Recombinant Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free (ab224272)
Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR10841] to COUP TF1 - BSA and Azide free
- Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
Related conjugates and formulations
Overview
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Product name
Anti-COUP TF1 antibody [EPR10841] - BSA and Azide free
See all COUP TF1 primary antibodies -
Description
Rabbit monoclonal [EPR10841] to COUP TF1 - BSA and Azide free -
Host species
Rabbit -
Specificity
The mouse and rat recommendation is based on the WB results. We do not guarantee IHC-P for mouse and rat.
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Tested applications
Suitable for: Flow Cyt (Intra), WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human -
Immunogen
Synthetic peptide. This information is proprietary to Abcam and/or its suppliers.
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Positive control
- Human fetal brain, Human fetal kidney and 293 cell lysates; Human glioblastoma tissue, Human transitional cell carcinoma tissue, and 293 cells.
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General notes
ab224272 is the carrier-free version of ab181137.
Our carrier-free antibodies are typically supplied in a PBS-only formulation, purified and free of BSA, sodium azide and glycerol. The carrier-free buffer and high concentration allow for increased conjugation efficiency.
This conjugation-ready format is designed for use with fluorochromes, metal isotopes, oligonucleotides, and enzymes, which makes them ideal for antibody labelling, functional and cell-based assays, flow-based assays (e.g. mass cytometry) and Multiplex Imaging applications.
Use our conjugation kits for antibody conjugates that are ready-to-use in as little as 20 minutes with <1 minute hands-on-time and 100% antibody recovery: available for fluorescent dyes, HRP, biotin and gold.
This product is compatible with the Maxpar® Antibody Labeling Kit from Fluidigm, without the need for antibody preparation. Maxpar® is a trademark of Fluidigm Canada Inc.
This product is a recombinant monoclonal antibody, which offers several advantages including:
- - High batch-to-batch consistency and reproducibility
- - Improved sensitivity and specificity
- - Long-term security of supply
- - Animal-free production
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Store at +4°C. Do Not Freeze. -
Storage buffer
pH: 7.2
Constituent: PBS -
Carrier free
Yes -
Concentration information loading...
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Purity
Protein A purified -
Clonality
Monoclonal -
Clone number
EPR10841 -
Isotype
IgG -
Research areas
Associated products
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Alternative Versions
- Anti-COUP TF1 antibody [EPR10841] (ab181137)
- Alexa Fluor® 488 Anti-COUP TF1 antibody [EPR10841] (ab209462)
- Alexa Fluor® 647 Anti-COUP TF1 antibody [EPR10841] (ab210038)
- Alexa Fluor® 555 Anti-COUP TF1 antibody [EPR10841] (ab211712)
- PE Anti-COUP TF1 antibody [EPR10841] (ab303116)
- APC Anti-COUP TF1 antibody [EPR10841] (ab303117)
- HRP Anti-COUP TF1 antibody [EPR10841] (ab303118)
- Alexa Fluor® 594 Anti-COUP TF1 antibody [EPR10841] (ab310445)
- Alexa Fluor® 568 Anti-COUP TF1 antibody [EPR10841] (ab312446)
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Conjugation kits
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Isotype control
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Recombinant Protein
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Related Products
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab224272 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Application | Abreviews | Notes |
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Flow Cyt (Intra) |
Use at an assay dependent concentration.
ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
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WB |
Use at an assay dependent concentration. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa).
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IHC-P |
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
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ICC/IF |
Use at an assay dependent concentration.
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Notes |
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Flow Cyt (Intra)
Use at an assay dependent concentration. ab199376 - Rabbit monoclonal IgG, is suitable for use as an isotype control with this antibody. |
WB
Use at an assay dependent concentration. Detects a band of approximately 46 kDa (predicted molecular weight: 46 kDa). |
IHC-P
Use at an assay dependent concentration. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
ICC/IF
Use at an assay dependent concentration. |
Target
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Function
Coup (chicken ovalbumin upstream promoter) transcription factor binds to the ovalbumin promoter and, in conjunction with another protein (S300-II) stimulates initiation of transcription. Binds to both direct repeats and palindromes of the 5'-AGGTCA-3' motif. Represses transcriptional activity of LHCG. -
Sequence similarities
Belongs to the nuclear hormone receptor family. NR2 subfamily.
