Immunocytochemistry/ Immunofluorescence abreview for Anti-COX IV antibody [20E8C12] - Mitochondrial Loading Control

Excellent
Abreviews
Application
Immunocytochemistry/ Immunofluorescence
Sample
Human Cell (MCF-10A, human mammary epithelial cell line)
Specification
MCF-10A, human mammary epithelial cell line
Fixative
Methanol
Blocking step
BSA as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 4%

Other product details

Dilution
1/100
Incubation time
16 hour(s) and 0 minute(s)

Secondary antibody

Name
Non-Abcam antibody was used: goat anti-mouse secondary antibody (Invitrogen)
Host species: Goat
Clonality: Polyclonal
Conjugation: Alexa Fluor® 488

Additional data

Additional Notes
fixation in methanol/acetone 1:1 at -20C for 5 min
Image legend: blue = DAPI and green = COX IV.

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Submitted Feb 12 2007

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