Western blot abreview for Anti-COX IV [20E8C12] antibody

Average
Abreviews
Application
Western blot
Loading amount
100000 cells
Gel Running Conditions
Reduced Denaturing (10%)
Sample
Human Cell lysate - whole cell (Jurkats)
Specification
Jurkats
Treatment
TCR activation with OKT3 and anti CD28
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 3% · Temperature: 25°C

Other product details

Incubation time
14 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: 3% BSA
Dilution
1/1000

Secondary antibody

Name
Non-Abcam antibody was used: chicken anti mouse
Host species: Chicken
Clonality: Monoclonal
Conjugation: Horse Radish Peroxidase
Dilution
1/1000

Detection

Detection method
ECL
Bands
Specific: ~15 kDa
Exposure
30 second(s)

Dr. Tejpal Gill

Verified customer

Submitted Jan 13 2014

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