Key features and details
- Range: 1.37 U/ml - 1000 U/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Product nameCOVID-19 S-Protein (S1RBD) Human IgG ELISA Kit
See all COVID-19 Spike Protein kits
Range1.37 U/ml - 1000 U/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
COVID-19 S-Protein (S1RBD) Human IgG ELISA Kit (ab274340) is an in vitro indirect ELISA for the quantitative measurement of human IgG antibody against SARSCoV-2 S1 RBD protein in human serum.
Standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARS-CoV-2 S1 RBD protein, which combines with the corresponding antibody present in a sample and Positive Control, which used as calibration curve for interpretation purposes. The wells are washed, and biotinylated anti-human IgG antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells, and color develops in proportion to the amount of COVID19 S1 RBD protein human IgG antibody bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The Positive Controls are from an inactivated serum sample which contains SARS-COV-2 S1 RBD protein human IgG antibody. We do not know the exact amount of SARS-COV-2 S1 RBD protein human IgG antibody in the Positive Control sample. The Positive Control can be used as a calibration curve for interpretation purposes in different assays.
Tested applicationsSuitable for: ELISAmore details
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -20°C. Please refer to protocols.
Components 1 x 96 tests SARS-CoV-2 S1 RBD protein coated Microplate 1 unit 20X Wash Buffer Concentrate 1 x 25ml Positive Control 1 vial Biotinylated Anti-Human IgG 2 vials HRP-Streptavidin Concentrate 1 vial TMB One-Step Substrate Reagent 1 x 12ml Stop Solution 1 x 8ml 5X Assay Diluent B 1 x 15ml 5X Sample Diluent 1 x 25ml
Our Abpromise guarantee covers the use of ab274340 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use at an assay dependent concentration.|
ab274340 has not yet been referenced specifically in any publications.