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    cox-iv-antibody-mitochondrial-marker-ab153709.pdf

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Tags & Cell Markers Subcellular Markers Organelles Mitochondria
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Anti-COX IV antibody - Mitochondrial Marker (ab153709)

  • Datasheet
  • SDS
Submit a review Q&A (1)References (5)

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Find out more.

Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709)
  • Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709)
  • Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709)
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Marker (ab153709)
  • Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Marker (ab153709)

Key features and details

  • Rabbit polyclonal to COX IV - Mitochondrial Marker
  • Suitable for: WB, IHC-P, ICC/IF
  • Reacts with: Mouse, Rat, Human
  • Isotype: IgG

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Anti-COX IV antibody [EPR9442(ABC)] - Mitochondrial Loading Control (ab202554)

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Overview

  • Product name

    Anti-COX IV antibody - Mitochondrial Marker
    See all COX IV primary antibodies
  • Description

    Rabbit polyclonal to COX IV - Mitochondrial Marker
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WB, IHC-P, ICC/IFmore details
  • Species reactivity

    Reacts with: Mouse, Rat, Human
    Predicted to work with: Monkey
  • Immunogen

    Recombinant fragment within Human COX IV aa 1-169. The exact sequence is proprietary.
    Database link: P13073

  • Positive control

    • WB: HepG2 whole cell lysate (ab7900), mouse and rat muscle tissue lysate. IHC-P: Human HS578T xenograft tissue. ICC/IF: HeLa cells.
  • General notes

    Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.

    Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.

    We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.

    In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.

    We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.

    Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.

    Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.

Properties

  • Form

    Liquid
  • Storage instructions

    Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle.
  • Storage buffer

    pH: 7.00
    Preservative: 0.025% Proclin 300
    Constituents: 78% PBS, 1% BSA, 20% Glycerol (glycerin, glycerine)
  • Concentration information loading...
  • Purity

    Immunogen affinity purified
  • Clonality

    Polyclonal
  • Isotype

    IgG
  • Research areas

    • Tags & Cell Markers
    • Subcellular Markers
    • Organelles
    • Mitochondria
    • Signal Transduction
    • Metabolism
    • Mitochondrial
    • Isotype/Loading Controls
    • Loading Controls
    • COX IV - Mitochondrial
    • Cancer
    • Cancer Metabolism
    • Metabolic signaling pathway
    • Integration of energy metabolism
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Mitochondrial markers
    • Metabolism
    • Pathways and Processes
    • Metabolic signaling pathways
    • Energy transfer pathways
    • Integration of energy
    • Metabolism
    • Pathways and Processes
    • Mitochondrial Metabolism
    • Oxidative phosphorylation
    • Complex IV

Associated products

  • Compatible Secondaries

    • Goat Anti-Rabbit IgG H&L (Alexa Fluor® 488) (ab150077)
    • Goat Anti-Rabbit IgG H&L (HRP) (ab205718)
  • Isotype control

    • Rabbit IgG, polyclonal - Isotype Control (ChIP Grade) (ab171870)
  • Recombinant Protein

    • Recombinant Human COX IV protein (ab112273)
  • Related Products

    • Recombinant Human COX IV protein (ab112273)

Applications

Our Abpromise guarantee covers the use of ab153709 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB 1/500 - 1/3000. Predicted molecular weight: 19 kDa.
IHC-P 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol.
ICC/IF 1/100 - 1/1000.

Target

  • Function

    This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the cytochrome c oxidase IV family.
  • Cellular localization

    Mitochondrion inner membrane.
  • Target information above from: UniProt accession P13073 The UniProt Consortium
    The Universal Protein Resource (UniProt) in 2010
    Nucleic Acids Res. 38:D142-D148 (2010) .

    Information by UniProt
  • Database links

    • Entrez Gene: 1327 Human
    • Entrez Gene: 12857 Mouse
    • Entrez Gene: 29445 Rat
    • Omim: 123864 Human
    • SwissProt: P13073 Human
    • SwissProt: P19783 Mouse
    • SwissProt: P10888 Rat
    • Unigene: 433419 Human
    • Unigene: 386758 Mouse
    • Unigene: 2528 Rat
    see all
  • Alternative names

    • AL024441 antibody
    • COX 4 antibody
    • COX IV 1 antibody
    • COX IV antibody
    • COX IV-1 antibody
    • Cox4 antibody
    • COX41_HUMAN antibody
    • Cox4a antibody
    • COX4B antibody
    • COX4I1 antibody
    • COX4I2 antibody
    • COX4L2 antibody
    • COXIV antibody
    • Cytochrome c oxidase polypeptide IV antibody
    • Cytochrome c oxidase subunit 4 isoform 1 mitochondrial antibody
    • Cytochrome c oxidase subunit 4 isoform 1, mitochondrial antibody
    • Cytochrome C Oxidase subunit IV antibody
    • Cytochrome c oxidase subunit IV isoform 1 antibody
    • Cytochrome c oxidase subunit IV isoform 2 (lung) antibody
    • Cytochrome c oxydase subunit 4 antibody
    • dJ857M17.2 antibody
    • MGC105470 antibody
    • MGC72016 antibody
    see all

