Key features and details
- Range: 1.37 U/ml - 1000 U/ml
- Sample type: Serum
- Detection method: Colorimetric
- Assay type: Quantitative
- Reacts with: Human
Product nameCOVID-19 S-Protein (S1RBD) Human IgM ELISA Kit
See all COVID-19 Spike Protein kits
Range1.37 U/ml - 1000 U/ml
Assay durationMultiple steps standard assay
Species reactivityReacts with: Human
COVID-19 S-Protein (S1RBD) Human IgM ELISA Kit (ab276186) is an in vitro indirect ELISA for the quantitative measurement of human IgM antibody against SARSCoV-2 S1 RBD protein in human serum or dried blood.
Standard 96-well plates (12 strips with 8 wells/strip) are coated with the SARS-CoV-2 S1 RBD protein, which combines with the corresponding antibody present in a sample and Positive Control, which used as calibration curve for interpretation purposes. The wells are washed, and biotinylated anti-human IgM antibody is added. After washing away unbound biotinylated antibody, HRP-conjugated streptavidin is pipetted to the wells. The wells are again washed, a TMB substrate solution is added to the wells, and color develops in proportion to the amount of COVID19 S1 RBD protein human IgM antibody bound. The Stop Solution changes the color from blue to yellow, and the intensity of the color is measured at 450 nm.
The Positive Control is from an inactivated serum sample or a dried blood sample which contains SARS-COV-2 S1 RBD protein human IgM antibody. We do not know the exact amount of SARS-COV-2 S1 RBD protein human IgM antibody in the Positive Control sample. The Positive Control can be used as a calibration curve for interpretation purposes in different assays.
Abcam has not and does not intend to apply for the REACH Authorisation of customers’ uses of products that contain European Authorisation list (Annex XIV) substances.
It is the responsibility of our customers to check the necessity of application of REACH Authorisation, and any other relevant authorisations, for their intended uses.
PlatformPre-coated microplate (12 x 8 well strips)
Storage instructionsStore at -80°C. Please refer to protocols.
Components 1 x 96 tests 20X Wash Buffer 2 x 25ml 5X Assay Diluent B 1 x 15ml 5X Sample Diluent 1 x 25ml Albumin protein coated 96 well-Microplate 1 unit Biotinylated anti-human IgM antibody 2 vials HRP-Streptavidin Concentrate 1 vial Positive Control 2 vials SARS-CoV-2 S1 RBD protein coated Microplate 1 unit Stop Solution 1 x 16ml TMB One-Step Substrate Reagent 1 x 24ml
ab276186 has not yet been referenced specifically in any publications.