Anti-COX IV antibody [20E8C12] (ab14744)
- Datasheet
- References (187)
- Protocols
Overview
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Product nameAnti-COX IV antibody [20E8C12]
See all COX IV primary antibodies -
DescriptionMouse monoclonal [20E8C12] to COX IV
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Host speciesMouse
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Tested applicationsSuitable for: Flow Cyt, IHC-FoFr, WB, ICC/IF, IHC-Fr, IHC-P, IPmore details
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Species reactivityReacts with: Mouse, Rat, Hamster, Cow, Human, Pig, Drosophila melanogaster, Zebrafish
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Immunogen
Tissue, cells or virus corresponding to Cow COX IV.
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Positive control
- Human, bovine, murine and rat heart mitochondria.
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General notes
This antibody clone is manufactured by Abcam.
This antibody makes an effective loading control for mitochondria. COXIV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COXIV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size. Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations. An alternative mitochondrial loading control is Rabbit polyclonal to COX IV antibody (ab16056).
If you require this antibody in a particular buffer formulation or a particular conjugate for your experiments, please contact orders@abcam.com or you can find further information here.
Properties
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FormLiquid
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Storage instructionsShipped at 4°C. Store at +4°C.
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Storage bufferPreservative: 0.02% Sodium azide
Constituent: HEPES buffered saline -
Concentration information loading... -
PurityIgG fraction
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Primary antibody notesThis antibody makes an effective loading control for mitochondria. COXIV is generally expressed at a consistent high level. However, be aware that many proteins run at the same 16kD size as COXIV - our VDAC1 / Porin antibody makes a good alternative mitochondrial loading control for proteins of this size. Some caution is required when using this antibody as a loading control as COXIV expression can vary under some manipulations. An alternative mitochondrial loading control is Rabbit polyclonal to COX IV antibody (ab16056).
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ClonalityMonoclonal
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Clone number20E8C12
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IsotypeIgG2a
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Light chain typekappa
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Research areas
Associated products
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Alternative Versions
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Compatible Secondaries
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Immunohistochemistry kits
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Isotype control
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Related Products
Applications
Our Abpromise guarantee covers the use of ab14744 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt | Use 0.5µg for 106 cells. ab170191 - Mouse monoclonal IgG2a, is suitable for use as an isotype control with this antibody. |
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| IHC-FoFr | Use at an assay dependent concentration. PubMed: 18698340 | |
| WB | Use a concentration of 1 µg/ml. Detects a band of approximately 17 kDa. | |
| ICC/IF | Use at an assay dependent concentration. | |
| IHC-Fr | Use at an assay dependent concentration. | |
| IHC-P | Use at an assay dependent concentration. | |
| IP | Use at an assay dependent concentration. |
Target
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FunctionThis protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
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Tissue specificityUbiquitous.
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Sequence similaritiesBelongs to the cytochrome c oxidase IV family.
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Cellular localizationMitochondrion inner membrane.
- Information by UniProt
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Database links
- Entrez Gene: 1327 Human
- Entrez Gene: 12857 Mouse
- Entrez Gene: 29445 Rat
- Entrez Gene: 326975 Zebrafish
- Omim: 123864 Human
- SwissProt: P00423 Cow
- SwissProt: P13073 Human
- SwissProt: P19783 Mouse
see all -
Alternative names
- AL024441 antibody
- COX 4 antibody
- COX IV 1 antibody
see all
Images
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![Western blot - Anti-COX IV antibody [20E8C12] (ab14744)](https://www.abcam.com/ps/products/14/ab14744/Images/ab14744-197882-ab14744wb.jpg)
Western blot - Anti-COX IV antibody [20E8C12] (ab14744)This image is courtesy of an anonymous AbreviewAnti-COX IV antibody [20E8C12] (ab14744) at 1/1000 dilution + Fruit fly (Drosophila melanogaster) heart tube tissue lysate at 10 µg
Secondary
HRP-conjugate Goat anti-mouse IgG polyclonal at 1/4000 dilution
Developed using the ECL technique.
Performed under reducing conditions.
