Overview

  • Product name

    Anti-COX1 / Cyclooxygenase 1 antibody
    See all COX1 / Cyclooxygenase 1 primary antibodies
  • Description

    Rabbit polyclonal to COX1 / Cyclooxygenase 1
  • Host species

    Rabbit
  • Tested applications

    Suitable for: WBmore details
  • Species reactivity

    Reacts with: Human
  • Immunogen

    Synthetic peptide corresponding to Human COX1/ Cyclooxygenase 1 aa 550 to the C-terminus (C terminal) conjugated to keyhole limpet haemocyanin.
    (Peptide available as ab93495)

  • Positive control

    • This antibody gave a positive signal in the following human whole cell lysates: A431; HUVEC.

Properties

Applications

Our Abpromise guarantee covers the use of ab84602 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
WB
  • Application notes
    WB: Use at a concentration of 1 µg/ml. Detects a band of approximately 73 kDa (predicted molecular weight: 68 kDa).
    Not yet tested in other applications.
    Optimal dilutions/concentrations should be determined by the end user.
  • Target

    • Function

      May play an important role in regulating or promoting cell proliferation in some normal and neoplastically transformed cells.
    • Pathway

      Lipid metabolism; prostaglandin biosynthesis.
    • Sequence similarities

      Belongs to the prostaglandin G/H synthase family.
      Contains 1 EGF-like domain.
    • Cellular localization

      Microsome membrane. Endoplasmic reticulum membrane.
    • Information by UniProt
    • Database links

    • Alternative names

      • COX 1 antibody
      • COX 3 antibody
      • COX-1 antibody
      • COX1 antibody
      • Cox3 antibody
      • Cyclooxygenase 1 antibody
      • Cyclooxygenase 3, included antibody
      • Cyclooxygenase-1 antibody
      • EC 1.14.99.1 antibody
      • Partial COX1 proteins, included antibody
      • PCOX1 antibody
      • PGG/HS antibody
      • PGH synthase 1 antibody
      • PGH1_HUMAN antibody
      • PGHS 1 antibody
      • PGHS-1 antibody
      • PGHS1 antibody
      • PHS 1 antibody
      • PHS1 antibody
      • Prostaglandin endoperoxide synthase 1 antibody
      • Prostaglandin G/H synthase 1 antibody
      • Prostaglandin H2 synthase 1 antibody
      • Prostaglandin-endoperoxide synthase 1 (prostaglandin G/H synthase and cyclooxygenase) antibody
      • Prostaglandin-endoperoxide synthase 1 antibody
      • PTGHS antibody
      • PTGS1 antibody
      see all

    Images

    • All lanes : Anti-COX1 / Cyclooxygenase 1 antibody (ab84602) at 1 µg/ml

      Lane 1 : A431 (Human epithelial carcinoma cell line) Whole Cell Lysate
      Lane 2 : HUVEC (Human Umbilical Vein Endothelial Cell) Whole Cell Lysate

      Lysates/proteins at 10 µg per lane.

      Secondary
      All lanes : Goat polyclonal to Rabbit IgG - H&L - Pre-Adsorbed (HRP) at 1/3000 dilution

      Developed using the ECL technique.

      Performed under reducing conditions.

      Predicted band size: 68 kDa
      Observed band size: 71,73 kDa
      why is the actual band size different from the predicted?
      Additional bands at: 55 kDa. We are unsure as to the identity of these extra bands.


      Exposure time: 2 minutes


      This antibody was raised against an immunogen that is predicted to recognize both the short and long isofroms of Human Prostaglandin G/H synthase 1 precursor (COX1). The predicted molecular weights of the short and long isoforms are 68 kDa and 64 kDa respectively. However, COX1 contains a number of potential glycosylation sites (SwissProt) which may explain its migration at a higher molecular weight than predicted.

    References

    ab84602 has not yet been referenced specifically in any publications.

    Customer reviews and Q&As

    1-2 of 2 Abreviews or Q&A

    Answer

    Thank you for your reply.

