Key features and details
- Sensitivity: 0.12 ng/ml
- Range: 0.5 ng/ml - 100 ng/ml
- Sample type: Saliva
- Detection method: Colorimetric
- Assay type: Competitive
- Reacts with: Human
Product nameCortisol ELISA Kit
See all Cortisol kits
Intra-assay Sample n Mean SD CV% Saliva 60 <=10% Inter-assay Sample n Mean SD CV% Saliva 30 <=8.3%
Range0.5 ng/ml - 100 ng/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
Abcam’s Cortisol in vitro competitive ELISA (Enzyme-Linked Immunosorbent Assay) kit is designed for the accurate quantitative measurement of Cortisol in saliva.
A 96-well plate has been precoated with anti-Cortisol IgG. Samples and the Cortisol-HRP conjugate are added to the wells, where Cortisol in the sample competes with the added Cortisol-HRP for antibody binding. After incubation, the wells are washed to remove unbound material and TMB substrate is then added which is catalyzed by HRP to produce blue coloration. The reaction is terminated by addition of Stop Solution which stops the color development and produces a color change from blue to yellow. The intensity of signal is inversely proportional to the amount of Cortisol in the sample and the intensity is measured at 450 nm.
Storage instructionsStore at +4°C. Please refer to protocols.
Components 1 x 96 tests 10X Wash Solution 1 x 50ml Anti-Cortisol IgG Coated Microplate (12 x 8 wells) 1 unit Control A 1 x 1ml Control B 1 x 1ml Cortisol Standard 0 – 0 ng/mL 1 x 1ml Cortisol Standard 1 - 0.5 ng/mL 1 x 1ml Cortisol Standard 2 – 1 ng/mL 1 x 1ml Cortisol Standard 3 – 5 ng/mL 1 x 1ml Cortisol Standard 4 – 10 ng/mL 1 x 1ml Cortisol Standard 5 – 20 ng/mL 1 x 1ml Cortisol Standard 6 – 100 ng/mL 1 x 1ml Cortisol-HRP Conjugate (Concentrated) 1 x 1ml Foil Cover 1 unit Incubation Buffer 1 x 30ml Stop Solution 1 x 15ml Strip holder 1 unit TMB Substrate Solution 1 x 15ml
ab154996 has been referenced in 1 publication.
- Ruiz-Babot G et al. Modeling Congenital Adrenal Hyperplasia and Testing Interventions for Adrenal Insufficiency Using Donor-Specific Reprogrammed Cells. Cell Rep 22:1236-1249 (2018). PubMed: 29386111