Overview

  • Product name

    Cortisol ELISA Kit
    See all Cortisol kits
  • Detection method

    Colorimetric
  • Precision

    Intra-assay
    Sample n Mean SD CV%
    Urine 60 <=6.5%
    Inter-assay
    Sample n Mean SD CV%
    Urine 30 <=7.2%
  • Sample type

    Urine
  • Assay type

    Competitive
  • Sensitivity

    2.95 ng/ml
  • Range

    10 ng/ml - 500 ng/ml
  • Recovery

    107.48 %

  • Assay duration

    Multiple steps standard assay
  • Species reactivity

    Reacts with: Human
  • Product overview

    Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in urine.


    A 96-well plate has been precoated with anti-Cortisol. Samples and the Cortisol-HRP conjugate are added to the wells, where Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.

  • Tested applications

    Suitable for: Competitive ELISAmore details
  • Platform

    Microplate

Properties

  • Storage instructions

    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Solution 1 x 50ml
    Anti-Cortisol IgG Coated Microplate (12 x 8 wells) 1 unit
    Cortisol Standard 0 - 0 ng/mL 1 x 4ml
    Cortisol Standard 1 - 1 ng/mL 1 x 1ml
    Cortisol Standard 2 - 5 ng/mL 1 x 1ml
    Cortisol Standard 3 - 30 ng/mL 1 x 1ml
    Cortisol Standard 4 – 200 ng/mL 1 x 1ml
    Cortisol-HRP Conjugate 1 x 33ml
    Cover foils 1 unit
    High control 1 x 1ml
    Low control 1 x 1ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab108674 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab108674

Protocols

References

ab108674 has not yet been referenced specifically in any publications.

Customer reviews and Q&As

1-4 of 4 Abreviews or Q&A

Answer

Thank you for contacting us.

This product comes with full money back guarantee if used with human urine samples. Please reassure them if the product does not work they will get refund or replacements.

I would advise them buying 1 product first for testing, if it works then they can go ahead and buy the rest.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Free Rabbit monoclonal antibody with any purchase of a primary antibody, while stocks last! Quote “RABMAB-XBSMG” in your next primary antibody order. For more information, visit the following link: https://www.abcam.com/index.html?pageconfig=resource&rid=15447

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Answer

Thank you for contacting us.

We do have several kits available against the products requested. However, none of them have unfortunately been tested with fish.

What I would suggest is that you have a look at the products identified, and let me know which are the ones you would be interested in. I could then try to find out the likelihood of them to work in that species.

Against Cortisol: ab108665 and ab108674.

Against Glucose: ab136956, ab136955, ab102517 and ab65333.

Against Lactate: ab65330 and ab65331.

I look forward to receiving your reply. Have a nice day.



No dudes en contactarme en castellano si lo prefieres.

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Question

As I’m not familiar with these items, I tried to find the ab codes through your site.
For the items I found a relative code I have update the list.
For those I don’t have a reference code, this is because either no products matches or all available products reacts for humans when we need them for pigs.
So please check the list again and advice.
https://www.abcam.com/Procalcitonin-Human-ELISA-Kit-ab100630.html Porcine Procalcitonin, PCT ELISA kit 96 Tests
https://www.abcam.com/IL6-Pig-ELISA-Kit-1-x-96-Well-Plate-ab100755.html Porcine IL-6 Quantikine ELISA kit 96 Tests
https://www.abcam.com/TNF-alpha-Porcine-ELISA-Kit-ab100756.html Porcine TNF-alpha/ TNFSF1A Quantikine ELISA kit 96 Tests
https://www.abcam.com/Cortisol-Human-ELISA-Kit-ab108674.html Porcine Cortisol ELISA kit 96 Tests ? (Is it the same for humans and pigs?)
Porcine Noradrenalin ELISA kit 96 Tests (No product match)
Porcine Adrenaline ELISA kit 96 Tests (No product match)
https://www.abcam.com/Albumin-Swine-ELISA-Kit-ab108794.html Porcine Serum Cystatin C ELISA Kit 96 Tests
Porcine Urinary Cystatin C ELISA Kit 96 Tests (Only for humans)
https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Serum NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests
Porcine Urinary NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests (Only for humans)
Porcine Urinary KIM-1 (Kidney Injury Molecule -1) ELISA Kit 96 Tests (Only for humans)
https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Urinary IL-18 (Interleukin -18) ELISA Kit 96 Tests
Porcine Urinary NAG (N-Acetyl-β-D-Glucosaminidase) ELISA Kit96 Tests (Only for humans)
https://www.abcam.com/IFN-gamma-Pig-ELISA-Kit-ab113353.html Porcine Urinary L-FABP (Liver-type Fatty-Acid Binding Protein) ELISA Kit 96 Tests

