Western blot abreview for Anti-COX IV antibody [20E8C12]

Good
Abreviews
Application
Western blot
Sample
Sheep Cell lysate - other (preadipocyte mitochondrial isolates v. cytoplasmic)
Gel Running Conditions
Reduced Denaturing (10% PAG)
Loading amount
10 µg
Specification
preadipocyte mitochondrial isolates v. cytoplasmic
Blocking step
Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 23°C

Other product details

Incubation time
18 hour(s) and 0 minute(s) · Temperature: 4°C · Diluent: milk/TBS
Dilution
1/800

Secondary antibody

Dilution
1/5000
Name
Non-Abcam antibody was used: goat anti-rabbit HRP conjugate
Host species: Goat
Clonality: Polyclonal
Conjugation: Horse Radish Peroxidase

Detection

Detection method
ECL+
Negative control
cytoplasmic isolates, lane 3/4
Exposure
20 second(s)
Bands
Specific: 17 kDa
Positive control
mitochondrial isolates, lane 1/2

Additional data

Additional Notes
mitochondrial isolates, lane 1/2 cytoplasmic isolates, lane 3/4 Partial lysate retained in loading well

Miss. Barbara Makela

Verified customer

Submitted Oct 13 2016

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