    Indeed, the other antibodies mentioned are only tested for WB so far, however, they are the antibodies against these targets that have the highest probability to cross-react with hamster due to sequence similarity of the immunogen. This means that other antibodies that we have available against these target might work in IHC, but they are less likely to cross-react with hamster. We do not see a reason why ab84602, ab66770, ab58139, and ab104705 should not work in IHC - it has just not been tested yet. Therefore, I can issue you discount codes for these as well, if you would like to test them also in IHC.

    Unfortunately, Abcam does not ship directly to India, however we do have a distributor who you can contact regarding the price information. Please find more information depending on your local region on our website (Central and South: Allied Scientific Products, North: NuLife Consultants & Distributors (Pvt.) Ltd.).


    DISCOUNT CODE for ab102005: xx
    Expiration date: dd mm 2012

    I am very pleased to hear you would like to accept our offer and test ab102005 in hamster. This code will give you 1 free primary antibody before the expiration date. To redeem this offer, please submit an Abreview for hamster and include this code in the “Additional Comments” section so we know the Abreview is for this promotion. For more information on how to submit an Abreview, please visit the site: www.abcam.com/Abreviews

    Remember, we publish both positive and negative Abreviews on our datasheets so please submit the results of your tests. The code will be active once the Abreview has been submitted and can be redeemed in one of the following ways: 1) Call to place your order and mention the code to our customer service department; 2) Include the code in your fax order; 3) Place your order on the web and enter the promotional code.

    Any feedback that you can provide will be greatly appreciated, whether positive or negative. If you have any further questions, please do not hesitate to contact us. We look forward to receiving your Abreview and wish you luck with your research.

    The terms and conditions applicable to this offer can be found here: www.abcam.com/collaborationdiscount.

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    Answer

    Thank you very much for contacting us.

    I am happy to let you know that we have the following antibodies available:

    COX-2: ab102005: Click here (or use the following: https://www.abcam.com/index.html?datasheet=102005).
    COX-1: ab84602: Click here (or use the following: https://www.abcam.com/index.html?datasheet=84602).
    LIF: ab104705: Click here (or use the following: https://www.abcam.com/index.html?datasheet=104705).
    PPAR delta: ab66770: Click here (or use the following: https://www.abcam.com/index.html?datasheet=66770).
    PPAR delta: ab58139: Click here (or use the following: https://www.abcam.com/index.html?datasheet=58139).

    Our Abpromise® ensures that you can trust our products to perform as stated on the datasheets, or we will offer a replacement, credit, or refund (www.abcam.com/Abpromise). To our knowledge, these antibodies have not been tested in hamster yet. However, based on a BLAST search the immunogens exhibit a sequence homology of 84-88% with the hamster ortholog and therefore the antibodies might very well cross-react. Therefore, I can offer a discount off a future purchase if you buy one or more of these antibodies now, test it in hamster and/or IHC and submit feedback to us in the form of an Abreview. It doesn’t matter whether the Abreview is positive or negative, we would just really like to receive your feedback. The discount would be to the value of 1 free primary antibody each.

    If you are interested in this offer, please follow these steps:

    1. Reply to this e-mail to let me know that you would like to proceed and which antibody you would like to test in hamster and/or IHC. I will then send a discount code for each antibody. This codes must be issued before purchasing the antibody so please wait for my reply before ordering.

    2. Purchase the antibody either by phone, fax, or online.

    3. Test it in in hamster and/or IHC.

    4. Let us know the results, positive or negative, using our Abreview system (this will take about 10 minutes and images are great if you have them!). To find out more about our Abreview system, please visit: https://www.abcam.com/abreviews.

    5. After the review is submitted to us, the discount code becomes active. Simply place your new order by phone, fax, or on the web and mention the discount code. The discount can be redeemed for any primary antibody ordered and the discount code is valid for 4 months after issue.

    We are always pleased to obtain feedback about our products and any information is greatly appreciated! Even if the antibody turns out to be unsuitable for hamster, you will still receive the discount on your next purchase after your Abreview has been submitted.

    Please let me know if you have any questions about this offer and I would be happy to help you further.

    The Terms and Conditions of this offer can be found at: www.abcam.com/collaborationdiscount.

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