Anti-COX IV antibody - Mitochondrial Marker (ab153709)
Key features and details
- Rabbit polyclonal to COX IV - Mitochondrial Marker
- Suitable for: WB, IHC-P, ICC/IF
- Reacts with: Mouse, Rat, Human
- Isotype: IgG
Overview
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Product name
Anti-COX IV antibody - Mitochondrial Marker
See all COX IV primary antibodies -
Description
Rabbit polyclonal to COX IV - Mitochondrial Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Monkey
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Immunogen
Recombinant fragment within Human COX IV aa 1-169. The exact sequence is proprietary.
Database link: P13073 -
Positive control
- WB: HepG2 whole cell lysate (ab7900), mouse and rat muscle tissue lysate. IHC-P: Human HS578T xenograft tissue. ICC/IF: HeLa cells.
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General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin 300
Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine) -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab153709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 19 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the cytochrome c oxidase IV family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 1327 Human
- Entrez Gene: 12857 Mouse
- Entrez Gene: 29445 Rat
- Omim: 123864 Human
- SwissProt: P13073 Human
- SwissProt: P19783 Mouse
- SwissProt: P10888 Rat
- Unigene: 433419 Human
see all -
Alternative names
- AL024441 antibody
- COX 4 antibody
- COX IV 1 antibody
see all
Images
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Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709)Anti-COX IV antibody - Mitochondrial Marker (ab153709) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 19 kDa
12% SDS PAGE -
Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709)Anti-COX IV antibody - Mitochondrial Marker (ab153709) at 1/500 dilution + Mouse muscle whole tissue lysate at 50 µg
Predicted band size: 19 kDa
12% SDS PAGE -
Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709)Anti-COX IV antibody - Mitochondrial Marker (ab153709) at 1/500 dilution + Rat muscle whole tissue lysate at 50 µg
Predicted band size: 19 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Marker (ab153709)Immunohistochemical analysis of paraffin embedded HS578T (human) xenograft tissue, labeling COX IV using ab153709 at a 1/250 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Marker (ab153709)Immunofluorescence analysis of methanol fixed HeLa cells, labeling COX IV using ab153709 at 1/500 dilution (green) and alpha tubulin filaments (red).
Protocols
References (5)
ab153709 has been referenced in 5 publications.
- Suo D et al. PM2.5 induces apoptosis, oxidative stress injury and melanin metabolic disorder in human melanocytes. Exp Ther Med 19:3227-3238 (2020). PubMed: 32269607
- Kowluru RA & Mohammad G Epigenetics and Mitochondrial Stability in the Metabolic Memory Phenomenon Associated with Continued Progression of Diabetic Retinopathy. Sci Rep 10:6655 (2020). PubMed: 32313015
- Livingston MJ et al. Clearance of damaged mitochondria via mitophagy is important to the protective effect of ischemic preconditioning in kidneys. Autophagy 15:2142-2162 (2019). PubMed: 31066324
- Gao X et al. Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway. Mol Med Rep 16:515-522 (2017). IHC ; Rat . PubMed: 28560462
- Panfoli I et al. Exosomes from human mesenchymal stem cells conduct aerobic metabolism in term and preterm newborn infants. FASEB J 30:1416-24 (2016). PubMed: 26655706
