Cortisol ELISA Kit (ab108665)
Key features and details
- Sensitivity: 2.44 ng/ml
- Range: 10 ng/ml - 500 ng/ml
- Sample type: Cit plasma, Hep Plasma, Serum, Urine
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Overview
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Product name
Cortisol ELISA Kit
See all Cortisol kits -
Detection method
Colorimetric -
Precision
Intra-assay Sample n Mean SD CV% Overall 60 <=9% Inter-assay Sample n Mean SD CV% Overall 30 <=9.8% -
Sample type
Urine, Serum, Hep Plasma, Cit plasma -
Assay type
Competitive -
Sensitivity
= 2.44 ng/ml -
Range
10 ng/ml - 500 ng/ml -
Recovery
103.56 %
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Assay duration
Multiple steps standard assay -
Species reactivity
Reacts with: Human -
Product overview
Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in serum and plasma.
A 96-well plate has been precoated with anti-Cortisol IgG. Samples and the Cortisol-HRP conjugate are added to the wells, where any Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.
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Platform
Microplate
Properties
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Storage instructions
Store at +4°C. Please refer to protocols. -
Components 1 x 96 tests 10X Washing Solution 1 x 50ml Anti-Cortisol (IgG) Coated Microplate (12 x 8 wells) 1 unit Cortisol Control 1 x 1ml Cortisol Standard 0 – 0 ng/mL 1 x 1ml Cortisol Standard 1 – 10 ng/mL 1 x 1ml Cortisol Standard 2 – 50 ng/mL 1 x 1ml Cortisol Standard 3 – 150 ng/mL 1 x 1ml Cortisol Standard 4 – 500 ng/mL 1 x 1ml Cortisol-HRP conjugate 1 x 21ml Stop Solution 1 x 15ml TMB Substrate Solution 1 x 15ml -
Research areas
Images
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Biological fluids for ab108665
Cortisol measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 1-2 fold.
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Typical Standard CurveRepresentative Standard Curve using ab108665
Datasheets and documents
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SDS download
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Datasheet download
References (10)
ab108665 has been referenced in 10 publications.
- Djordjevic B et al. Comparison of Circulating Markers and Mucosal Immune Parameters from Skin and Distal Intestine of Atlantic Salmon in Two Models of Acute Stress. Int J Mol Sci 22:N/A (2021). PubMed: 33494146
- Chang M et al. Genistein acts as antidepressant agent against chronic mild stress-induced depression model of rats through augmentation of brain-derived neurotrophic factor. Brain Behav 11:e2300 (2021). PubMed: 34333865
- Khaliq W et al. Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors. Comput Struct Biotechnol J 18:3678-3691 (2020). PubMed: 33304464
- Hong J et al. Incomplete Spinal Cord Injury Reverses the Level-Dependence of Spinal Cord Injury Immune Deficiency Syndrome. Int J Mol Sci 20:N/A (2019). PubMed: 31374824
- Ribeiro-da-Silva M et al. Interplay between sympathetic nervous system and inflammation in aseptic loosening of hip joint replacement. Sci Rep 8:16044 (2018). PubMed: 30375409
- Kotb El-Sayed MI et al. Neural and Endocrinal Pathobiochemistry of Vitiligo: Comparative Study for a Hypothesized Mechanism. Front Endocrinol (Lausanne) 9:197 (2018). PubMed: 29922226
- Frank BS et al. Movement profile influences systemic stress and biomechanical resilience to high training load exposure. J Sci Med Sport N/A:N/A (2018). PubMed: 29983354
- Miller JL et al. Oxytocin treatment in children with Prader-Willi syndrome: A double-blind, placebo-controlled, crossover study. Am J Med Genet A 173:1243-1250 (2017). PubMed: 28371242
- Aerts J et al. Scales tell a story on the stress history of fish. PLoS One 10:e0123411 (2015). Fish . PubMed: 25922947
- Norheim F et al. Regulation of angiopoietin-like protein 4 production during and after exercise. Physiol Rep 2:N/A (2014). PubMed: 25138789