Western blot abreview for Anti-COX IV antibody [20E8C12] - Mitochondrial Loading Control

Good
Abreviews
Application
Western blot
Sample
Mouse Cell lysate - other (NIH/3T3 cells)
Specification
NIH/3T3 cells
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 2 %

Other product details

Dilution
1/5000
Incubation time
2 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: anti-mouse
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL
Exposure
3 minute(s) and 0 second(s)
Bands
Specific: 16 kDa
Positive control
Cells were fractionated into different fractions. Only in he cytoplasmic fraction (1) and not in the nuclear fraction (2) could a band be detected when this antibody was used.

Additional data

Additional Notes
The antibody gave one band in cell lysates form NIH/3T3 cells. The blot was clear and easily detectable.

Camilla Skjerpen

Verified customer

Submitted Jul 04 2005

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