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    covid-19-n-protein-human-igg-elisa-kit-ab274339.pdf

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Microbiology Organism Virus RNA Virus ssRNA positive strand virus SARS Coronavirus
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COVID-19 N-Protein Human IgG ELISA Kit (ab274339)

  • Datasheet
  • SDS
  • Protocol Booklet
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Example data

    Key features and details

    • Range: 1.37 U/ml - 1000 U/ml
    • Sample type: Serum
    • Detection method: Colorimetric
    • Assay type: Quantitative

    Overview

    • Product name

      COVID-19 N-Protein Human IgG ELISA Kit
      See all COVID-19 N-Protein kits
    • Detection method

      Colorimetric
    • Sample type

      Serum
    • Assay type

      Quantitative
    • Range

      1.37 U/ml - 1000 U/ml
    • Assay duration

      Multiple steps standard assay
    • Product overview

      COVID-19 N-Protein Human IgG ELISA Kit (ab274339) is an in vitro indirect ELISA for the quantitative measurement of human IgG antibody against SARS-CoV-2 N protein in human serum.


      Standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARS-CoV-2 N protein, which combines with the corresponding antibody present in a sample and Positive Control which used as calibration curve for interpretation purposes. The wells are washed, and biotinylated anti-human IgG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells and color develops in proportion to the amount of COVID19 N protein human IgG antibody bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.


      The Positive Controls are from an inactivated serum sample which contains SARS-COV-2 N protein human IgG antibody. We do not know the exact amount of SARS-COV-2 N protein human IgG antibody in the Positive Control sample. The Positive Control can be used as calibration curve for interpretation purposes in different assays.

    • Notes

      Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
      It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.

    • Platform

      Pre-coated microplate (12 x 8 well strips)

    Properties

    • Storage instructions

      Store at -80°C. Please refer to protocols.
    • Components 1 x 96 tests
      20X Wash Buffer 1 x 25ml
      5X Assay Diluent B 1 x 15ml
      5X Sample Diluent 1 x 25ml
      Biotinylated anti-Human IgG Antibody 1 vial
      HRP-Streptavidin Concentrate 1 vial
      Positive Control 1 vial
      SARS-CoV-2 N protein coated Microplate 1 unit
      Stop Solution 1 x 8ml
      TMB One-Step Substrate Reagent 1 x 12ml
    • Research areas

      • Microbiology
      • Organism
      • Virus
      • RNA Virus
      • ssRNA positive strand virus
      • SARS Coronavirus

    Images

    • Example data
      Example data

      Example Positive Control calibration curve.

    Protocols

    • Protocol Booklet

    Click here to view the general protocols

    Datasheets and documents

    • SDS download

    • Datasheet download

      Download

    References (0)

    Publishing research using ab274339? Please let us know so that we can cite the reference in this datasheet.

    ab274339 has not yet been referenced specifically in any publications.

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