Overview

  • Product name

    Anti-Cortisol Binding Globulin antibody - N-terminal
    See all Cortisol Binding Globulin primary antibodies
  • Description

    Rabbit polyclonal to Cortisol Binding Globulin - N-terminal
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IF, ICCmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Orangutan
  • Immunogen

    Synthetic peptide corresponding to Human Cortisol Binding Globulin aa 102-120 (N terminal).
    Sequence:

    ETEIHQGFQHLHQLFAKSD


    Database link: P08185

  • Positive control

    • HeLa, A431, U87, 22RV1 and PANC cell lysates: HepG2 cells.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
  • Storage buffer

    Preservatives: 0.025% Thimerosal (merthiolate), 0.025% Sodium azide
    Constituents: 2.5% BSA, 0.45% Sodium chloride, 0.1% Dibasic monohydrogen sodium phosphate
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab191053 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB Use a concentration of 0.1 - 0.5 µg/ml. Predicted molecular weight: 45 kDa.
IHC-P Use a concentration of 0.5 - 1 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF Use a concentration of 0.5 - 1 µg/ml.
ICC Use a concentration of 0.5 - 1 µg/ml.

Target

  • Function

    Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species.
  • Tissue specificity

    Plasma; synthesized in liver. Has also been identified in a number of glycocorticoid responsive cells.
  • Involvement in disease

    Defects in SERPINA6 are a cause of corticosteroid-binding globulin deficiency (CBG deficiency) [MIM:611489]. CBG deficiency is an extremely rare hereditary disorder characterized by reduced corticosteroid-binding capacity with normal or low plasma corticosteroid-binding globulin concentration, and normal or low basal cortisol levels associated with hypo/hypertension and muscle fatigue.
  • Sequence similarities

    Belongs to the serpin family.
  • Post-translational
    modifications

    N-glycosylated; binds 5 oligosaccharide chains.
    Glycosylation in position Asn-260 is needed for steroid binding.
  • Cellular localization

    Secreted.
  • Information by UniProt
  • Database links

  • Alternative names

    • CBG antibody
    • CBG_HUMAN antibody
    • corticosteroid binding globulin antibody
    • Corticosteroid-binding globulin antibody
    • serine (or cysteine) proteinase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 6 antibody
    • Serpin A6 antibody
    • serpin peptidase inhibitor clade A (alpha 1 antiproteinase antitrypsin) member 6 antibody
    • Serpina6 antibody
    • Transcortin antibody
    see all

Images

  • All lanes : Anti-Cortisol Binding Globulin antibody - N-terminal (ab191053) at 0.5 µg

    Lane 1 : HeLa cell lysate
    Lane 2 : A431 cell lysate
    Lane 3 : U87 cell lysate
    Lane 4 : 22RV1 cell lysate
    Lane 5 : PANC cell lysate

    Predicted band size: 45 kDa

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue labelling Cortisol Binding Globulin with ab191053 at 1μg/ml. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. Biotinylated goat anti-rabbit IgG was used as secondary antibody. The tissue section was developed using Strepavidin-Biotin-Complex, with DAB as the chromogen.

  • Immunocytochemical analysis of HepG2 cells labeling Cortisol Binding Globulin with ab191053 at 1 µg/ml.

References

ab191053 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

There are currently no Customer reviews or Questions for ab191053.
Please use the links above to contact us or submit feedback about this product.

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
For licensing inquiries, please contact partnerships@abcam.com

Sign up