Anti-COX IV antibody - Mitochondrial Marker (ab153709)
- Datasheet
- References (3)
- Protocols
Overview
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Product name
Anti-COX IV antibody - Mitochondrial Marker
See all COX IV primary antibodies -
Description
Rabbit polyclonal to COX IV - Mitochondrial Marker -
Host species
Rabbit -
Tested applications
Suitable for: WB, IHC-P, ICC/IFmore details -
Species reactivity
Reacts with: Mouse, Rat, Human
Predicted to work with: Monkey
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Immunogen
Recombinant fragment within Human COX IV aa 1-169. The exact sequence is proprietary.
Database link: P13073 -
Positive control
- WB: HepG2 whole cell lysate (ab7900), mouse and rat muscle tissue lysate. IHC-P: Human HS578T xenograft tissue. ICC/IF: HeLa cells.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot. Store at -20°C or -80°C. Avoid freeze / thaw cycle. -
Storage buffer
pH: 7.00
Preservative: 0.025% Proclin
Constituents: PBS, 1% BSA, 20% Glycerol -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Positive Controls
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Recombinant Protein
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Related Products
Applications
Our Abpromise guarantee covers the use of ab153709 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | 1/500 - 1/3000. Predicted molecular weight: 19 kDa. | |
| IHC-P | 1/100 - 1/1000. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. | |
| ICC/IF | 1/100 - 1/1000. |
Target
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Function
This protein is one of the nuclear-coded polypeptide chains of cytochrome c oxidase, the terminal oxidase in mitochondrial electron transport. -
Tissue specificity
Ubiquitous. -
Sequence similarities
Belongs to the cytochrome c oxidase IV family. -
Cellular localization
Mitochondrion inner membrane. - Information by UniProt
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Database links
- Entrez Gene: 1327 Human
- Entrez Gene: 12857 Mouse
- Entrez Gene: 29445 Rat
- Omim: 123864 Human
- SwissProt: P13073 Human
- SwissProt: P19783 Mouse
- SwissProt: P10888 Rat
- Unigene: 433419 Human
see all -
Alternative names
- AL024441 antibody
- COX 4 antibody
- COX IV 1 antibody
see all
Images
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Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709)Anti-COX IV antibody - Mitochondrial Marker (ab153709) at 1/1000 dilution + HepG2 whole cell lysate at 30 µg
Predicted band size: 19 kDa
12% SDS PAGE -
Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709)Anti-COX IV antibody - Mitochondrial Marker (ab153709) at 1/500 dilution + Mouse muscle whole tissue lysate at 50 µg
Predicted band size: 19 kDa
12% SDS PAGE -
Western blot - Anti-COX IV antibody - Mitochondrial Marker (ab153709)Anti-COX IV antibody - Mitochondrial Marker (ab153709) at 1/500 dilution + Rat muscle whole tissue lysate at 50 µg
Predicted band size: 19 kDa
12% SDS PAGE -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX IV antibody - Mitochondrial Marker (ab153709)Immunohistochemical analysis of paraffin embedded HS578T (human) xenograft tissue, labeling COX IV using ab153709 at a 1/250 dilution.
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Immunocytochemistry/ Immunofluorescence - Anti-COX IV antibody - Mitochondrial Marker (ab153709)Immunofluorescence analysis of methanol fixed HeLa cells, labeling COX IV using ab153709 at 1/500 dilution (green) and alpha tubulin filaments (red).
Protocols
References
This product has been referenced in:
- Livingston MJ et al. Clearance of damaged mitochondria via mitophagy is important to the protective effect of ischemic preconditioning in kidneys. Autophagy 15:2142-2162 (2019). Read more (PubMed: 31066324) »
- Gao X et al. Remote ischemic postconditioning confers neuroprotective effects via inhibition of the BID-mediated mitochondrial apoptotic pathway. Mol Med Rep 16:515-522 (2017). IHC ; Rat . Read more (PubMed: 28560462) »
