Immunocytochemistry/ Immunofluorescence abreview for Anti-COX IV antibody [20E8C12] - Mitochondrial Loading Control

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Mouse Cell (colonic epithelial cells)
Specification
colonic epithelial cells
Fixative
Paraformaldehyde
Permeabilization
Yes - Saponin 0.5%
Blocking step
BSA as blocking agent for 45 minute(s) · Concentration: 1% · Temperature: 20°C

Other product details

Dilution
1/20
Incubation time
2 hour(s) and 0 minute(s) · Temperature: 20°C · Diluent: Saponin 0.5% BSA 1%

Secondary antibody

Name
Non-Abcam antibody was used: Alexa Fluor® 488 chicken anti-mouse IgG
Host species: Chicken
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488
Dilution
1/200

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Submitted Dec 07 2011

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