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Answer

I have received information regarding

ab119589 Cystatin C Human ELISA Kit
ab119590 Cystatin C Mouse ELISA Kit
ab119596 KIM1 Mouse ELISA Kit
ab119597 KIM1 Rat ELISA Kit
ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit
ab119601 Lipocalin-2 / NGAL Mouse ELISA Kit

these has not been tested with Swine or pig samples so we are unable to confirm the species cross reactivity. It mean only ab100755, ab100756, ab108674, ab108794, ab113352, ab113353 are OK to use with Porcine samples and are guaranteed. Would you like to have a quote for these antibodies.

I hope this info will be helpful. Should you require more information please do not hesitate to contact me.

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Question

As I’m not familiar with these items, I tried to find the ab codes through your site.
For the items I found a relative code I have update the list.
For those I don’t have a reference code, this is because either no products matches or all available products reacts for humans when we need them for pigs.
So please check the list again and advice.
https://www.abcam.com/Procalcitonin-Human-ELISA-Kit-ab100630.html Porcine Procalcitonin, PCT ELISA kit 96 Tests
https://www.abcam.com/IL6-Pig-ELISA-Kit-1-x-96-Well-Plate-ab100755.html Porcine IL-6 Quantikine ELISA kit 96 Tests
https://www.abcam.com/TNF-alpha-Porcine-ELISA-Kit-ab100756.html Porcine TNF-alpha/ TNFSF1A Quantikine ELISA kit 96 Tests
https://www.abcam.com/Cortisol-Human-ELISA-Kit-ab108674.html Porcine Cortisol ELISA kit 96 Tests ? (Is it the same for humans and pigs?)
Porcine Noradrenalin ELISA kit 96 Tests (No product match)
Porcine Adrenaline ELISA kit 96 Tests (No product match)
https://www.abcam.com/Albumin-Swine-ELISA-Kit-ab108794.html Porcine Serum Cystatin C ELISA Kit 96 Tests
Porcine Urinary Cystatin C ELISA Kit 96 Tests (Only for humans)
https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Serum NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests
Porcine Urinary NGAL (Neutrophil Gelatinase-Associated Lipocalin) ELISA Kit 96 Tests (Only for humans)
Porcine Urinary KIM-1 (Kidney Injury Molecule -1) ELISA Kit 96 Tests (Only for humans)
https://www.abcam.com/IL8-Pig-ELISA-Kit-ab113352.html Porcine Urinary IL-18 (Interleukin -18) ELISA Kit 96 Tests
Porcine Urinary NAG (N-Acetyl-β-D-Glucosaminidase) ELISA Kit96 Tests (Only for humans)
https://www.abcam.com/IFN-gamma-Pig-ELISA-Kit-ab113353.html Porcine Urinary L-FABP (Liver-type Fatty-Acid Binding Protein) ELISA Kit 96 Tests

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Answer

Thank you for contacting us.


To let you know, we are currently investigating the Porcine (pig) species, cross-reactivity information for following kits ab119602, ab113326, ab119600, ab119601, ab119590 and ab119589. We will send you the details soon.

The other kits ab100755, ab100756, ab108674, ab108794, ab113352, ab113353 are OK to use with Porcine samples and are guaranteed.

I hope this information is helpful to you. Please do not hesitate to contact us if you need any more advice or information.

Use our products? Submit an Abreview. Earn rewards!
https://www.abcam.com/abreviews

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