30254575
- Höglund E et al. Dietary l-tryptophan leaves a lasting impression on the brain and the stress response. Br J Nutr 117:1351-1357 (2017). PubMed: 28625179
- van den Bos R et al. Further characterisation of differences between TL and AB zebrafish (Danio rerio): Gene expression, physiology and behaviour at day 5 of the larval stage. PLoS One 12:e0175420 (2017). RIA . PubMed: 28419104
- Munje RD et al. A new paradigm in sweat based wearable diagnostics biosensors using Room Temperature Ionic Liquids (RTILs). Sci Rep 7:1950 (2017). PubMed: 28512341