Western blot abreview for Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control

Good
Abreviews
Application
Western blot
Sample
Pig Tissue lysate - other (mitochondria)
Gel Running Conditions
Reduced Denaturing (12% BIS/Tris)
Loading amount
20 µg
Specification
mitochondria
Blocking step
Commercial block solution as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 100% · Temperature: 20°C

Other product details

Incubation time
19 hour(s) and 0 minute(s) · Temperature: 20°C
Dilution
1/1000

Secondary antibody

Dilution
1/2000
Name
Non-Abcam antibody was used: ECL plex goat-anti-rabbit IgG Cy5
Host species: Goat
Clonality: Polyclonal
Conjugation: Cy5 ­

Detection

Detection method
Fluorescence
Negative control
None
Exposure
1 hour(s), 30 minute(s) and 0 second(s)
Positive control
Human heart mitochondria lysate ab110337 (10ug) Observed band at ~17kDa

Additional data

Additional Notes
Lane 1 = 10 ug Pig brain mitochondria lysate Lane 2 = 15 ug Pig brain mitochondria lysate Lane 3 = 20 ug Pig brain mitochondria lysate Lane 4 = 10 ug human heart mitochondria lysate

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Submitted Mar 01 2017

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