Exposure time: 1 minute
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![Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [20E8C12] (ab14744)](https://www.abcam.com/ps/products/14/ab14744/Images/ab14744_7.jpg)
Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody [20E8C12] (ab14744)This image is courtesy of an anonymous Abreviewab14744 at 1/100 staining MCF-10A cells (human mammary epithelial cell line) by ICC/IF. The cells were methanol/acetone fixed at -20C for 5 minutes, blocked with BSA and then incubated with the antibody for 16 hours. The positive tissue was colocalised with a mitotracker (mitotrackers are a series of patented mt-selective stains that are concentrated by active mt and well retained during cell fixation). An Alexa-Fluor ® 488 conjugated goat anti-mouse antibody was used as the secondary. The image shows COXIV staining in green and DAPI staining in blue.
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![Flow Cytometry - Anti-COX IV antibody [20E8C12] (ab14744)](https://www.abcam.com/ps/products/14/ab14744/Images/COX-IV-Primary-antibodies-ab14744-15.jpg)
Flow Cytometry - Anti-COX IV antibody [20E8C12] (ab14744)Overlay histogram showing HeLa cells stained with ab14744 (red line). The cells were fixed with 80% methanol (5 min) and then permeabilized with 0.1% PBS-Tween for 20 min. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab14744, 0.5µg/1x106 cells) for 30 min at 22°C. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 min at 22°C. Isotype control antibody (black line) was mouse IgG1 [ICIGG1](ab91353, 2µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed. -
![Western blot - Anti-COX IV antibody [20E8C12] (ab14744)](https://www.abcam.com/ps/products/14/ab14744/Images/ab14744_1.jpg)
Western blot - Anti-COX IV antibody [20E8C12] (ab14744)All lanes : Anti-COX IV antibody [20E8C12] (ab14744) at 1/5000 dilution
Lane 1 : Cytoplasmic fraction mouse NIH/3T3 cell lysate
Lane 2 : Nuclear fraction mouse NIH/3T3 cell lysate
Secondary
All lanes : HRP conjugated goat anti-mouse antibody
Developed using the ECL technique.
Performed under reducing conditions.
Observed band size: 16 kDa why is the actual band size different from the predicted?
Exposure time: 3 minutes
This image is courtesy of an Abreview submitted by Camilla Skjerpen on 4 July 2005. -
![Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-COX IV antibody [20E8C12] (ab14744)](https://www.abcam.com/ps/products/14/ab14744/Images/COX-IV-Primary-antibodies-ab14744-7.jpg)
Immunohistochemistry (PFA perfusion fixed frozen sections) - Anti-COX IV antibody [20E8C12] (ab14744)Image from Hashimoto T. et. al., PLoS ONE. 2008; 3(8): e2915 (Fig. 1B)ab14744 staining COX IV in rat brain tissue sections by Immunohistochemistry (PFA perfusion fixed frozen sections). Rats were anesthetized and intracardially perfused with 500 ml of normal saline at room temperature, followed by 500 ml of ice-cold, freshly made 4% paraformaldehyde in phosphate buffer (PB, 0.1 M, pH 7.4). A Cy3 conjugated anti mouse antibody was used as secondary.
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![Western blot - Anti-COX IV antibody [20E8C12] (ab14744)](https://www.abcam.com/ps/products/14/ab14744/Images/COX-IV-Primary-antibodies-ab14744-11.jpg)
Western blot - Anti-COX IV antibody [20E8C12] (ab14744)All lanes : Anti-COX IV antibody [20E8C12] (ab14744) at 1 µg/ml
Lane 1 : Isolated mitochondria from human heart at 5 µg
Lane 2 : Isolated mitochondria from bovine heart at 1 µg
Lane 3 : Isolated mitochondria from rat heart at 10 µg
Lane 4 : Isolated mitochondria from murine heart at 10 µg
Observed band size: 16 kDa why is the actual band size different from the predicted?
Protocols
References
This product has been referenced in:
- Zuo W et al. Hyperglycemia abolished Drp-1-mediated mitophagy at the early stage of cerebral ischemia. Eur J Pharmacol 843:34-44 (2019). Read more (PubMed: 30447185) »
- Signes A et al. APOPT1/COA8 assists COX assembly and is oppositely regulated by UPS and ROS. EMBO Mol Med 11:N/A (2019). Read more (PubMed: 30552096) »
