Key features and details
- Rabbit polyclonal to COSMC
- Suitable for: IHC-P, WB, ICC/IF
- Reacts with: Mouse, Human
- Isotype: IgG
Product nameAnti-COSMC antibody
See all COSMC primary antibodies
DescriptionRabbit polyclonal to COSMC
Tested applicationsSuitable for: IHC-P, WB, ICC/IFmore details
Species reactivityReacts with: Mouse, Human
Predicted to work with: Rat, Cow
- WB: Mouse gonadal and liver lysates. IHC-P: Human kidney and gastric cancer tissues. ICC/IF: HeLa cells.
This product was previously labelled as C1GALT1C1
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We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
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Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C long term. Avoid freeze / thaw cycle.
Storage bufferpH: 7.30
Preservative: 0.02% Sodium azide
Constituents: PBS, 50% Glycerol (glycerin, glycerine)
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab229831 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/20 - 1/200.|
|WB||1/500 - 1/2000. Detects a band of approximately 36 kDa (predicted molecular weight: 36 kDa).|
|ICC/IF||1/50 - 1/200.|
FunctionProbable chaperone required for the generation of 1 O-glycan Gal-beta1-3GalNAc-alpha1-Ser/Thr (T antigen), which is a precursor for many extended O-glycans in glycoproteins. Probably acts as a specific molecular chaperone assisting the folding/stability of core 1 beta-3-galactosyltransferase (C1GALT1).
Tissue specificityUbiquitously expressed. Abundantly expressed in salivary gland, stomach, small intestine, kidney, and testis and at intermediate levels in whole brain, cerebellum, spinal cord, thymus, spleen, trachea, lung, pancreas, ovary, and uterus.
Involvement in diseaseDefects in C1GALT1C1 are the cause of Tn syndrome (TNSYN) [MIM:300622]. Tn syndrome is a rare autoimmune disease caused by somatic mutation in the C1GALT1C1 gene in which subpopulations of blood cells of all lineages carry an incompletely glycosylated membrane glycoprotein, i.e. the Tn antigen. Since leukocytes and platelets are affected as well as red cells, anemia, leukopenia and thrombocytopenia are features. Tn-polyagglutinability is sometimes associated with leukemia or is a preleukemic state.
Sequence similaritiesBelongs to the glycosyltransferase 31 family. Beta3-Gal-T subfamily.
- Information by UniProt
- HSPC067 antibody
- 3-galactosyltransferase 2 antibody
- Beta 1,3 galactosyltransferase 2 antibody
All lanes : Anti-COSMC antibody (ab229831) at 1/500 dilution
Lane 1 : Mouse gonadal lysate
Lane 2 : Mouse liver lysate
All lanes : Goat polyclonal to Rabbit IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa
Paraffin-embedded human kidney tissue stained for COSMC using ab229831 at 1/100 dilution in immunohistochemical analysis.
Paraffin-embedded human gastric cancer tissue stained for COSMC using ab229831 at 1/100 dilution in immunohistochemical analysis.
HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for COSMC (green) using ab229831 at 1/100 dilution in ICC/IF, followed by Alexa Fluor 488® conjugated Goat Anti-Rabbit IgG (H+L).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab229831 has not yet been referenced specifically in any publications.