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Other Immunogen Type corresponding to COX1/ Cyclooxygenase 1. A preparation of intracellular proteins of the human cell line HL60.
Our Abpromise guarantee covers the use of ab695 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ELISA||Use a concentration of 0.001 - 0.01 µg/ml.|
|WB||Use a concentration of 1 - 2 µg/ml.|
|IHC-Fr||Use a concentration of 10 µg/ml.|
|IHC-P||Use at an assay dependent concentration.|
|Flow Cyt||Use 1-2µg for 106 cells.
ab170190 - Mouse monoclonal IgG1, is suitable for use as an isotype control with this antibody.
|IP||Use at an assay dependent concentration.|
|ICC/IF||Use at an assay dependent concentration.|
Primary antibody incubated for 1 hour at 20°C in 5% milk in TBST. Gel running conditions: Denaturing. Blocked using 5% milk for 16 hours at 4°C. Detection method: Western lightning chemiluminescent reagent.
Human adjacent breast tissue to tumor or breast tumor tissue labeled by indirect immunofluorescence for COX-1 (red) using ab695, with DAPI as nuclear counterstain (blue).
ab695 at 1/400 staining human anal cancer tissue (Left panel) and mouse kidney tissue (Right panel) sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed before the tissue was incubated with the antibody for 45 minutes. An HRP conjugated goat anti-mouse antibody was used as the secondary.
Overlay histogram showing NIH/3T3 (Mouse embryo fibroblast cell line) cells stained with ab695 (red line). The cells were fixed with 80% methanol (5 minutes) and then permeabilized with 0.1% PBS-Tween for 20 minutes. The cells were then incubated in 1x PBS / 10% normal goat serum / 0.3M glycine to block non-specific protein-protein interactions followed by the antibody (ab695, 2 µg/1x106 cells) for 30 minutes at 22ºC. The secondary antibody used was DyLight® 488 goat anti-mouse IgG (H+L) (ab96879) at 1/500 dilution for 30 minutes at 22ºC. Isotype control antibody (black line) was mouse IgG1 [ICIGG1] (ab91353, 2 µg/1x106 cells) used under the same conditions. Acquisition of >5,000 events was performed.
COX1 protein standard prepared from ram seminal vesicles supplied by Alpha Diagnostic International.
Lane 1 MM.
Lane 2 Sample
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