Overview

  • Product name
    Cortisol ELISA Kit
    See all Cortisol kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Overall 60 <=9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 30 <=9.8%
  • Sample type
    Urine, Serum, Heparin Plasma, Citrate Plasma
  • Assay type
    Competitive
  • Sensitivity
    = 2.44 ng/ml
  • Range
    10 ng/ml - 500 ng/ml
  • Recovery

    103.56 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in serum and plasma.

     

    A 96-well plate has been precoated with anti-Cortisol IgG. Samples and the Cortisol-HRP conjugate are added to the wells, where any Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

Applications

Our Abpromise guarantee covers the use of ab108665 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent dilution.

Images

  • Biological fluids for ab108665
    Biological fluids for ab108665

    Cortisol measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 1-2 fold.

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab108665

Protocols

References

This product has been referenced in:
  • Miller JL  et al. Oxytocin treatment in children with Prader-Willi syndrome: A double-blind, placebo-controlled, crossover study. Am J Med Genet A 173:1243-1250 (2017). Read more (PubMed: 28371242) »
  • Aerts J  et al. Scales tell a story on the stress history of fish. PLoS One 10:e0123411 (2015). Fish . Read more (PubMed: 25922947) »

See all 2 Publications for this product

Publishing research using ab108665? Please let us know so that we can cite the reference in this datasheet.

Customer reviews and Q&As

Submit a review Submit a question

8 reviews or Q&A

10
Votes: Highest First

Do you happen to sell Cortisol-HRP separately?

We do not sell the Cortisol-HRP reagent separately from this kit.

Test de la réactivité croisée du kit cortisol avec la corticostérone

 Average Excellent 5/5 (Ease of Use)
Abreviews
Tested the kit in mouse. I was interested in seeing if
this kit cross-reacts with corticosterone.

I have tested this kit with different plasma samples of which I knew the concentration of corticosterone.
abreview image
Username

Ms. Isabelle Barnola

Verified customer

Submitted Apr 02 2014

excellent product

 Excellent Excellent 5/5 (Ease of Use)
Abreviews
very easy to use and accurate results in a few hours
Username

Abcam user community

Verified customer

Submitted Jan 29 2014

We have bought two abcam ELISA kits to dose testosterone (ab108666) and
cortisol (ab108665) from human blood samples.
Unfortunately, blood samples were frozen without separating blood
products (no centrifugation before freezing). As a re...

Read More


I confirmed with the lab and unfortunately do not have good news:
The heavily hemolyzed samples would have an great influence on the results. We do not know of any protocol trick to circumvent this and therefore recommend to collect new sampl...

Read More

I am using this kit for mouse samples, and the amount of sample is small. can i use EDTA or something else to prevent clotting, so I can maximaze the amount of sample i get?

Thank you for contacting us.

The Cortisol ELISA kit is validated for use on Human serum and plasma samples. We have not validated the use of Mouse samples in this kit, however, plasma samples have been validated. As such, it should be ok to co...

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Avez-vous également des kits ELISA pour doser de l'ACTH plasmatique et du CRH spinal (échantillon de liquide céphalo-rachidien) chez le macaque s'il vous plait?

Malheureusement non, nous n'avons pas de kit pour le dosage de l'ACTH ou du CRH spinal.

Désolé de ne pas pouvoir vous aider davantage. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.


Je me permets de vous contacter sur les conseils d'Evelyne Doudnikoff.
Je suis à la recherche d'un kit ELISA pour doser du cortisol plasmatique chez le macaque.
Vendez-vous ce produit s'il vous plait?
Si oui, pourrais-je avoir l...

Read More

Merci de nous avoir contactés.

Nous avons à notre catalogue le kit https://www.abcam.com/ab108665, Cortisol Human (competitive)ELISA Kit.


Ce kit a été testé chez l'humain uniquement, il fonctio...

Read More

Does this Cortisol ELISA Kit recognize total or free Cortisol?

Thank you for contacting us.  The Cortisol ELISA Kit ab108665 detects total Cortisol.  I hope this helps, please let me know if you need any additional information.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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