Contains 1 nuclear receptor DNA-binding domain. -
Cellular localization
Nucleus. - Information by UniProt
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Database links
- Entrez Gene: 7025 Human
- Entrez Gene: 13865 Mouse
- Entrez Gene: 81808 Rat
- Omim: 132890 Human
- SwissProt: P10589 Human
- SwissProt: Q60632 Mouse
- Unigene: 519445 Human
- Unigene: 439653 Mouse
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Alternative names
- Chicken ovalbumin upstream promoter 1 antibody
- COT1_HUMAN antibody
- COUP transcription factor 1 antibody
see all
Images
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Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) analysis of human breast carcinoma tissue sections labeling COUP TF1 with Purified ab181137 at 1:100 dilution. Heat mediated antigen retrieval was performed using citrate buffer, pH6.0 . Tissue was counterstained with Hematoxylin. ImmunoHistoProbe one step HRP Polymer (ready to use) secondary antibody was used at 1:0 dilution. PBS instead of the primary antibody was used as the negative control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181137).
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Intracellular Flow Cytometry analysis of MCF7 (Human cervix adenocarcinoma epithelial cell) cells labeling COUP TF1 with purified ab181137 at 1/500 dilution (red). Cells were fixed with 4% Paraformaldehyde. A Goat anti rabbit IgG (Alexa Fluor® 488) secondary antibody was used at 1/2000 dilution. Isotype control - Rabbit monoclonal IgG (Black). Unlabeled control - Cell without incubation with primary antibody and secondary antibody (Blue). This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181137).
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Immunocytochemistry/ Immunofluorescence analysis of Jurkat (Human T cell leukemia T lymphocyte) cells labeling Coup TF2 with Purified ab181137 at 1:100 dilution. Cells were fixed in 4% Paraformaldehyde and permeabilized with 0.1% tritonX-100. Cells were counterstained with Ab195889 Anti-alpha Tubulin antibody [DM1A] - Microtubule Marker (Alexa Fluor ® 594) 1:200 (2.5 μg/ml). ab150077 Goat anti rabbit IgG(Alexa Fluor ® 488) was used as the secondary antibody at 1:1000 dilution. DAPI nuclear counterstain. PBS instead of the primary antibody was used as the secondary antibody only control.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181137).
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Immunocytochemistry/Immunofluorescence analysis of MCF-7cells labelling COUP TF1 with unpurified ab181137 at 1/500. Cells were fixed with 4% Paraformaldehyde. ab150077, an Alexa Fluor® 488-conjugated goat anti-rabbit IgG (1/1000) was used as the secondary antibody.
Control: PBS only.
Nuclear counter stain: DAPI.This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181137).
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Immunohistochemical analysis of paraffin-embedded Human glioblastoma staining COUP TF1 using unpurified ab181137 at 1/100. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody, and Hematoxylin for counterstaining.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181137).
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Intracellular flow cytometric analysis of COUP TF1 labeling 2% paraformaldehyde fixed 293 cells using unpurified ab181137 at 1/70 dilution (red), compared to a Rabbit monoclonal IgG isotype control (green). Goat anti rabbit IgG (FITC) secondary antibody was used at 1/150 dilution.
This data was developed using the same antibody clone in a different buffer formulation containing PBS, BSA, glycerol, and sodium azide (ab181137).
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This IHC data was generated using the same anti-COUP TF1 antibody clone [EPR10841] in a different buffer formulation (cat# ab181137).
Immunohistochemical analysis of paraffin-embedded Human transitional cell carcinoma staining COUP TF1 using ab181137 at 1/100. Prediluted HRP Polymer for Rabbit IgG was used as a secondary antibody, and Hematoxylin for counterstaining.
Protocols
Datasheets and documents
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Datasheet download
Certificate of Compliance
References (0)
ab224272 has not yet been referenced specifically in any publications.