Overview

  • Product name

    Anti-Cowdria Ruminantium antibody [1E5H8]
    See all Cowdria Ruminantium primary antibodies
  • Description

    Mouse monoclonal [1E5H8] to Cowdria Ruminantium
  • Host species

    Mouse
  • Tested applications

    Suitable for: ELISA, WBmore details
  • Species reactivity

    Reacts with: Cow
  • Immunogen

    Elementary bodies of the mouse-pathogeninic Welgevonden isolate of Cowdria.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
  • Storage buffer

    Constituent: Tris buffered saline

    0.2um filtered
  • Concentration information loading...
  • Purity

    Protein G purified
  • Clonality

    Monoclonal
  • Clone number

    1E5H8
  • Isotype

    IgG2b
  • Light chain type

    kappa
  • Research areas

Applications

Our Abpromise guarantee covers the use of ab72231 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA Use at an assay dependent dilution.
WB Use at an assay dependent dilution. Predicted molecular weight: 32 kDa.

Target

  • Relevance

    Heartwater, Cowdria ruminantium, is an infectious rickettsial disease of domestic and wild ruminants, spread by ticks. Affected include cattle, sheep, goats, antelope and buffalo. The disease’s name is derived from the fact that fluid collects around the heart or in the lungs of infected animals.
  • Alternative names

    • Cowdria ruminantium 32kD protein antibody
    • Heartwater antibody

Images

  • Western blot of extracts of umbilical endothelial cell cultures infected with three stocks of C. ruminantium probed with MAbs.

    Lanes 1 to 5 contain antigen of Cowdria (Senegal).
    Lane 1: positive anti-Cowdria (Senegal) goat serum,
    Lane 2: Mab IE3H10,
    Lane 3: ab72231,
    Lane 4: Mab 4F10B4,
    Lane 5: Mab 3D8H1.
    Lanes 6 to 10 contain antigen of the Cowdria strain Welgevonden.
    Lane 6: positive anti-Cowdria (Welgevonden) goat serum,
    Lane 7: Mab I E3HI0,
    Lane 8: Mab IE5H8,
    Lane 9: Mab 4F10B4,
    Lane 10: Mab 3D8H1.
    Lanes 11 to 15 contain Cowdria antigen.
    Lane 11: positive anti-Cowdria goat serum,
    Lane 12: Mab IE3HI0,
    Lane 13: Mab lE5H8,
    Lane 14: Mab ab72104,
    Lane 15: Mab 3D8H1.

    Molecular mass markers in kD are indicated on the left.

References

This product has been referenced in:

  • Jongejan F  et al. Competitive enzyme-linked immunosorbent assay for heartwater using monoclonal antibodies to a Cowdria ruminantium-specific 32-kilodalton protein. Vet Microbiol 28:199-211 (1991). Read more (PubMed: 1871954) »
See 1 Publication for this product

Customer reviews and Q&As

Question
Answer

Thank you for your inquiry.

I am happy to confirm that we do offer two antibodies against Ehrlichia ruminantium (formerly Cowdria ruminantium) that belongs to Rickettsia bacteria:

ab72104

https://www.abcam.com/index.html?datasheet=72104 (or use the following: https://www.abcam.com/index.html?datasheet=72104).

ab72231

https://www.abcam.com/index.html?datasheet=72231 (or use the following: https://www.abcam.com/index.html?datasheet=72231).

I hope this in formation is helpful and wish you good luck with your research.

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