Western blot abreview for Anti-COX IV antibody [20E8C12] - Mitochondrial Loading Control

Excellent
Abreviews
Application
Western blot
Sample
Human Cell lysate - other (isolated mitochondria from HaCaT cells)
Loading amount
20 µg
Specification
isolated mitochondria from HaCaT cells
Treatment
50mJ/cm2 UV treatment
Gel Running Conditions
Reduced Denaturing
Blocking step
Milk as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C

Other product details

Dilution
1/1000
Incubation time
16 hour(s) and 0 minute(s) · Temperature: 4°C

Secondary antibody

Name
Non-Abcam antibody was used: Goat anti-mouse HRP
Host species: Goat
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/5000

Detection

Detection method
SuperSignal ® West PiCo
Exposure
20 second(s)
Bands
Specific: 17 kDa

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Submitted Jul 14 2011

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