Anti-COX2 / Cyclooxygenase 2 antibody (ab23672)
Key features and details
- Goat polyclonal to COX2 / Cyclooxygenase 2
- Suitable for: WB, IHC-P
- Reacts with: Mouse, Rat, Sheep, Human
- Isotype: IgG
Overview
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Product name
Anti-COX2 / Cyclooxygenase 2 antibody
See all COX2 / Cyclooxygenase 2 primary antibodies -
Description
Goat polyclonal to COX2 / Cyclooxygenase 2 -
Host species
Goat -
Specificity
ab23672 recognises COX2. It does not cross react with COX1. -
Tested applications
Suitable for: WB, IHC-Pmore details -
Species reactivity
Reacts with: Mouse, Rat, Sheep, Human
Predicted to work with: Horse, Cow, Dog, Pig, Mink
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Immunogen
Synthetic peptide. This information is considered to be commercially sensitive.
(Peptide available asab49841) -
General notes
Reproducibility is key to advancing scientific discovery and accelerating scientists’ next breakthrough.
Abcam is leading the way with our range of recombinant antibodies, knockout-validated antibodies and knockout cell lines, all of which support improved reproducibility.
We are also planning to innovate the way in which we present recommended applications and species on our product datasheets, so that only applications & species that have been tested in our own labs, our suppliers or by selected trusted collaborators are covered by our Abpromise™ guarantee.
In preparation for this, we have started to update the applications & species that this product is Abpromise guaranteed for.
We are also updating the applications & species that this product has been “predicted to work with,” however this information is not covered by our Abpromise guarantee.
Applications & species from publications and Abreviews that have not been tested in our own labs or in those of our suppliers are not covered by the Abpromise guarantee.
Please check that this product meets your needs before purchasing. If you have any questions, special requirements or concerns, please send us an inquiry and/or contact our Support team ahead of purchase. Recommended alternatives for this product can be found below, as well as customer reviews and Q&As.
Properties
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Form
Liquid -
Storage instructions
Shipped at 4°C. Upon delivery aliquot and store at -20°C. Avoid freeze / thaw cycles. -
Storage buffer
pH: 7.20
Preservative: 0.02% Sodium azide
Constituents: 50% Glycerol, PBS -
Concentration information loading... -
Purity
Protein G purified -
Purification notes
ab23672 was purified by affinity chromatography. -
Clonality
Polyclonal -
Isotype
IgG -
Research areas
Associated products
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Compatible Secondaries
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Isotype control
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Recombinant Protein
Applications
Our Abpromise guarantee covers the use of ab23672 in the following tested applications.
The application notes include recommended starting dilutions; optimal dilutions/concentrations should be determined by the end user.
| Application | Abreviews | Notes |
|---|---|---|
| WB | Use a concentration of 2 µg/ml. Predicted molecular weight: 69 kDa. | |
| IHC-P | Use a concentration of 1 - 2 µg/ml. Perform heat mediated antigen retrieval with citrate buffer pH 6 before commencing with IHC staining protocol. |
Target
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Function
Mediates the formation of prostaglandins from arachidonate. May have a role as a major mediator of inflammation and/or a role for prostanoid signaling in activity-dependent plasticity. -
Pathway
Lipid metabolism; prostaglandin biosynthesis. -
Sequence similarities
Belongs to the prostaglandin G/H synthase family.
Contains 1 EGF-like domain. -
Post-translational
modificationsS-nitrosylation by NOS2 (iNOS) activates enzme activity. S-nitrosylation may take place on different Cys residues in addition to Cys-561. -
Cellular localization
Microsome membrane. Endoplasmic reticulum membrane. - Information by UniProt
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Database links
- Entrez Gene: 282023 Cow
- Entrez Gene: 5743 Human
- Entrez Gene: 19225 Mouse
- Entrez Gene: 29527 Rat
- Entrez Gene: 443460 Sheep
- Omim: 600262 Human
- SwissProt: O62698 Cow
- SwissProt: O19183 Horse
see all -
Alternative names
- COX 2 antibody
- COX-2 antibody
- COX2 antibody
see all
Images
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Western blot - Anti-COX2 / Cyclooxygenase 2 antibody (ab23672)All lanes : Anti-COX2 / Cyclooxygenase 2 antibody (ab23672) at 1/500 dilution
Lane 1 : Human COX-1
Lane 2 : Recombinant ovine COX-2 protein
Lane 3 : Recombinant human COX-2 protein
Predicted band size: 69 kDa -
Immunohistochemistry (Formalin/PFA-fixed paraffin-embedded sections) - Anti-COX2 / Cyclooxygenase 2 antibody (ab23672)Immunohistochemistry analysis of formalin fixed, paraffin-embedded human kidney sections labeling COX2 / Cyclooxygenase 2 with ab23672 at 1/80 dilution. Slides were incubated with biotinylated secondary antibody, followed by alkaline phosphatase streptavidin and chromogen (DAB). Antigen retrieval was heat mediated using citrate buffer pH 6.0.
References (12)
ab23672 has been referenced in 12 publications.
- Huang XL et al. Anti-inflammatory and antinociceptive effects of active ingredients in the essential oils from Gynura procumbens, a traditional medicine and a new and popular food material. J Ethnopharmacol 239:111916 (2019). PubMed: 31034956
- Wang Z et al. Avicularin ameliorates human hepatocellular carcinoma via the regulation of NF-?B/COX-2/PPAR-? activities. Mol Med Rep 19:5417-5423 (2019). PubMed: 31059053
- Chen Y et al. Pretreatment of ghrelin protects H9c2 cells against hypoxia/reoxygenation-induced cell death via PI3K/AKT and AMPK pathways. Artif Cells Nanomed Biotechnol 47:2179-2187 (2019). PubMed: 31159591
- Qiu L et al. miR-155 Modulates Cockroach Allergen- and Oxidative Stress-Induced Cyclooxygenase-2 in Asthma. J Immunol 201:916-929 (2018). PubMed: 29967100
- Juban G et al. AMPK Activation Regulates LTBP4-Dependent TGF-ß1 Secretion by Pro-inflammatory Macrophages and Controls Fibrosis in Duchenne Muscular Dystrophy. Cell Rep 25:2163-2176.e6 (2018). PubMed: 30463013
