Key features and details
- Rabbit polyclonal to COX17
- Suitable for: ELISA, ICC/IF, WB, IHC-P
- Reacts with: Human
- Isotype: IgG
Product nameAnti-COX17 antibody
DescriptionRabbit polyclonal to COX17
Tested applicationsSuitable for: ELISA, ICC/IF, WB, IHC-Pmore details
Species reactivityReacts with: Human
Predicted to work with: Mouse, Rat
Synthetic peptide derived from the N-terminal of human COX17.
- Human liver carcinoma tissue. Extracts from HeLa, HepG2, 293 and COLO cells.
Storage instructionsShipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles.
Storage bufferpH: 7.40
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, 0.87% Sodium chloride, PBS
Without Mg2+ and Ca2+
Concentration information loading...
PurityImmunogen affinity purified
Our Abpromise guarantee covers the use of ab69611 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
|ICC/IF||Use a concentration of 1 µg/ml.|
|WB||1/500 - 1/1000. Detects a band of approximately <19 kDa (predicted molecular weight: 7 kDa).|
|IHC-P||1/50 - 1/100.|
FunctionCopper chaperone for cytochrome c oxidase (COX). Binds two copper ions and deliver them to the Cu(A) site of COX.
Sequence similaritiesBelongs to the COX17 family.
Cellular localizationMitochondrion intermembrane space.
- Information by UniProt
- COX 17 antibody
- COX17 antibody
- COX17 cytochrome c oxidase assembly homolog (S. cerevisiae) antibody
All lanes : Anti-COX17 antibody (ab69611) at 1/500 dilution
Lane 1 : Extracts from HeLa cells
Lane 2 : Extracts from HepG2 cells
Lane 3 : Extracts from COLO cells
Lane 4 : Extracts from 293 cells
Lane 5 : Extracts from COLO cells with immunising peptide at 5 µg
Lysates/proteins at 5 µg per lane.
Predicted band size: 7 kDa
Observed band size: <19 kDa why is the actual band size different from the predicted?
ab69611, at 1/50 dilution, staining human liver carcinoma tissue in the presence or absence of the immunising peptide.
ICC/IF image of ab69611 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69611, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.
ab69611 has been referenced in 1 publication.
- Zhong Y et al. Protective effects of apigenin against 3-MCPD-induced renal injury in rat. Chem Biol Interact 296:9-17 (2018). PubMed: 30107153