Anti-Cortisol antibody [XM210] (ab1949)
Key features and details
- Mouse monoclonal [XM210] to Cortisol
- Suitable for: ELISA
- Reacts with: Species independent
- Isotype: IgG2a
Overview
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Product name
Anti-Cortisol antibody [XM210]
See all Cortisol primary antibodies -
Description
Mouse monoclonal [XM210] to Cortisol -
Host species
Mouse -
Specificity
Cortisol, Ka = 1.7 x 109 M-1. XM-210 has some cross-reactivity with cortisone (~0.6%)
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Tested applications
Suitable for: ELISAmore details -
Species reactivity
Reacts with: Species independent -
Immunogen
Chemical/ Small Molecule corresponding to Cortisol conjugated to Bovine Serum Albumin (BSA).
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General notes
Concentration varies from lot to lot and can be provided on request.The Life Science industry has been in the grips of a reproducibility crisis for a number of years. Abcam is leading the way in addressing this with our range of recombinant monoclonal antibodies and knockout edited cell lines for gold-standard validation. Please check that this product meets your needs before purchasing.
If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, along with publications, customer reviews and Q&As
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.40
Preservative: 0.09% Sodium azide
Constituent: PBS -
Concentration information loading...
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Purity
Protein A purified -
Purification notes
Purified by ion exchange chromatography and tested by electrophoresis. -
Clonality
Monoclonal -
Clone number
XM210 -
Myeloma
Sp2/0 -
Isotype
IgG2a -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab1949 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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ELISA |
Use at an assay dependent concentration.
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Notes |
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ELISA
Use at an assay dependent concentration. |
Target
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Relevance
Cortisol is the most potent glucocorticoid produced by the human adrenal. It is synthesized from cholesterol and its production is stimulated by pituitary adrenocorticotropic hormone (ACTH) which is regulated by corticotropin releasing factor (CRF). ACTH and CRF secretions are inhibited by high cortisol levels in a negative feedback loop. In plasma a majority of cortisol is bound with high affinity to corticosteroid binding globulin (CBG or transcotin). Cortisol acts through specific intracellular receptors and affects numerous physiologic systems including immune function, glucose counter regulation, vascular tone, and bone metabolism. -
Cellular localization
Secreted
Protocols
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
Datasheets and documents
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SDS download
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Datasheet download
References (17)
ab1949 has been referenced in 17 publications.
- Park JH et al. Normalizing the Optical Signal Enables Robust Assays with Lateral Flow Biosensors. ACS Omega 7:17723-17731 (2022). PubMed: 35664567
- Koyasu H et al. Correlations between behavior and hormone concentrations or gut microbiome imply that domestic cats (Felis silvestris catus) living in a group are not like 'groupmates'. PLoS One 17:e0269589 (2022). PubMed: 35895662
- Koyasu H et al. Sociality of Cats toward Humans Can Be Influenced by Hormonal and Socio-Environmental Factors: Pilot Study. Animals (Basel) 13:N/A (2022). PubMed: 36611754
- Höglund E et al. Neuroendocrine indicators of allostatic load reveal the impact of environmental acidification in fish. Comp Biochem Physiol C Toxicol Pharmacol 229:108679 (2020). PubMed: 31794875
- Laberge F et al. Temporal profiles of cortisol accumulation and clearance support scale cortisol content as an indicator of chronic stress in fish. Conserv Physiol 7:coz052 (2019). PubMed: 31620290