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    cox1--cyclooxygenase-1-antibody-5f6f4-ab695.pdf

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Immunology Innate Immunity Macrophage / Inflamm.
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Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)

  • Datasheet
  • SDS
Reviews (5)Q&A (1)References (17)

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Flow Cytometry - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)

    Key features and details

    • Mouse monoclonal [5F6/F4] to COX1 / Cyclooxygenase 1
    • Suitable for: Flow Cyt
    • Reacts with: Mouse
    • Isotype: IgG1

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    Secondary
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    View more associated products

    Overview

    • Product name

      Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4]
      See all COX1 / Cyclooxygenase 1 primary antibodies
    • Description

      Mouse monoclonal [5F6/F4] to COX1 / Cyclooxygenase 1
    • Host species

      Mouse
    • Tested Applications & Species

      Application Species
      Flow Cyt
      Mouse
      See all applications and species data
    • Immunogen

      Other Immunogen Type corresponding to COX1/ Cyclooxygenase 1. A preparation of intracellular proteins of the human cell line HL60.

    • Positive control

      • WB: Huh-7 whole cell lysate. COX1 protein standard prepared from ram seminal vesicles. Flow Cytometry: NIH/3T3 cells. IHC-P: Human anal cancer tissue. Mouse kidney tissue. IHC-Fr: Human breast and breast tumor tissue. IP: Huh-7 whole cell lysate.
    • General notes

      Please note that we have received reports from customers working with this antibody that they obtain two stong bands in Western blot. A single band at around 72kDa is expected. One customer reported that when tested with three different human cell lines two strong bands are obtained, one between 40 - 50kDa and other other between 62 - 83kDa. It is possible that a degraded form of the enzyme is detected or the bands may correspond to different glycosylation states.

    Properties

    • Form

      Liquid
    • Storage instructions

      Shipped at 4°C. Upon delivery aliquot and store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
    • Storage buffer

      Preservative: 0.02% Sodium azide

      FCS free
    • Concentration information loading...
    • Purity

      Immunogen affinity purified
    • Clonality

      Monoclonal
    • Clone number

      5F6/F4
    • Isotype

      IgG1
    • Light chain type

      kappa
    • Research areas

      • Immunology
      • Innate Immunity
      • Macrophage / Inflamm.
      • Cardiovascular
      • Blood
      • Platelets
      • Signal Transduction
      • Signaling Pathway
      • Lipid Signaling
      • Prostaglandins
      • Cardiovascular
      • Atherosclerosis
      • Lipoprotein metabolism
      • Cancer
      • Cancer Metabolism
      • Metabolic signaling pathway
      • Hormone biosynthesis
      • Metabolism
      • Pathways and Processes
      • Metabolic signaling pathways
      • Lipid and lipoprotein metabolism
      • Lipoprotein metabolism
      • Metabolism
      • Pathways and Processes
      • Redox metabolism
      • Oxidative stress
      • Metabolism
      • Pathways and Processes
      • Endocrine metabolism
      • Hormone biosynthesis
      • Metabolism
      • Types of disease
      • Cancer
      • Metabolism
      • Types of disease
      • Heart disease

    Associated products

    • Assay kits

      • Cyclooxygenase (COX) Activity Assay Kit (Fluorometric) (ab204699)
    • Compatible Secondaries

      • Goat Anti-Mouse IgG H&L (Alexa Fluor® 488) (ab150113)
      • Goat Anti-Mouse IgG H&L (HRP) (ab205719)
      • Goat Anti-Mouse IgG H&L (DyLight® 488) preadsorbed (ab96879)
    • Conjugation kits

      • PE / R-Phycoerythrin Conjugation Kit - Lightning-Link® (ab102918)
      • APC Conjugation Kit - Lightning-Link® (ab201807)
    • Isotype control

      • Mouse IgG1, kappa monoclonal [15-6E10A7] - Isotype Control (ab170190)
      • Mouse IgG1, Kappa Monoclonal [B11/6] - Isotype Control (ab91353)
    • Recombinant Protein

      • Recombinant human COX1 / Cyclooxygenase 1 protein (ab198643)

    Applications

    The Abpromise guarantee

    Our Abpromise guarantee covers the use of ab695 in the following tested applications.

    The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

    Guaranteed

    Tested applications are guaranteed to work and covered by our Abpromise guarantee.

    Predicted

    Predicted to work for this combination of applications and species but not guaranteed.

    Incompatible

    Does not work for this combination of applications and species.

    Application Species
    Flow Cyt
    Mouse
    Application Abreviews Notes
    Flow Cyt
    Use 1-2µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Notes
    Flow Cyt
    Use 1-2µg for 106 cells.

    ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.

     

    Target

    • Function

      May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
    • Pathway

      Lipid metabolism; prostaglandin biosynthesis.
    • Sequence similarities

      Belongs to the prostaglandin G/H synthase family.
      Contains 1 EGF-like domain.
    • Cellular localization

      Microsome membrane. Endoplasmic reticulum membrane.
    • Target information above from: UniProt accession P23219 The UniProt Consortium
      The Universal Protein Resource (UniProt) in 2010
      Nucleic Acids Res. 38:D142-D148 (2010) .

