Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)
![Flow Cytometry - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)](https://www.abcam.com/ps/products/0/ab695/Images/ab695-3642-anti-cox1-cyclooxygenase-1-antibody-5f6-f4-flow-cytometry.jpg "Flow Cytometry - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)")
Key features and details
- Mouse monoclonal [5F6/F4] to COX1 / Cyclooxygenase 1
- Suitable for: Flow Cyt
- Reacts with: Mouse
- Isotype: IgG1
Overview
-
Product name
Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4]
See all COX1 / Cyclooxygenase 1 primary antibodies -
Description
Mouse monoclonal [5F6/F4] to COX1 / Cyclooxygenase 1 -
Host species
Mouse -
Tested Applications & Species
See all applications and species dataApplication Species Flow Cyt Mouse
-
Immunogen
Other Immunogen Type corresponding to COX1/ Cyclooxygenase 1. A preparation of intracellular proteins of the human cell line HL60.
-
Positive control
- WB: Huh-7 whole cell lysate. COX1 protein standard prepared from ram seminal vesicles. Flow Cytometry: NIH/3T3 cells. IHC-P: Human anal cancer tissue. Mouse kidney tissue. IHC-Fr: Human breast and breast tumor tissue. IP: Huh-7 whole cell lysate.
-
General notes
Please note that we have received reports from customers working with this antibody that they obtain two stong bands in Western blot. A single band at around 72kDa is expected. One customer reported that when tested with three different human cell lines two strong bands are obtained, one between 40 - 50kDa and other other between 62 - 83kDa. It is possible that a degraded form of the enzyme is detected or the bands may correspond to different glycosylation states.
Properties
-
Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles. -
Storage buffer
Preservative: 0.02% Sodium azide
FCS free -
Concentration information loading... -
Purity
Immunogen affinity purified -
Clonality
Monoclonal -
Clone number
5F6/F4 -
Isotype
IgG1 -
Light chain type
kappa -
Research areas
Associated products
-
Assay kits
-
Compatible Secondaries
-
Conjugation kits
-
Isotype control
-
Recombinant Protein
Applications
The Abpromise guarantee
Our Abpromise guarantee covers the use of ab695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
Tested applications are guaranteed to work and covered by our Abpromise guarantee.
Predicted to work for this combination of applications and species but not guaranteed.
Does not work for this combination of applications and species.
| Application | Species |
|---|---|
| Flow Cyt |
Mouse
|
| Application | Abreviews | Notes |
|---|---|---|
| Flow Cyt |
Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
| Notes |
|---|
|
Flow Cyt
Use 1-2µg for 106 cells. ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|
Target
-
Function
May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells. -
Pathway
Lipid metabolism; prostaglandin biosynthesis. -
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
-
Database links
- Entrez Gene: 19224 Mouse
- SwissProt: P22437 Mouse
- Unigene: 275434 Mouse
-
Alternative names
- COX 1 antibody
- COX 3 antibody
- COX-1 antibody
see all
Images
-
Flow Cytometry - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)
Overlay histogram showing NIH/3T3 (Mouse embryo fibroblast cell line) cells stained with ab695 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab695, 2 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
Protocols
References (17)
ab695 has been referenced in 17 publications.
- Lim SC et al. Extracellular acidity-induced expression of Kallikrein-related peptidases 7 and 8 is involved in increased invasiveness of gastric cancer cells. Oncol Rep 43:1705-1713 (2020). PubMed: 32323843
- Korczyñska-Krawczyk P et al. The effect of levocetirizine and montelukast on clinical symptoms, serum level and skin expression of COX-1 and COX-2 enzymes in patients suffering from chronic autoimmune urticaria - a pilot study. Postepy Dermatol Alergol 37:73-80 (2020). PubMed: 32467688
- Lim SC et al. Ellagic Acid Inhibits Extracellular Acidity-Induced Invasiveness and Expression of COX1, COX2, Snail, Twist 1, and c-myc in Gastric Carcinoma Cells. Nutrients 11:N/A (2019). PubMed: 31835645
- Chen WY et al. Androgen deprivation-induced ZBTB46-PTGS1 signaling promotes neuroendocrine differentiation of prostate cancer. Cancer Lett 440-441:35-46 (2019). PubMed: 30312731
- Xiao M et al. Impact of the Chinese herbal medicines on dual antiplatelet therapy with clopidogrel and aspirin: Pharmacokinetics and pharmacodynamics outcomes and related mechanisms in rats. J Ethnopharmacol 235:100-110 (2019). PubMed: 30710735
