Overview

  • Product name
    Cortisol ELISA Kit
    See all Cortisol kits
  • Detection method
    Colorimetric
  • Precision
    Intra-assay
    Sample n Mean SD CV%
    Saliva 60 <=10%
    Inter-assay
    Sample n Mean SD CV%
    Saliva 30 <=8.3%
  • Sample type
    Saliva
  • Assay type
    Competitive
  • Sensitivity
    0.12 ng/ml
  • Range
    0.5 ng/ml - 100 ng/ml
  • Recovery

    95.42 %

  • Assay duration
    Multiple steps standard assay
  • Species reactivity
    Reacts with: Human
  • Product overview

    Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in saliva.


    A 96-well plate has been precoated with anti-Cortisol IgG. Samples and the Cortisol-HRP conjugate are added to the wells, where Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.

  • Tested applications
    Suitable for: Competitive ELISAmore details
  • Platform
    Microplate

Properties

  • Storage instructions
    Store at +4°C. Please refer to protocols.
  • Components 1 x 96 tests
    10X Wash Solution 1 x 50ml
    Anti-Cortisol IgG Coated Microplate (12 x 8 wells) 1 unit
    Control A 1 x 1ml
    Control B 1 x 1ml
    Cortisol Standard 0 – 0 ng/mL 1 x 1ml
    Cortisol Standard 1 - 0.5 ng/mL 1 x 1ml
    Cortisol Standard 2 – 1 ng/mL 1 x 1ml
    Cortisol Standard 3 – 5 ng/mL 1 x 1ml
    Cortisol Standard 4 – 10 ng/mL 1 x 1ml
    Cortisol Standard 5 – 20 ng/mL 1 x 1ml
    Cortisol Standard 6 – 100 ng/mL 1 x 1ml
    Cortisol-HRP Conjugate (Concentrated) 1 x 1ml
    Foil Cover 1 unit
    Incubation Buffer 1 x 30ml
    Stop Solution 1 x 15ml
    Strip holder 1 unit
    TMB Substrate Solution 1 x 15ml
  • Research areas

Associated products

Applications

Our Abpromise guarantee covers the use of ab154996 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
Competitive ELISA Use at an assay dependent concentration.

Images

  • Representative Standard Curve using ab154996.

Protocols

References

This product has been referenced in:
  • Ruiz-Babot G  et al. Modeling Congenital Adrenal Hyperplasia and Testing Interventions for Adrenal Insufficiency Using Donor-Specific Reprogrammed Cells. Cell Rep 22:1236-1249 (2018). Read more (PubMed: 29386111) »
See 1 Publication for this product

Customer reviews and Q&As

Answer



For ab154996 we recommend to collect saliva samples with a centrifuge glass tube and a plastic straw or with the “Salivette” (Sarstedt, Ref. 511534500).

The following should be noted:
a) If saliva collection is carried out in the morning ensure that this is carried out prior to brushing teeth
b) During the day allow 1 hour after any food or drink before collecting saliva samples
c) It is very important that a good clear sample is received – i.e. no contamination with food, lipstick, blood
(bleeding gums) or other extraneous materials.


Processing Instructions
1. Let the saliva flow down through the straw into the centrifuge glass tube
2. Centrifuge at 3000 rpm for 15 min.
3. Store at -20°C for at least 1 hour
4. Defrost samples
5. Centrifuge again for 15 min. at 3000 rpm
6. The saliva sample is now ready to be tested.
7. Store the sample at 4 °C for one week or at -20°C for longer term storage.


Saliva Processing Instructions with Salivette Sarstedt
1. Remove the swab from the suspended insert of the Salivette.
2. Gently chewing the swab for 1 minute produces a sufficient quantity of Saliva.
3. Replace the swab into the Salivette and firmly close the tube using the stopper.
4. Centrifuge the Salivette for 2 minutes at 1000g (rcf) for saliva generation.
5. Remove the insert complete with the swab from the centrifuge vessel and discard. The clear Saliva is now
ready for analysis (at least 1 ml of saliva should be recovered with this method).

For storage of samples we would recommend to freeze them at -20C. We have not tested how long samples can be stored frozen without loosing quality but believe a couple month should not be a problem.

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Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

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