      Information by UniProt
    • Database links

      • Entrez Gene: 19224 Mouse
      • SwissProt: P22437 Mouse
      • Unigene: 275434 Mouse
      • Alternative names

        • COX 1 antibody
        • COX 3 antibody
        • COX-1 antibody
        • COX1 antibody
        • Cox3 antibody
        • Cyclooxygenase 1 antibody
        • Cyclooxygenase 3, included antibody
        • Cyclooxygenase-1 antibody
        • EC 1.14.99.1 antibody
        • Partial COX1 proteins, included antibody
        • PCOX1 antibody
        • PGG/HS antibody
        • PGH synthase 1 antibody
        • PGH1_HUMAN antibody
        • PGHS 1 antibody
        • PGHS-1 antibody
        • PGHS1 antibody
        • PHS 1 antibody
        • PHS1 antibody
        • Prostaglandin endoperoxide synthase 1 antibody
        • Prostaglandin G/H synthase 1 antibody
        • Prostaglandin H2 synthase 1 antibody
        • Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) antibody
        • Prostaglandin-endoperoxide synthase 1 antibody
        • PTGHS antibody
        • PTGS1 antibody
        see all

      Images

      • Flow Cytometry - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)
        Flow Cytometry - Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4] (ab695)

        Overlay histogram showing NIH/3T3 (Mouse embryo fibroblast cell line) cells stained with ab695 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab695, 2 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.

      Protocols

      • Flow cytometry protocols
      • Immunocytochemistry & immunofluorescence protocols

      Click here to view the general protocols

      Datasheets and documents

      • Datasheet
      • SDS
    • References (17)

      Publishing research using ab695? Please let us know so that we can cite the reference in this datasheet.

      ab695 has been referenced in 17 publications.

      • Lim SC  et al. Extracellular acidity-induced expression of Kallikrein-related peptidases 7 and 8 is involved in increased invasiveness of gastric cancer cells. Oncol Rep 43:1705-1713 (2020). PubMed: 32323843
      • Korczyñska-Krawczyk P  et al. The effect of levocetirizine and montelukast on clinical symptoms, serum level and skin expression of COX-1 and COX-2 enzymes in patients suffering from chronic autoimmune urticaria - a pilot study. Postepy Dermatol Alergol 37:73-80 (2020). PubMed: 32467688
      • Lim SC  et al. Ellagic Acid Inhibits Extracellular Acidity-Induced Invasiveness and Expression of COX1, COX2, Snail, Twist 1, and c-myc in Gastric Carcinoma Cells. Nutrients 11:N/A (2019). PubMed: 31835645
      • Chen WY  et al. Androgen deprivation-induced ZBTB46-PTGS1 signaling promotes neuroendocrine differentiation of prostate cancer. Cancer Lett 440-441:35-46 (2019). PubMed: 30312731
      • Xiao M  et al. Impact of the Chinese herbal medicines on dual antiplatelet therapy with clopidogrel and aspirin: Pharmacokinetics and pharmacodynamics outcomes and related mechanisms in rats. J Ethnopharmacol 235:100-110 (2019). PubMed: 30710735
      View all Publications for this product

      Customer reviews and Q&As

      Show All Reviews Q&A
      Submit a review Submit a question

      1-6 of 6 Abreviews or Q&A

      Immunoprecipitation abreview for Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunoprecipitation
      Sample
      Human Cell lysate - whole cell (huh-7)
      Total protein in input
      1e+006 cells
      Specification
      huh-7
      Treatment
      cells expressing recombitant myc-tagged COX1
      Immuno-precipitation step
      Protein A
      Read More

      Abcam user community

      Verified customer

      Submitted Jun 18 2010

      Western blot abreview for Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (huh-7 cell line)
      Loading amount
      15 µg
      Specification
      huh-7 cell line
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 16 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 4°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 27 2009

      Immunoprecipitation abreview for Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Immunoprecipitation
      Sample
      Human Cell lysate - whole cell (293T)
      Total protein in input
      200 µg
      Specification
      293T
      Immuno-precipitation step
      Protein G
      Read More

      Abcam user community

      Verified customer

      Submitted Dec 07 2007

      Western blot abreview for Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4]

      Excellent
      Abreviews
      Abreviews
      abreview image
      Application
      Western blot
      Sample
      Human Cell lysate - whole cell (U2OS and 293T cell line)
      Loading amount
      30 µg
      Specification
      U2OS and 293T cell line
      Gel Running Conditions
      Reduced Denaturing
      Blocking step
      Milk as blocking agent for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: 21°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 07 2007

      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) abreview for Anti-COX1 / Cyclooxygenase 1 antibody [5F6/F4]

      Good
      Abreviews
      Abreviews
      abreview image
      Application
      Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
      Sample
      Human Tissue sections (anal cancer)
      Specification
      anal cancer
      Fixative
      Formaldehyde
      Antigen retrieval step
      Heat mediated - Buffer/Enzyme Used: Citrate Buffer pH 6.0
      Permeabilization
      No
      Blocking step
      Serum as blocking agent for 30 minute(s) · Concentration: 5% · Temperature: 22°C
      Read More

      Abcam user community

      Verified customer

      Submitted Nov 02 2007

      Question

      Is this antibody stable at low pH (2.8)?

      Read More

      Abcam community

      Verified customer

      Asked on Feb 19 2007

      Answer

      Thank you for your enquiry. I am happy to forward on the reply from the originator of the antibody, which I hope you will find helpful. "Normally IgG antibodies are purified using elution buffers at lower pH of 2-3. During this purification the antibodies are stable and reactive after changing the solution to ph ~ 7. It should be possible to use ab695 under the described conditions." I hope this information helps. Please do not hesitate to contact us if you need anything further.

      Read More

      Abcam Scientific Support

      Answered on Feb 20 2007

      Please note: All products are "FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES"
      For licensing inquiries, please contact partnerships@abcam.com

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