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  1. Link

    cortisol-elisa-kit-ab108665.pdf

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Signal Transduction Growth Factors/Hormones Hormones
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Cortisol ELISA Kit (ab108665)

  • Datasheet
  • SDS
  • Protocol Booklet
Reviews (4)Q&A (6)References (10)

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Biological fluids for ab108665
  • Typical Standard Curve

Key features and details

  • Sensitivity: 2.44 ng/ml
  • Range: 10 ng/ml - 500 ng/ml
  • Sample type: Cit plasma, Hep Plasma, Serum, Urine
  • Detection method: Colorimetric
  • Assay type: Competitive
  • Reacts with: Human

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Overview

  • Product name

    Cortisol ELISA Kit
    See all Cortisol kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Overall 60 <=9%
    Inter-assay
    Sample n Mean SD CV%
    Overall 30 <=9.8%
  • Sample type

    Urine, Serum, Hep Plasma, Cit plasma
  • Assay type

    Competitive
  • Sensitivity

    = 2.44 ng/ml
  • Range

    10 ng/ml - 500 ng/ml
  • Recovery

    103.56 %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in serum and plasma.

     

    A 96-well plate has been precoated with anti-Cortisol IgG. Samples and the Cortisol-HRP conjugate are added to the wells, where any Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.

  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Washing Solution 1 x 50ml
    Anti-Cortisol (IgG) Coated Microplate (12 x 8 wells) 1 unit
    Cortisol Control 1 x 1ml
    Cortisol Standard 0 – 0 ng/mL 1 x 1ml
    Cortisol Standard 1 – 10 ng/mL 1 x 1ml
    Cortisol Standard 2 – 50 ng/mL 1 x 1ml
    Cortisol Standard 3 – 150 ng/mL 1 x 1ml
    Cortisol Standard 4 – 500 ng/mL 1 x 1ml
    Cortisol-HRP conjugate 1 x 21ml
    Stop Solution 1 x 15ml
    TMB Substrate Solution 1 x 15ml
  • Research areas

    • Signal Transduction
    • Growth Factors/Hormones
    • Hormones
    • Kits/ Lysates/ Other
    • Kits
    • ELISA Kits
    • ELISA Kits
    • Growth factors and hormones ELISA kits

Images

  • Biological fluids for ab108665
    Biological fluids for ab108665

    Cortisol measured in biological fluids showing quantity (ng) per mL of tested sample. Samples were diluted 1-2 fold.

  • Typical Standard Curve
    Typical Standard Curve

    Representative Standard Curve using ab108665

Protocols

  • Protocol Booklet

Click here to view the general protocols

Datasheets and documents

  • SDS download

  • Datasheet download

    Download

References (10)

Publishing research using ab108665? Please let us know so that we can cite the reference in this datasheet.

ab108665 has been referenced in 10 publications.

  • Djordjevic B  et al. Comparison of Circulating Markers and Mucosal Immune Parameters from Skin and Distal Intestine of Atlantic Salmon in Two Models of Acute Stress. Int J Mol Sci 22:N/A (2021). PubMed: 33494146
  • Chang M  et al. Genistein acts as antidepressant agent against chronic mild stress-induced depression model of rats through augmentation of brain-derived neurotrophic factor. Brain Behav 11:e2300 (2021). PubMed: 34333865
  • Khaliq W  et al. Lipid metabolic signatures deviate in sepsis survivors compared to non-survivors. Comput Struct Biotechnol J 18:3678-3691 (2020). PubMed: 33304464
  • Hong J  et al. Incomplete Spinal Cord Injury Reverses the Level-Dependence of Spinal Cord Injury Immune Deficiency Syndrome. Int J Mol Sci 20:N/A (2019). PubMed: 31374824
  • Ribeiro-da-Silva M  et al. Interplay between sympathetic nervous system and inflammation in aseptic loosening of hip joint replacement. Sci Rep 8:16044 (2018). PubMed: 30375409
  • Kotb El-Sayed MI  et al. Neural and Endocrinal Pathobiochemistry of Vitiligo: Comparative Study for a Hypothesized Mechanism. Front Endocrinol (Lausanne) 9:197 (2018). PubMed: 29922226
  • Frank BS  et al. Movement profile influences systemic stress and biomechanical resilience to high training load exposure. J Sci Med Sport N/A:N/A (2018). PubMed: 29983354
  • Miller JL  et al. Oxytocin treatment in children with Prader-Willi syndrome: A double-blind, placebo-controlled, crossover study. Am J Med Genet A 173:1243-1250 (2017). PubMed: 28371242
  • Aerts J  et al. Scales tell a story on the stress history of fish. PLoS One 10:e0123411 (2015). Fish . PubMed: 25922947
  • Norheim F  et al. Regulation of angiopoietin-like protein 4 production during and after exercise. Physiol Rep 2:N/A (2014). PubMed: 25138789

Customer reviews and Q&As

Show All Reviews Q&A
Submit a review Submit a question

1-10 of 10 Abreviews or Q&A

Impact of physical activity and fitness level on physiological and psychological stress in individuals with spinal cord injury.

