Western blot abreview for Anti-COX IV [20E8C12] antibody

Excellent
Abreviews
Application
Western blot
Loading amount
50 µg
Gel Running Conditions
Reduced Denaturing (8-12)
Sample
Mouse Tissue lysate - whole (liver)
Specification
liver
Blocking step
No blocking step used for 30 minute(s) · Temperature: 30°C

Other product details

Incubation time
15 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: Licor Odyssey block + 0.1% Tween 20
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: Licor Goat anti-mouse 680 IR dye
Host species: Goat
Clonality: Polyclonal
Conjugation: IRDye­ 680
Dilution
1/2000

Detection

Detection method
Licor
Bands
Specific: ~15 kDa
Exposure
2 minute(s) and 0 second(s)
Positive control
Samples were liver tissue taken from P9 neonatal mice

Dr. Gillian Hunter

Verified customer

Submitted Aug 06 2013

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