Key features and details
- Produced recombinantly (animal-free) for high batch-to-batch consistency and long term security of supply
- Rabbit monoclonal [EPR5866] to COX1 / Cyclooxygenase 1 (Alexa Fluor® 647)
- Suitable for: ICC/IF
- Reacts with: Mouse, Rat, Human
- Conjugation: Alexa Fluor® 647. Ex: 652nm, Em: 668nm
Product nameAnti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (Alexa Fluor® 647)
See all COX1 / Cyclooxygenase 1 primary antibodies
DescriptionRabbit monoclonal [EPR5866] to COX1 / Cyclooxygenase 1 (Alexa Fluor® 647)
ConjugationAlexa Fluor® 647. Ex: 652nm, Em: 668nm
Tested applicationsSuitable for: ICC/IFmore details
Species reactivityReacts with: Mouse, Rat, Human
Synthetic peptide within Human COX1/ Cyclooxygenase 1 aa 100-200. The exact sequence is proprietary.
- NIH3T3, HACAT, Neuro -2a, C2C12, A431, and L6 cell lysates; Human skin tissue; HeLa cells. ICC/IF: HeLa cells
Our RabMAb® technology is a patented hybridoma-based technology for making rabbit monoclonal antibodies. For details on our patents, please refer to RabMAb® patents.
Alexa Fluor® is a registered trademark of Molecular Probes, Inc, a Thermo Fisher Scientific Company. The Alexa Fluor® dye included in this product is provided under an intellectual property license from Life Technologies Corporation. As this product contains the Alexa Fluor® dye, the purchase of this product conveys to the buyer the non-transferable right to use the purchased product and components of the product only in research conducted by the buyer (whether the buyer is an academic or for-profit entity). As this product contains the Alexa Fluor® dye the sale of this product is expressly conditioned on the buyer not using the product or its components, or any materials made using the product or its components, in any activity to generate revenue, which may include, but is not limited to use of the product or its components: in manufacturing; (ii) to provide a service, information, or data in return for payment (iii) for therapeutic, diagnostic or prophylactic purposes; or (iv) for resale, regardless of whether they are sold for use in research. For information on purchasing a license to this product for purposes other than research, contact Life Technologies Corporation, 5781 Van Allen Way, Carlsbad, CA 92008 USA or email@example.com.
Storage instructionsShipped at 4°C. Store at +4°C short term (1-2 weeks). Upon delivery aliquot. Store at -20°C. Stable for 12 months at -20°C. Store In the Dark.
Dissociation constant (KD)KD = 5.50 x 10 -12 M Learn more about KD
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 30% Glycerol, PBS, 1% BSA
Concentration information loading...
PurityProtein A purified
- Pathways and Processes
- Metabolic signaling pathways
- Lipid and lipoprotein metabolism
- Lipoprotein metabolism
- Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (ab109025)
- Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (Alexa Fluor® 488) (ab199027)
- Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (HRP) (ab199203)
- Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] (PE) (ab209581)
- Anti-COX1 / Cyclooxygenase 1 antibody [EPR5866] - BSA and Azide free (ab219375)
Our Abpromise guarantee covers the use of ab199202 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
This product gave a positive signal in 100% methanol (5 min)
FunctionMay play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
PathwayLipid metabolism; prostaglandin biosynthesis.
Sequence similaritiesBelongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain.
Cellular localizationMicrosome membrane. Endoplasmic reticulum membrane.
- Information by UniProt
- COX 1 antibody
- COX 3 antibody
- COX-1 antibody
ab199202 staining COX1 / Cyclooxygenase 1 in HeLa cells. The cells were fixed with 100% methanol (5min), permeabilized with 0.1% Triton X-100 for 5 minutes and then blocked with 1% BSA/10% normal goat serum/0.3M glycine in 0.1% PBS-Tween for 1h. The cells were then incubated overnight at +4°C with ab199202 at 1/1000 dilution (shown in red) and ab195887, Mouse monoclonal to alpha Tubulin (Alexa Fluor® 488), at 1/250 dilution (shown in green). Nuclear DNA was labelled with DAPI (shown in blue).
Image was taken with a confocal microscope (Leica-Microsystems, TCS SP8).
To our knowledge, customised protocols are not required for this product. Please try the standard protocols listed below and let us know how you get on.
ab199202 has not yet been referenced specifically in any publications.