Images

  • Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709)
    Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709)
    Anti-COX IV antibody - Mitochondrial Marker (ab153709) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg

    Predicted band size: 19 kDa



    12% SDS PAGE
  • Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709)
    Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709)
    Anti-COX IV antibody - Mitochondrial Marker (ab153709) at 1/500 dilution + Mouse muscle whole tissue lysate at 50 µg

    Predicted band size: 19 kDa



    12% SDS PAGE
  • Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709)
    Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709)
    Anti-COX IV antibody - Mitochondrial Marker (ab153709) at 1/500 dilution + Rat muscle whole tissue lysate at 50 µg

    Predicted band size: 19 kDa



    12% SDS PAGE
  • Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Marker (ab153709)
    Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Marker (ab153709)

    Immunohistochemical analysis of paraffin embedded HS578T (human) xenograft tissue, labeling COX IV using ab153709 at a 1/250 dilution.

  • Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Marker (ab153709)
    Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Marker (ab153709)
    Immunofluorescence analysis of methanol fixed HeLa cells, labeling COX IV using ab153709 at 1/500 dilution (green) and alpha tubulin filaments (red).

Protocols

  • Immunohistochemistry protocols
  • Immunocytochemistry & immunofluorescence protocols
  • Western blot protocols

Click here to view the general protocols

Datasheets and documents

    • Datasheet
    • SDS
  • References (5)

    Publishing research using ab153709? Please let us know so that we can cite the reference in this datasheet.

    ab153709 has been referenced in 5 publications.

    • Suo D  et al. PM2.5 induces apoptosis, oxidative stress injury and melanin metabolic disorder in human melanocytes. Exp Ther Med 19:3227-3238 (2020). PubMed: 32269607
    • Kowluru RA & Mohammad G Epigenetics and Mitochondrial Stability in the Metabolic Memory Phenomenon Associated with Continued Progression of Diabetic Retinopathy. Sci Rep 10:6655 (2020). PubMed: 32313015
    • Livingston MJ  et al. Clearance of damaged mitochondria via mitophagy is important to the protective effect of ischemic preconditioning in kidneys. Autophagy 15:2142-2162 (2019). PubMed: 31066324
    • Gao X  et al. Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway. Mol Med Rep 16:515-522 (2017). IHC ; Rat . PubMed: 28560462
    • Panfoli I  et al. Exosomes from human mesenchymal stem cells conduct aerobic metabolism in term and preterm newborn infants. FASEB J 30:1416-24 (2016). PubMed: 26655706

    Customer reviews and Q&As

    Show All Reviews Q&A
    Submit a review Submit a question

    Question

    IHC-Fr, will this work? Special incubation?

    Read More

    Abcam community

    Verified customer

    Asked on Jan 14 2013

    Answer

    Vielen Dank für Ihren Anruf und für Ihr Interesse an unseren Produkten.

    Unseres Wissens nach wurde ab153709 Anti-COX IV antibody bisher noch nicht in IHC-Fr getestet. Falls Sie dies selbst testen möchten, kann ich Ihnen zur Zeit ein spezielles Angebot über einen 100%igen Abreview-Rabatt anbieten. Bei diesem Angebot bekommen Sie einen Rabatt für eine zukünftige Bestellung, wenn Sie uns ein Abreview mit dem Testresultat zusenden.


    Um von diesem Angebot profitieren zu können, folgen Sie bitte diesen Schritten:

    1.) Bestätigen Sie mir bitte, dass Sie ab153709 in IHC-Fr testen möchten.

    2.) Bitte bestellen Sie erst nach Erhalt des Rabattcodes.

    3.) Bestellen Sie den Antikörper wie üblich per Telefon, Email oder Fax

    4.) Testen Sie den Antikörper in IHC-Fr.
    5.) Teilen Sie uns das Ergebnis Ihres Tests durch ein Abreview mit und notieren Sie den Discount Code in dem Feld "additional notes"
    Unter der folgenden URL können Sie mehr über unser Abreview System erfahren:
    https://www.abcam.com/abreviews

    6.) Der Rabattcode ist nach dem Abschicken des Abreviews aktiv. Bitte beachten Sie, dass der Rabattcode innerhalb von 4 Monaten nach Ausstellung eingelöst werden muss.

    Der Rabattcode wird gültig unabhängig davon, ob Ihr Ergebnis positiv oder negativ ist.

    Die Bedingungen zu unserem 100% Abreview Rabatt können Sie unter dem folgenden Link nachlesen:
    https://www.abcam.com/collaborationdiscount

    Ich hoffe, diese Informationen helfen Ihnen weiter. Falls Sie einen Rabattcode erhalten möchten oder weitere Fragen haben, zögern Sie bitte nicht, sich wieder an mich zu wenden.

    Read More

    Abcam Scientific Support

    Answered on Jan 14 2013

    Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
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