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I am still hunting for some decent antibodies that I can use to verify findings by Western. I am interested in polyclonal Abs that react with Human and Mouse cell lines against membrane bound proteins of Nuclei and Mitochondria . I want to make sure that the proteins used to generate the Abs are not soluble. I have not had any luck using the polyclonal Nesprin Ab from you guys. I get more of a ladder with HeLa lysate rather than a specific 112 kDa band. The same is true for the polyclonal TOM22 Ab that gives me a band at 60 kDa rather than the specific band at 18 kDa.
Can you please suggest some Nuc and Mito Abs that are targeted against membrane bound proteins? These would preferably be polyclonal and react with both human and mouse. And the application would be for western, where there is one specific band.
Asked on Sep 12 2012
Thank you very much for your enquiry.
I am sorry to hear that the nesprin and TOMM22 antibodies are not working as they should be. Could you tell me the catalog numbers of each of these antibodies, and also a description of the protocol that you're using? We do fully guarantee all of our products to work as stated on the datasheets, so I'd be happy to send replacements if applicable. Could you also send your original order or PO number?
If you'd like to continue probing for nesprin and TOMM22, we do have a couple of antibodies that work in Western blot with human and mouse samples-
We also have several other antibodies that are targeted against mitochondrial or nuclear membrane-bound proteins that work in Western blot with mouse and human samples:
Prohibition antibody: https://www.abcam.com/Prohibitin-antibody-Mitochondrial-Marker-ab28172.html
MTCO1 antibody: https://www.abcam.com/MTCO1-antibody-1D6E1A8-Mitochondrial-Marker-ab14705.html
Lamin B1 antibodies: https://www.abcam.com/Lamin-B1-antibody-Nuclear-Envelope-Marker-ab16048.html
Would one of these work for you?
I look forward to hearing from you. Please let me know if you have any questions or if there is anything else that we can do for you.
Answered on Sep 12 2012