Product nameAnti-COX IV antibody [EPR9442(ABC)] (HRP)
See all COX IV primary antibodies
DescriptionRabbit monoclonal [EPR9442(ABC)] to COX IV (HRP)
Tested applicationsSuitable for: IHC-P, WBmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human COX IV aa 1-100. The exact sequence is proprietary.
Database link: P13073
- WB: HepG2 whole cell and Mouse Heart tissue and Rat Heart tissue lysates IHC-P: normal human colon tissue sections
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMab® patents.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Storage bufferpH: 7.40
Preservative: 0.1% 10% Proclin 300 Solution
Constituents: 1% BSA, 30% Glycerol, PBS
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Energy transfer pathways
- Integration of energy
- Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)
- Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (Alexa Fluor® 405) (ab210180)
- Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (Alexa Fluor® 594) (ab210674)
- Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (Alexa Fluor® 555) (ab210675)
- Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (Alexa Fluor® 568) (ab210848)
- Anti-COX IV antibody [EPR9442(ABC)] - BSA and Azide free (ab231168)
Our Abpromise guarantee covers the use of ab209958 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|IHC-P||1/100. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.|
|WB||1/5000. Detects a band of approximately 17 kDa (predicted molecular weight: 20 kDa).|
FunctionThis protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
Sequence similaritiesBelongs to the cytochrome c oxidase IV family.
Cellular localizationMitochondrion inner membrane.
- Information by UniProt
- AL024441 antibody
- COX 4 antibody
- COX IV 1 antibody
IHC image of COX IV staining in a section of formalin-fixed paraffin-embedded normal human colon*, performed on a Leica BOND™. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH6, epitope retrieval solution 1) for 20mins. The section was then incubated with ab209958, 1/100 dilution, for 15 mins at room temperature. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX. The inset negative control image is taken from an identical assay without primary antibody.
For other IHC staining systems (automated and non-automated) customers should optimize variable parameters such as antigen retrieval conditions, primary antibody concentration and antibody incubation times.
*Tissue obtained from the Human Research Tissue Bank, supported by the NIHR Cambridge Biomedical Research Centre
All lanes : Anti-COX IV antibody [EPR9442(ABC)] (HRP) (ab209958) at 1/5000 dilution
Lane 1 : HepG2 (Human hepatocellular liver carcinoma cell line) Whole Cell Lysate
Lane 2 : Heart (Mouse) Tissue Lysate
Lane 3 : Heart (Rat) Tissue Lysate
Lysates/proteins at 10 µg per lane.
Developed using the ECL technique.
Performed under reducing conditions.
Predicted band size: 20 kDa
Observed band size: 17 kDa why is the actual band size different from the predicted?
Exposure time: 5 seconds
This blot was produced using a 4-12% Bis-tris gel under the MES buffer system. The gel was run at 200V for 35 minutes before being transferred onto a Nitrocellulose membrane at 30V for 70 minutes. The membrane was then blocked for an hour using 2% Bovine Serum Albumin before being incubated with ab209958 overnight at 4°C. Antibody binding was visualised using ECL development solution ab133406.
ab209958 has not yet been referenced specifically in any publications.