Overview

  • Product name

    Anti-COX17 antibody
  • Description

    Rabbit polyclonal to COX17
  • Host species

    Rabbit
  • Tested applications

    Suitable for: ELISA, ICC/IF, WB, IHC-Pmore details
  • Species reactivity

    Reacts with: Human
    Predicted to work with: Mouse, Rat
  • Immunogen

    Synthetic peptide derived from the N-terminal of human COX17.

  • Positive control

    • Human liver carcinoma tissue. Extracts from HeLa, HepG2, 293 and COLO cells.

Properties

Applications

Our Abpromise guarantee covers the use of ab69611 in the following tested applications.

The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.

Application Abreviews Notes
ELISA 1/5000.
ICC/IF Use a concentration of 1 µg/ml.
WB 1/500 - 1/1000. Detects a band of approximately <19 kDa (predicted molecular weight: 7 kDa).
IHC-P 1/50 - 1/100.

Target

  • Function

    Copper chaperone for cytochrome c oxidase (COX). Binds two copper ions and deliver them to the Cu(A) site of COX.
  • Tissue specificity

    Ubiquitous.
  • Sequence similarities

    Belongs to the COX17 family.
  • Cellular localization

    Mitochondrion intermembrane space.
  • Information by UniProt
  • Database links

  • Alternative names

    • COX 17 antibody
    • COX17 antibody
    • COX17 cytochrome c oxidase assembly homolog (S. cerevisiae) antibody
    • COX17 cytochrome c oxidase assembly homolog antibody
    • COX17 homolog cytochrome c oxidase assembly protein antibody
    • COX17_HUMAN antibody
    • cytochrome c oxidase assembly protein cox17 homolog antibody
    • Cytochrome c oxidase copper chaperone antibody
    • Human homolog of yeast mitochondrial copper recruitment antibody
    • MGC104397 antibody
    • MGC117386 antibody
    • OTTHUMP00000215284 antibody
    • OTTHUMP00000215285 antibody
    see all

Images

  • All lanes : Anti-COX17 antibody (ab69611) at 1/500 dilution

    Lane 1 : Extracts from HeLa cells
    Lane 2 : Extracts from HepG2 cells
    Lane 3 : Extracts from COLO cells
    Lane 4 : Extracts from 293 cells
    Lane 5 : Extracts from COLO cells with immunising peptide at 5 µg

    Lysates/proteins at 5 µg per lane.

    Predicted band size: 7 kDa
    Observed band size: <19 kDa
    why is the actual band size different from the predicted?

  • ab69611, at 1/50 dilution, staining human liver carcinoma tissue in the presence or absence of the immunising peptide.
  • ICC/IF image of ab69611 stained HeLa cells. The cells were 4% formaldehyde fixed (10 min) and then incubated in 1%BSA / 10% normal goat serum / 0.3M glycine in 0.1% PBS-Tween for 1h to permeabilise the cells and block non-specific protein-protein interactions. The cells were then incubated with the antibody (ab69611, 1µg/ml) overnight at +4°C. The secondary antibody (green) was ab96899, DyLight® 488 goat anti-rabbit IgG (H+L) used at a 1/250 dilution for 1h. Alexa Fluor® 594 WGA was used to label plasma membranes (red) at a 1/200 dilution for 1h. DAPI was used to stain the cell nuclei (blue) at a concentration of 1.43µM.

References

This product has been referenced in:

See 1 Publication for this product

Customer reviews and Q&As

Application
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections)
Sample
Rat Tissue sections (Retina)
Antigen retrieval step
Heat mediated - Buffer/Enzyme Used: sodium citrate pH 6
Permeabilization
Yes - 0.1% triton, Mg-citrate
Specification
Retina
Blocking step
(agent) for 1 hour(s) and 0 minute(s) · Concentration: 5% · Temperature: RT°C
Fixative
Paraformaldehyde

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Verified customer

Submitted Jan 03 2019

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