Overview

  • Product name

  • Description

    Rabbit polyclonal to COUP TF1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, ICC/IF, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat, Cow
  • Immunogen

    Synthetic peptide within Human COUP TF1 aa 1-44. The exact sequence is proprietary.
    Database link: P10589

  • Positive control

    • IHC-P: Human liver and skin tissues. ICC/IF: HeLa cells. WB: COUP TF1 transfected HEK-293T cell lysate.

Properties

Applications

Our Abpromise guarantee covers the use of ab230444 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 46 kDa.
ICC/IF 1/100 - 1/1000.
IHC-P Use a concentration of 5 µg/ml. Perform heat mediated antigen retrieval before commencing with IHC staining protocol.

Suggested antigen retrieval using heat mediated 10 mM Citrate buffer (pH 6.0) or Tris-EDTA buffer (pH 8.0).

Target

  • Function

    Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG.
  • Sequence similarities

    Belongs to the nuclear hormone receptor family. NR2 subfamily.
    Contains 1 nuclear receptor DNA-binding domain.
  • Cellular localization

    Nucleus.
  • Information by UniProt
  • Database links

  • Alternative names

    • Chicken ovalbumin upstream promoter 1 antibody
    • COT1_HUMAN antibody
    • COUP transcription factor 1 antibody
    • COUP transcription factor I antibody
    • COUP-TF I antibody
    • COUP-TF1 antibody
    • EAR-3 antibody
    • EAR3 antibody
    • ERBAL3 antibody
    • NR2F1 antibody
    • NR2F2 antibody
    • Nuclear receptor subfamily 2 group F member 1 antibody
    • SVP44 antibody
    • TCFCOUP1 antibody
    • TFCOUP1 antibody
    • Transcription factor COUP 1 antibody
    • V ERBA related protein EAR 3 antibody
    • V-erbA-related protein 3 antibody
    see all

Images

  • Formalin-fixed, paraffin-embedded human skin tissue stained for COUP TF1 using ab230444 at 5 µg/ml in immunohistochemical analysis.

  • Paraformaldehyde-fixed HeLa (human epithelial cell line from cervix adenocarcinoma) cells stained for COUP TF1 (green) using ab230444 at 1/200 dilution in ICC/IF. Lower panel: Merged with DNA probe.

  • All lanes : Anti-COUP TF1 antibody (ab230444) at 1/500 dilution

    Lane 1 : Non-transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate
    Lane 2 : COUP TF1 transfected HEK-293T (human epithelial cell line from embryonic kidney transformed with large T antigen) cell lysate

    Predicted band size: 46 kDa



    12% SDS-PAGE gel.

  • Formalin-fixed, paraffin-embedded human liver tissue stained for COUP TF1 using ab230444 at 5 µg/ml in immunohistochemical analysis.

References

ab230444 has not yet been referenced specifically in any publications.

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