Question (46458) | Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)

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Question

Hi,

I have recently purchased the ab109025 Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] and would like to the know any suggestion for the use of high temperature mediated antigen retrieval solution – citrate (pH 6) or high pH (pH 9) suitable for formalin fixed paraffin embedded sections?

Also, is there any problem of condensation involved if I want to aliquot the antibody which is in glycerol?

Best Regards,

Answer

Thank you for your inquiry.

I am happy toconfirm that the image on the datasheet was produced using Citrate buffer pH6 for antigen retrieval. Therefore I suggest to also use this buffer in your experiments with high temperature.

The exact protocol is not available and has to be optimized according to your experimental set-up and samples.

Since this antibody is stored in glycerol and should not freeze through, the only advantage of aliquoting is the contamination prevention. It is therefore not necessary to have very small aliquots and condensation or evaporation should not make problems in this case.

I hope this information is helpful and wish you good luck with your research.

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