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Very impressed with this kit. I used this kit to measure total Cortisol in humans. The sample was originally taken with EDTA serum and worked well.
Very straight forward protocol, all the standards pre-mixed and clear results. Will definitely order again!
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Mr. Gabriel Dix

Verified customer

Submitted Dec 23 2020

Hair Cortisol as a Biomarker of Chronic Stress among Saudi Women who are Survivors of Wife Abuse

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
I have used this kit to analyze human cortisol concentration in hair sample. It is very easy to use.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jun 12 2019

Test de la réactivité croisée du kit cortisol avec la corticostérone

Average Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
abreview image
Tested the kit in mouse. I was interested in seeing if
this kit cross-reacts with corticosterone.

I have tested this kit with different plasma samples of which I knew the concentration of corticosterone.
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Ms. Isabelle Barnola

Verified customer

Submitted Apr 02 2014

excellent product

Excellent Excellent 5/5 (Ease of Use)
Abreviews
Abreviews
very easy to use and accurate results in a few hours
The reviewer received a reward from Abcam’s Loyalty Program in thanks for submitting this Abreview and for helping the scientific community make better-informed decisions.

Abcam user community

Verified customer

Submitted Jan 29 2014

Question

Do you happen to sell Cortisol-HRP separately?

Read More

Abcam community

Verified customer

Asked on Jun 06 2014

Answer

We do not sell the Cortisol-HRP reagent separately from this kit.

Read More

Jeremy Kasanov

Abcam Scientific Support

Answered on Jun 06 2014

Question

We have bought two abcam ELISA kits to dose testosterone (ab108666) and
cortisol (ab108665) from human blood samples.
Unfortunately, blood samples were frozen without separating blood
products (no centrifugation before freezing). As a result, there has
been an heavy hemolysis in our samples.
Since these samples cannot be taken again, I have two questions:
- is it still possible to get at least a &apos;relative&apos; measure of cortisol
and/or testosterone or is it absolutely impossible?
- is there some specific recommendations for our situation? Diluting
samples? Centrifugating more to remove big cell components?

Read More

Abcam community

Verified customer

Asked on Apr 24 2013

Answer


I confirmed with the lab and unfortunately do not have good news:
The heavily hemolyzed samples would have an great influence on the results. We do not know of any protocol trick to circumvent this and therefore recommend to collect new samples.
But we would assume that all samples would be interfere with the assay at the same level.

Read More

Abcam Scientific Support

Answered on Apr 24 2013

Question

I am using this kit for mouse samples, and the amount of sample is small. can i use EDTA or something else to prevent clotting, so I can maximaze the amount of sample i get?

Read More

Abcam community

Verified customer

Asked on Mar 05 2013

Answer

Thank you for contacting us.

The Cortisol ELISA kit is validated for use on Human serum and plasma samples. We have not validated the use of Mouse samples in this kit, however, plasma samples have been validated. As such, it should be ok to collect the mouse samples with EDTA to prevent clotting. The sample should be centrifuged and the plasma collected and tested or frozen until tested.


I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Read More

Abcam Scientific Support

Answered on Mar 05 2013

Question

Avez-vous également des kits ELISA pour doser de l'ACTH plasmatique et du CRH spinal (échantillon de liquide céphalo-rachidien) chez le macaque s'il vous plait?

Read More

Abcam community

Verified customer

Asked on Jun 07 2012

Answer

Malheureusement non, nous n'avons pas de kit pour le dosage de l'ACTH ou du CRH spinal.

Désolé de ne pas pouvoir vous aider davantage. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Read More

Abcam Scientific Support

Answered on Jun 07 2012

Question


Je me permets de vous contacter sur les conseils d'Evelyne Doudnikoff.
Je suis à la recherche d'un kit ELISA pour doser du cortisol plasmatique chez le macaque.
Vendez-vous ce produit s'il vous plait?
Si oui, pourrais-je avoir le prix et le mode d'emploi de ce kit svp?

Read More

Abcam community

Verified customer

Asked on Jun 07 2012

Answer

Merci de nous avoir contactés.

Nous avons à notre catalogue le kit https://www.abcam.com/ab108665, Cortisol Human (competitive)ELISA Kit.


Ce kit a été testé chez l'humain uniquement, il fonctionne pour le serum et plasma.

J'espère que ces informations vous seront utiles. N'hésitez pas à nous contacter de nouveau si vous avez d'autres questions.

Read More

Abcam Scientific Support

Answered on Jun 07 2012

Question

Does this Cortisol ELISA Kit recognize total or free Cortisol?

Read More

Abcam community

Verified customer

Asked on Nov 09 2011

Answer

Thank you for contacting us.  The Cortisol ELISA Kit ab108665 detects total Cortisol.  I hope this helps, please let me know if you need any additional information. 

Read More

Abcam Scientific Support

Answered on Nov 09 2011

Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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