Key features and details
- Sample type: Plasma, Serum
- Detection method: Colorimetric
- Assay type: Semi-quantitative
Product nameCOVID-19 N-Protein Human IgA ELISA Kit
See all COVID-19 N-Protein kits
Sample typeSerum, Plasma
Assay durationMultiple steps standard assay
COVID-19 N-Protein Human IgA ELISA Kit (ab276183) is an in vitro indirect ELISA for the semi-quantitative measurement of human IgA antibody against SARS-CoV-2 N protein in human serum.
Standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARSCoV-2 N protein, which combines with the corresponding antibody present in a sample. When a secondary anti-human Antibody-HRP is added, a complex of Antibody-HRP human IgA antibody virus N antigen forms on the microplate. A TMB substrate is added and a blue color is generated. The depth of color is relative to the amount of the anti-SARS-CoV-2 IgA antibody present. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The Positive Control is from an inactivated serum sample which contains SARS-COV-2 N protein human IgA antibody. We do not know the exact amount of SARS-COV-2 N protein human IgA antibody in the Positive Control sample. The Positive Control can be used as calibration curve for interpretation purposes in different assays.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -80°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 1 x 25ml 5X Assay Diluent B 1 x 15ml HRP-anti IgA antibody 1 vial Negative Control 1 vial Positive Control 1 vial SARS-CoV-2 N protein coated Microplate 1 unit Stop Solution 1 x 8ml TMB One-Step Substrate Reagent 1 x 12ml
ab276183 has not yet been referenced specifically in any publications.