Product nameCovid-19 GeoMx-formatted Antibody Panel
Covid-19 GeoMx-formatted Antibody Panel ab273594 is designed for use with NanoString Digital Spatial Profiling (DSP) technology. To use this panel you must also purchase Covid-19 GeoMx-formatted Panel Probe R Mix ab273595 and also a Core GeoMx antibody panel from Nanostring. Please also contact us (NanostringRequests@abcam.com) to request the bioinformatics file required to use this panel in your GeoMx Assay.
This antibody panel is supplied as one pre-mixed vial containing 112 µl of antibody mix sufficient for 12 slides:
- Recombinant Anti-ACE2 antibody HLA-DPB1 antibody [EPR4436] conjugated to GeoMx tag ID0314
- Recombinant Anti-TMPRSS2 antibody [EPR3861] conjugated to GeoMx tag ID0315
- Recombinant Anti-Cathepsin L/V/K/H antibody [EPR8011] conjugated to GeoMx tag ID0321
- Recombinant Anti-DDX5 antibody [EPR7239] conjugated to GeoMx tag ID0322
- Anti-SARS-cov2 Spike antibody ab272504 conjugated to GeoMx tag ID0318
NB ab272504 is a polyclonal antibody
Use this panel as a GeoMx Custom Module as explained in the GeoMx DSP Slide Preparation User Manual from NanoString (www.nanostring.com/geomx-online-user-manual). You can use this module as you would other GeoMx Modules and do not need to follow the instructions specific to custom antibody spike-ins.
Up to 5 GeoMx-formatted antibodies from Abcam can be combined into one panel for use in a GeoMx DSP Assay. This panel contains 5 antibodies so it cannot be combined with other GeoMx-formatted antibodies from Abcam. Please contact us if you would like to purchase the antibodies contained within this panel as individual GeoMx-formatted antibodies.
This panel can be combined with GeoMx Assay panels purchased from Nanostring. For a full list of pre-validated NanoString GeoMx Assays, visit www.nanostring.com/products/geomx-digital-spatial-profiler/geomx-assays
To learn more about Abcam’s GeoMx-formatted antibodies and custom conjugation services, please visit here.
To learn more about NanoString's Covid19 products, please visit www.nanostring.com/COVID19
NanoString, nCounter and GeoMx are trademarks or registered trademarks of NanoString Technologies, Inc., in the United States and/or other countries.
GeoMx-formatted TMPRSS2 antibody clone EPR3861, ACE2 antibody clone EPR4436, Cathepsin L/V/K/H antibody clone EPR8011, and DDX5 antibody clone EPR7239 tested in conventional IHC in human formalin-fixed paraffin embedded tissue sections to confirm that antibody specificity is retained after oligo-conjugation.
GeoMx-formatted SARS-COV-2 spike glycoprotein antibody tested in SARS-COV-2 spike glycoprotein overexpressing HEK293 cell line slides to confirm that antibody specificity is retained after oligo-conjugation.
The same staining pattern was seen in the conjugated antibody as with the unconjugated control antibody (unconjugated antibody data not shown).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab273594 has not yet been referenced specifically in any publications.